Following ITS dada2 cutadapt trimming, why is a minimum length of 50 used?

[kdbchau]

Is there a reference to justify why the minLen was set to 50 after adaptor removal? I have searched a lot of journals but I don't see any recommended minimum to use for ITS (or usually the minimum is much higher >100bp).

[benjjneb]

It's to remove very low-length sequences that sometimes come out of cutadapt.

If you know the length distribution of your target locus across all relevant taxa, you can adjust this higher if you want.

[kdbchau]

Is there a citation we can use for your ITS DADA2 1.8 workflow?

[benjjneb]

Just the webpage itself.