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I am trying to run nf-core/riboseq on a cluster computer, but it fails on the sortMeRNA step for all samples.
Command used and terminal output
I run this nextflow command:
$ nextflow run nf-core/riboseq -profile computerome --project reg_00024 --input nf-core_riboseq_sample_sheet.csv --outdir /home/projects/reg_00024/data/RiboSeq/nf-core_riboseq_out/ --fasta /home/projects/reg_00024/data/RiboSeq/ref/Homo_sapiens.GRCh38.dna_sm.primary_assembly.fa.gz --gtf /home/projects/reg_00024/data/RiboSeq/ref/Homo_sapiens.GRCh38.113.gtf.gz --save_reference -resumeThis is one of the error messages from the .nextflow.log file:Jan-09 16:39:14.631 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[jobId: 51991560; id: 61; name: NFCORE_RIBOSEQ:RIBOSEQ:PREPROCESS_RNASEQ:SORTMERNA (0279_05_RiboSeq); status: COMPLETED; exit: 137; error: -; workDir: /home/projects/reg_00024/data/RiboSeq/work/e1/24e9e6f1ba0dd9c49c905846f6bf53 started: 1736431257223; exited: 2025-01-09T15:39:04.322921661Z; ]Jan-09 16:39:14.636 [TaskFinalizer-6] DEBUG nextflow.processor.TaskProcessor - Handling unexpected condition for task: name=NFCORE_RIBOSEQ:RIBOSEQ:PREPROCESS_RNASEQ:SORTMERNA (0279_05_RiboSeq); work-dir=/home/projects/reg_00024/data/RiboSeq/work/e1/24e9e6f1ba0dd9c49c905846f6bf53 error [nextflow.exception.ProcessFailedException]: Process `NFCORE_RIBOSEQ:RIBOSEQ:PREPROCESS_RNASEQ:SORTMERNA (0279_05_RiboSeq)` terminated with an error exit status (137)Jan-09 16:39:14.638 [TaskFinalizer-6] INFO nextflow.processor.TaskProcessor - [e1/24e9e6] NOTE: Process `NFCORE_RIBOSEQ:RIBOSEQ:PREPROCESS_RNASEQ:SORTMERNA (0279_05_RiboSeq)` terminated with an error exitstatus (137) -- Execution is retried (2)I have tried allocating 256Gb memory in the .command.run file and running this (after removing the kvdb-folder):
$ bash .command.runBut I get this similar error message:[is_split_ready:726] found existing readfeed descriptor ./readb/readfeed/home/projects/reg_00024/data/BAT_RiboSeq_Taylah/work/e1/24e9e6f1ba0dd9c49c905846f6bf53/.command.sh: line 11: 41 Aborted (core dumped) sortmerna --ref rfam-5.8s-database-id98.fasta --ref rfam-5s-database-id98.fasta --ref silva-arc-16s-id95.fasta --ref silva-arc-23s-id98.fasta --ref silva-bac-16s-id90.fasta --ref silva-bac-23s-id98.fasta --ref silva-euk-18s-id95.fasta --ref silva-euk-28s-id98.fasta --reads 0279_05_RiboSeq_trimmed.fq.gz --threads 24 --workdir . --aligned rRNA_reads --fastx --other non_rRNA_reads --num_alignments 1 -v --index 0INFO: Cleaning up image...I have also tried running the sortMeRNA command directly from the .command.sh file (after loading the sortmerna/4.3.6 module):
$ sortmerna \ --ref rfam-5.8s-database-id98.fasta --ref rfam-5s-database-id98.fasta --ref silva-arc-16s-id95.fasta --ref silva-arc-23s-id98.fasta --ref silva-bac-16s-id90.fasta --ref silva-bac-23s-id98.fasta --ref silva-euk-18s-id95.fasta --ref silva-euk-28s-id98.fasta \ \ --reads 0279_05_RiboSeq_trimmed.fq.gz \ --threads 24 \ --workdir . \ --aligned rRNA_reads --fastx --other non_rRNA_reads \ \ \ --num_alignments 1 -v --index 0This gives me this error message:[is_split_ready:726] found existing readfeed descriptor ./readb/readfeedterminate called after throwing an instance of 'std::out_of_range' what(): stoiAborted (core dumped)This seems to be similar to the sortMeRNA bug described here:https://github.com/sortmerna/sortmerna/issues/379Can you please advice on how to preceed and if the sortMeRNA issue in the link is relevant/how to implement the solution?I am fairly new to NextFlow, so please let me know of what info might be missing.
Relevant files
No response
System information
Nextflow version: 24.10.3
Hardware: HPC
Executor: TORQUE
Container engine: Singularity
OS: CentOS Linux release 7.6.1810
Version of nf-core/riboseq: 1.0.1
The text was updated successfully, but these errors were encountered:
Description of the bug
I am trying to run nf-core/riboseq on a cluster computer, but it fails on the sortMeRNA step for all samples.
Command used and terminal output
Relevant files
No response
System information
Nextflow version: 24.10.3
Hardware: HPC
Executor: TORQUE
Container engine: Singularity
OS: CentOS Linux release 7.6.1810
Version of nf-core/riboseq: 1.0.1
The text was updated successfully, but these errors were encountered: