Take too long time to runing Taxonomy using UNITE database

[Danyang1111]

Hi,

I am doing the assigntaxonomy step.
I use the UNITE database Full "UNITE+INSD" dataset:https://unite.ut.ee/repository.php

the code:
unite.ref <- "/home/dh23063/UNITE_public_21.04.2024.fasta.gz"
taxa <- assignTaxonomy(seqtab.nochim, unite.ref, multithread = TRUE, tryRC = TRUE)

And for now I wait for 2 hours but it still not finish running.
is it normal? does the database running in a right way, why it takes so long time?

Thanks.

[sadia-marjia]

I am facing the same issue.

[benjjneb]

The appropriate files to use with assignTaxonomy are the General Fasta releases (with 10s of thousands - 200k sequences), not the UNITE+INSD relsease (with 2-8M sequences).