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AttributeError i #1

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cag134392 opened this issue Jun 1, 2021 · 0 comments
Open

AttributeError i #1

cag134392 opened this issue Jun 1, 2021 · 0 comments

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@cag134392
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Dear nanoporetech team,
I have this weird error that happens everytime i try to run your pipeline. Even using config file that already worked before it fails and gives this error:

AttributeError in line 5 of /data/hilgers/group/alfonso/projects/2021_LRS_paper/bin/pipeline-polya-ng/Snakefile:
'Workflow' object has no attribute 'overwrite_configfiles'

Its very important for my project I would appreciate the help this is my config file:


General pipeline parameters:

Name of the pipeline:

pipeline: "pipeline-polya-ng3"

ABSOLUTE path to directory holding the working directory:

workdir_top: "/data/hilgers/group/alfonso/projects/2021_LRS_paper/data/oxford_nanopore/rna_direct/polyATailLengths"

Repository URL:

repo: "https://github.com/nanoporetech/pipeline-polya-ng"

Pipeline-specific parameters:

Transcriptome fasta file

transcriptome: "/data/x/group/alfonso/projects/2021_LRS_paper/data/cia_isoforms/meta_cia_transcriptome/sqanti/filt.noise.keep.fusion.ensGeneNames.cia_tissues.sqanti_corrected.fasta"

Directory with FAST5 files

fast5_dir: "/data/x/sequencing_data/OxfordNanopore/20210208_1250_X3_FAP20660_3a50e0e9/fast5_pass"

Directory with fastq files

fastq_dir: "/data/x/group/alfonso/projects/2021_LRS_paper/data/oxford_nanopore/rna_direct/polyATailLengths/fastq/w1118_ovaries_R3_RNAD.fastq.gz"

Directory with sequencing summary files

summary_dir: "/data/x/sequencing_data/OxfordNanopore/20210208_1250_X3_FAP20660_3a50e0e9/Project_1709_Alfonso_Hilgers/sequencing_summary.txt"

Fasta file containing spike-ins

spikein_fasta: "/data/x/group/alfonso/projects/2021_LRS_paper/repo/ERCC92.fa"

Minimum mapping quality

min_mapping_qual: 5

Plot per-transcript plots

per_transcript_plots: "true"

Number of threads to use

threads: 50

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