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Dear nanoporetech team,
I have this weird error that happens everytime i try to run your pipeline. Even using config file that already worked before it fails and gives this error:
AttributeError in line 5 of /data/hilgers/group/alfonso/projects/2021_LRS_paper/bin/pipeline-polya-ng/Snakefile:
'Workflow' object has no attribute 'overwrite_configfiles'
Its very important for my project I would appreciate the help this is my config file:
General pipeline parameters:
Name of the pipeline:
pipeline: "pipeline-polya-ng3"
ABSOLUTE path to directory holding the working directory:
Dear nanoporetech team,
I have this weird error that happens everytime i try to run your pipeline. Even using config file that already worked before it fails and gives this error:
AttributeError in line 5 of /data/hilgers/group/alfonso/projects/2021_LRS_paper/bin/pipeline-polya-ng/Snakefile:
'Workflow' object has no attribute 'overwrite_configfiles'
Its very important for my project I would appreciate the help this is my config file:
General pipeline parameters:
Name of the pipeline:
pipeline: "pipeline-polya-ng3"
ABSOLUTE path to directory holding the working directory:
workdir_top: "/data/hilgers/group/alfonso/projects/2021_LRS_paper/data/oxford_nanopore/rna_direct/polyATailLengths"
Repository URL:
repo: "https://github.com/nanoporetech/pipeline-polya-ng"
Pipeline-specific parameters:
Transcriptome fasta file
transcriptome: "/data/x/group/alfonso/projects/2021_LRS_paper/data/cia_isoforms/meta_cia_transcriptome/sqanti/filt.noise.keep.fusion.ensGeneNames.cia_tissues.sqanti_corrected.fasta"
Directory with FAST5 files
fast5_dir: "/data/x/sequencing_data/OxfordNanopore/20210208_1250_X3_FAP20660_3a50e0e9/fast5_pass"
Directory with fastq files
fastq_dir: "/data/x/group/alfonso/projects/2021_LRS_paper/data/oxford_nanopore/rna_direct/polyATailLengths/fastq/w1118_ovaries_R3_RNAD.fastq.gz"
Directory with sequencing summary files
summary_dir: "/data/x/sequencing_data/OxfordNanopore/20210208_1250_X3_FAP20660_3a50e0e9/Project_1709_Alfonso_Hilgers/sequencing_summary.txt"
Fasta file containing spike-ins
spikein_fasta: "/data/x/group/alfonso/projects/2021_LRS_paper/repo/ERCC92.fa"
Minimum mapping quality
min_mapping_qual: 5
Plot per-transcript plots
per_transcript_plots: "true"
Number of threads to use
threads: 50
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