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多样本问题如何解决? #3

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25343 opened this issue Jul 10, 2024 · 2 comments
Open

多样本问题如何解决? #3

25343 opened this issue Jul 10, 2024 · 2 comments

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@25343
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25343 commented Jul 10, 2024

Junjun大神您好!
在我看到关于“普通的Bulk转录组也能做拟时序分析” 的推文时,正感到新奇,没想到您就已经做出R包了,对您的开发速度赶到无比惊讶。同时我也是您R包ClusterGVis的忠实粉丝。

但在我使用bulkPseudotime,我遇到了一个问题,我的数据在同一时间点具有多个样本。但是拟时序的时候,拟合产生的新的 PCA 分布只会显示部分样本。
微信图片_20240710160012
这个问题该如何解决?

@25343
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25343 commented Jul 10, 2024

您可以看到这里是两个时间节点,多个样本。但是似乎理解成了每个样本是一个时间节点,并且显示不完全。

@junjunlab
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应该是颜色不够,用ggplot语法加点颜色,psetime_res@sample_pca_plot[[x]] + scale_color_manuals(values = 你的颜色)

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