From c8742cca8b346ef6a56b9604a25d3ff7e777ab64 Mon Sep 17 00:00:00 2001 From: Schon Date: Mon, 25 Sep 2023 16:56:12 +0200 Subject: [PATCH] default to discarding noncanonical junctions Replaced --remove_noncanonical with --allow_noncanonical --- bookend/core/argument_parsers.py | 2 +- bookend/core/bam_to_elr.py | 11 ++++------- .../bookend/core/argument_parsers.py | 2 +- .../bookend/core/bam_to_elr.py | 11 ++++------- dist/bookend_rna-1.2.1-py3.7-linux-x86_64.egg | Bin 6723118 -> 6723107 bytes 5 files changed, 10 insertions(+), 16 deletions(-) diff --git a/bookend/core/argument_parsers.py b/bookend/core/argument_parsers.py index 62192eb..51b39ec 100644 --- a/bookend/core/argument_parsers.py +++ b/bookend/core/argument_parsers.py @@ -124,7 +124,7 @@ def run(self): bam_to_elr_parser.add_argument("--record_artifacts", dest='RECORD_ARTIFACTS', default=False, action='store_true', help="Reports artifact-masked S/E labels as >/].") bam_to_elr_parser.add_argument("--split", dest='SPLIT', default=False, action='store_true', help="Separate reads into different files by their multimapping number.") bam_to_elr_parser.add_argument("--untrimmed", dest='UNTRIMMED', default=False, action='store_true', help="(overrides -s -c -e) End labels were not trimmed from input reads prior to alignment.") -bam_to_elr_parser.add_argument("--remove_noncanonical", dest='REMOVE_NONCANONICAL', default=False, action='store_true', help="Require canonical splice junction motifs (GT/AG, GC/AG, AT/AC).") +bam_to_elr_parser.add_argument("--allow_noncanonical", dest='ALLOW_NONCANONICAL', default=False, action='store_true', help="Do not require canonical splice junction motifs (GT/AG, GC/AG, AT/AC).") bam_to_elr_parser.add_argument("--sj_shift", dest='SJ_SHIFT', default=0, type=int, help="Shift up to this many bases to find a canonical splice junction") bam_to_elr_parser.add_argument("--minlen_strict", dest='MINLEN_STRICT', default=18, type=int, help="Keep reads down to this length only if perfectly aligned.") bam_to_elr_parser.add_argument("--minlen_loose", dest='MINLEN_LOOSE', default=25, type=int, help="Keep reads down to this length if they passed alignment parameters.") diff --git a/bookend/core/bam_to_elr.py b/bookend/core/bam_to_elr.py index 7c38565..083e161 100644 --- a/bookend/core/bam_to_elr.py +++ b/bookend/core/bam_to_elr.py @@ -43,7 +43,7 @@ def __init__(self, args): self.input = args['INPUT'] self.error_rate = args['ERROR_RATE'] self.max_intron = args['MAX_INTRON'] - self.remove_noncanonical = args['REMOVE_NONCANONICAL'] + self.allow_noncanonical = args['ALLOW_NONCANONICAL'] if self.start or self.end or self.capped: self.stranded = True @@ -92,7 +92,7 @@ def __init__(self, args): 'mismatch_rate':self.mismatch_rate, 'error_rate' : self.error_rate, 'sj_shift':self.sj_shift, - 'remove_noncanonical':self.remove_noncanonical, + 'remove_noncanonical':not self.allow_noncanonical, 'labels_are_trimmed':not self.untrimmed, 'quality_filter':True, 'reference':self.reference, @@ -114,7 +114,6 @@ def __init__(self, args): self.config_dict['max_headclip'] = 10 self.config_dict['quality_filter'] = False self.config_dict['error_rate'] = 0.2 - self.config_dict['remove_noncanonical'] = True self.config_dict['remove_gapped_termini'] = True if self.untrimmed: self.config_dict['max_headclip'] = 120 @@ -129,12 +128,10 @@ def __init__(self, args): self.config_dict['s_tag'] = True self.config_dict['e_tag'] = True self.config_dict['quality_filter'] = False - self.config_dict['remove_noncanonical'] = True self.config_dict['remove_gapped_termini'] = True elif self.data_type.upper() in ['ONT-RNA','ONT_RNA','DIRECT_RNA', 'DIRECT-RNA']: """Reads are from Oxford Nanopore direct RNA kit, downstream of basecalling.""" self.config_dict['stranded'] = True - self.config_dict['remove_noncanonical'] = True self.config_dict['remove_gapped_termini'] = True self.config_dict['labels_are_trimmed'] = False self.config_dict['quality_filter'] = False @@ -250,7 +247,7 @@ def display_options(self): options_string += " *** Filters ***\n" options_string += " --record_artifacts: {}\n".format(self.record_artifacts) options_string += " --mismatch_rate: {}\n".format(self.mismatch_rate) - options_string += " --remove_noncanonical: {}\n".format(self.remove_noncanonical) + options_string += " --allow_noncanonical: {}\n".format(self.allow_noncanonical) options_string += " Perfect minlen (--minlen_strict): {}\n".format(self.minlen_strict) options_string += " Relaxed minlen (--minlen_loose): {}\n".format(self.minlen_loose) options_string += " Secondary alignments (--secondary): {}\n".format(self.secondary) @@ -258,7 +255,7 @@ def display_options(self): if not self.genome: options_string += "\nWARNING: cap detection and artifact masking can only be done if a reference genome is provided." options_string += "\nProvide a genome fasta with --genome /path/to/fasta" - if self.remove_noncanonical: + if self.allow_noncanonical: options_string += "\nWARNING: noncanonical splice junctions can only be detected if --genome is provided." if not self.splice and not self.reference and self.sj_shift: diff --git a/build/lib.linux-x86_64-3.7/bookend/core/argument_parsers.py b/build/lib.linux-x86_64-3.7/bookend/core/argument_parsers.py index 62192eb..51b39ec 100644 --- a/build/lib.linux-x86_64-3.7/bookend/core/argument_parsers.py +++ b/build/lib.linux-x86_64-3.7/bookend/core/argument_parsers.py @@ -124,7 +124,7 @@ def run(self): bam_to_elr_parser.add_argument("--record_artifacts", dest='RECORD_ARTIFACTS', default=False, action='store_true', help="Reports artifact-masked S/E labels as >/].") bam_to_elr_parser.add_argument("--split", dest='SPLIT', default=False, action='store_true', help="Separate reads into different files by their multimapping number.") bam_to_elr_parser.add_argument("--untrimmed", dest='UNTRIMMED', default=False, action='store_true', help="(overrides -s -c -e) End labels were not trimmed from input reads prior to alignment.") -bam_to_elr_parser.add_argument("--remove_noncanonical", dest='REMOVE_NONCANONICAL', default=False, action='store_true', help="Require canonical splice junction motifs (GT/AG, GC/AG, AT/AC).") +bam_to_elr_parser.add_argument("--allow_noncanonical", dest='ALLOW_NONCANONICAL', default=False, action='store_true', help="Do not require canonical splice junction motifs (GT/AG, GC/AG, AT/AC).") bam_to_elr_parser.add_argument("--sj_shift", dest='SJ_SHIFT', default=0, type=int, help="Shift up to this many bases to find a canonical splice junction") bam_to_elr_parser.add_argument("--minlen_strict", dest='MINLEN_STRICT', default=18, type=int, help="Keep reads down to this length only if perfectly aligned.") bam_to_elr_parser.add_argument("--minlen_loose", dest='MINLEN_LOOSE', default=25, type=int, help="Keep reads down to this length if they passed alignment parameters.") diff --git a/build/lib.linux-x86_64-3.7/bookend/core/bam_to_elr.py b/build/lib.linux-x86_64-3.7/bookend/core/bam_to_elr.py index 7c38565..083e161 100644 --- a/build/lib.linux-x86_64-3.7/bookend/core/bam_to_elr.py +++ b/build/lib.linux-x86_64-3.7/bookend/core/bam_to_elr.py @@ -43,7 +43,7 @@ def __init__(self, args): self.input = args['INPUT'] self.error_rate = args['ERROR_RATE'] self.max_intron = args['MAX_INTRON'] - self.remove_noncanonical = args['REMOVE_NONCANONICAL'] + self.allow_noncanonical = args['ALLOW_NONCANONICAL'] if self.start or self.end or self.capped: self.stranded = True @@ -92,7 +92,7 @@ def __init__(self, args): 'mismatch_rate':self.mismatch_rate, 'error_rate' : self.error_rate, 'sj_shift':self.sj_shift, - 'remove_noncanonical':self.remove_noncanonical, + 'remove_noncanonical':not self.allow_noncanonical, 'labels_are_trimmed':not self.untrimmed, 'quality_filter':True, 'reference':self.reference, @@ -114,7 +114,6 @@ def __init__(self, args): self.config_dict['max_headclip'] = 10 self.config_dict['quality_filter'] = False self.config_dict['error_rate'] = 0.2 - self.config_dict['remove_noncanonical'] = True self.config_dict['remove_gapped_termini'] = True if self.untrimmed: self.config_dict['max_headclip'] = 120 @@ -129,12 +128,10 @@ def __init__(self, args): self.config_dict['s_tag'] = True self.config_dict['e_tag'] = True self.config_dict['quality_filter'] = False - self.config_dict['remove_noncanonical'] = True self.config_dict['remove_gapped_termini'] = True elif self.data_type.upper() in ['ONT-RNA','ONT_RNA','DIRECT_RNA', 'DIRECT-RNA']: """Reads are from Oxford Nanopore direct RNA kit, downstream of basecalling.""" self.config_dict['stranded'] = True - self.config_dict['remove_noncanonical'] = True self.config_dict['remove_gapped_termini'] = True self.config_dict['labels_are_trimmed'] = False self.config_dict['quality_filter'] = False @@ -250,7 +247,7 @@ def display_options(self): options_string += " *** Filters ***\n" options_string += " --record_artifacts: {}\n".format(self.record_artifacts) options_string += " --mismatch_rate: {}\n".format(self.mismatch_rate) - options_string += " --remove_noncanonical: {}\n".format(self.remove_noncanonical) + options_string += " --allow_noncanonical: {}\n".format(self.allow_noncanonical) options_string += " Perfect minlen (--minlen_strict): {}\n".format(self.minlen_strict) options_string += " Relaxed minlen (--minlen_loose): {}\n".format(self.minlen_loose) options_string += " Secondary alignments (--secondary): {}\n".format(self.secondary) @@ -258,7 +255,7 @@ def display_options(self): if not self.genome: options_string += "\nWARNING: cap detection and artifact masking can only be done if a reference genome is provided." options_string += "\nProvide a genome fasta with --genome /path/to/fasta" - if self.remove_noncanonical: + if self.allow_noncanonical: options_string += "\nWARNING: noncanonical splice junctions can only be detected if --genome is provided." if not self.splice and not self.reference and self.sj_shift: diff --git 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