All notable changes will be documented in this file.
crYOLO
- module wrapping crYOLO - DL tool, based on You Only Look Once (YOLO) object detection system for predicting particle coordinates (basically picking them) in cryo-electron tomograms
IsoNet
: added possibility to post-process IsoNet-corrected tomograms using NAD filteringDynamoTemplateMatching
: enabled usage of the tomograms from the custom user-provided pathAreTomo
:- enabled direct usage of AreTomo alignment parameters from previously executed AreTomo step
- enabled parameters: tilt axis offset (pre-tilt) and temporary reconstruction thickness (VolZ)
DynamoTemplateMatching
: fixed bug preventing GPU-enabled speedup
- changed license of the core code base from
AGPLv3
toGPLv3
- changed license to a bit more permissive version in order to allow software maintainers and other TomoBEAR contributors more flexibility in terms of TomoBEAR modifications, needed to provide a TomoBEAR operation over local networks (like institutional intranets and/or shared filesystems); - updated documentation:
- recorded a set of short 8-12 min video-tutorials on TomoBEAR installation and 80S ribosome (EMPIAR-10064) tutorial;
- added contribution guidelines and tips
- extended "Usage" and reviewed "Installation and Setup" sections;
- added "External Software" section for dependencies installation/citation description.
local_live
data processing mode - new mode allows to make on-the-fly pre-processing and reconstructions during data collection to check the sample quality. Currently only single-shot collected data is supported for this feature.EER
(Electron Event Representation) support: input files format perception along with.gain
gain file format support are now enabled inMotionCor2
moduleGenerateParticles
- new module which allows to generate particles using either Dynamo (by cropping) or SUSAN (by subtomogram reconstructions)IsoNet
- module wrapping IsoNet - CNN for denoising and missing wedge reconstruction
BinStacks
: added possibility to bin non-aligned stacksAreTomo
:- enabled binned stack input
- enabled local patch-based alignment
BatchRunTomo
: enabled IMOD-based patch-trackingReconstruct
: enabled nonlinear anisotropic diffusion (NAD) filter for tomograms post-filteringGCTFCtfphaseflipCTFCorrection
: added possibility to use CTFFIND4
- input files perception - fixed duplicated file extension ( issue #16 )
GridEdgeEraser
: a new module which allows to identify position of the grid edge and mask it out.- Currently only gold grids data is supported for this feature.
AreTomo
:- enabled IMOD-compatible files output
- enabled tilt axis offset parameter
EMDTemplateGeneration
: fixed EMDB URLBatchRunTomo
: fixed bug which prevented running TomoBEAR project for a single tilt serieGCTFCtfphaseflipCTFCorrection
:- fixed AreTomo-aligned data usage
- fixed aligned stack usage
- fixed Ctfphaseflip usage
Reconstruct
: fixed reconstruction based on AreTomo-aligned data
AreTomo
module: fixed parallelization and usage