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config.yaml
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---
blast:
evalue: 1e-10
maxTargets: "1"
outFmt: "6 qseqid evalue length mismatch salltitles"
deseq2:
maxfraction: "0.7"
fastp:
qualified_quality_phred: 15
filter_for_plots:
average_samples: "yes"
maximum_pvalue: "0.05"
minimum_foldchange: "2"
minimum_reads: "100"
make_plots:
colour_of_heatmap: "c(\"white\",\"green\",\"green4\",\"violet\",\"purple\")"
height_in_dendrogram: "1.5"
membership_min: "0.2"
method_of_clustering: pam
number_of_clusters: "2"
opt_clust_number: average_silhouette_width
refs:
annotation: annotations/Petunia_axillaris_v1.6.2_proteins.mapman_annotation.txt.gz
genome: "ftp://ftp.solgenomics.net/genomes/Petunia_axillaris/assembly/Petunia_axillaris_v1.6.2_genome.fasta"
proteins_fasta: "ftp://ftp.solgenomics.net/genomes/Petunia_axillaris/annotation/Petunia_axillaris_v1.6.2_proteins.fasta"
transcriptome_gtf: "ftp://ftp.solgenomics.net/genomes/Petunia_axillaris/annotation/Petunia_axillaris_v1.6.2_gene_models.gff"
result_dir: results/
trimmomatic:
LeadMinTrimQual: "3"
TrailMinTrimQual: "3"
adapters: adapters.fasta
avgMinQual: "15"
minReadLength: "40"
palindromeClipTreshold: "30"
phred: "-phred33"
seedMisMatches: "2"
simpleClipThreshold: "10"
windowSize: "4"
units: samples.tsv
working_dir: temp/