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哥们 是刚刚接触生信分析的,还请大佬多多指教 |
哈哈哈哈谢谢,好久没更新了没想到还有人看......
DESeq2是做普通的二代转录组差异表达基因的,seruat做单细胞转录组的,cellranger也是单细胞用的,单细胞这方面我么有经验,现在主要做各种基因组分析~
谢文龙
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发件人: "Phantom-Aria/Phantom-Aria.github.io" ***@***.***>;
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主题: [Phantom-Aria/Phantom-Aria.github.io] 评论 (Issue #1)
意外发现了一个很牛的生信大佬!
首先发自内心的赞叹哥们你的博客做的是真不错,其次我有个问题
DESeq2不应该是蛋白组学用的R包吗,转录组学用seruat是不是更好>.<
然后在比对方面,我用hisat尝试生成的sam文件高达100g(甚至还没有跑完),目前用的是cellranger在进行参考基因组。会不会好一点(主要他还能生成一些例如Reads Mapped Confidently to Genome真的太香了)
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好嘞谢谢dalao ♪(・ω・)ノ |
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意外发现了一个很牛的生信大佬!
首先发自内心的赞叹哥们你的博客做的是真不错,其次我有个问题
DESeq2不应该是蛋白组学用的R包吗,转录组学用seruat是不是更好>.<
然后在比对方面,我用hisat尝试生成的sam文件高达100g(甚至还没有跑完),目前用的是cellranger在进行参考基因组。会不会好一点(主要他还能生成一些例如Reads Mapped Confidently to Genome真的太香了)
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