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-M parameter for BWA MEM alignment #144

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aariani opened this issue Jan 4, 2021 · 0 comments
Open

-M parameter for BWA MEM alignment #144

aariani opened this issue Jan 4, 2021 · 0 comments

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@aariani
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aariani commented Jan 4, 2021

Thanks a lot for developing this pipeline. I have a question about BWA MEM alignment. In your main paper you mention that:

SpeedSeq aligns paired-end FASTQ files to the human GRCh37 reference genome with BWA-MEM, using the “-M” flag to mark shorter alignments as secondary

However by looking into the code it seems that you just use BWA MEM with the -t -R and -I options

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