diff --git a/recipes/relion/meta.yaml b/recipes/relion/meta.yaml
index 529511c1c39a1..d5cf58a1b6629 100644
--- a/recipes/relion/meta.yaml
+++ b/recipes/relion/meta.yaml
@@ -1,4 +1,4 @@
-{% set version = "4.0.2" %}
+{% set version = "5.0.0" %}
 
 package:
   name: relion
@@ -6,10 +6,10 @@ package:
 
 source:
   url: https://github.com/3dem/relion/archive/refs/tags/{{ version }}.tar.gz
-  sha256: 7ccc941a6a885bd850efa8867ea908254d8dc260cf72cc24c375bb9f1d56bf91
+  sha256: 5d02d529bfdb396204310b35963f35e5ec40ed9fd10bc88c901119ae7d7739fc
 
 build:
-  number: 2
+  number: 0
   run_exports:
     - {{ pin_subpackage('relion', max_pin='x') }}