diff --git a/EvoScriPy/GUI.py b/EvoScriPy/GUI.py index 5febc33..2ad793f 100644 --- a/EvoScriPy/GUI.py +++ b/EvoScriPy/GUI.py @@ -147,6 +147,8 @@ def __init__(self, protocol): def set_firstTip(self, *args): self.protocol.firstTip = self.firstTip_v.get() + if self.protocol.firstTip == "None": + self.protocol.firstTip = None def set_WT_FN(self, *args): self.protocol.worktable_template_filename = self.worktable_filename_v.get() diff --git a/EvoScriPy/labware.py b/EvoScriPy/labware.py index 9b82377..fcbf5bd 100644 --- a/EvoScriPy/labware.py +++ b/EvoScriPy/labware.py @@ -533,6 +533,8 @@ def set_first_pos(self, labw_type_name=None, posstr=None): labw = self.get_DITI_series().current assert labw, "Failed to find first DITI position." + if posstr is None: + posstr = "" pos = posstr.split('-') @@ -983,25 +985,26 @@ def autoselect(self, offset=0, maxTips=1, replys=1): # OK make this "virtual". self.selectOnly(range(offset, offset + maxTips)) return maxTips - def offset(self, row, col=1): - if isinstance(row, str): - assert col == 1, "Please, define the column only in the row string." - return self.offsetFromName(row) + def offset(self, row_pos, col=1): + assert row_pos is not None + if isinstance(row_pos, Well): + assert row_pos.labware is self, "This is a well from another labware." + return row_pos.offset - if isinstance(row, Well): - assert row.labware is self, "This is a well from another labware." - return row.offset + if isinstance(row_pos, str): + assert col == 1, "Please, define the column only in the row string." + return self.offsetFromName(row_pos) - if isinstance(row, Labware.Position): - col = row.col - row = row.row + if isinstance(row_pos, Labware.Position): + col = row_pos.col + row_pos = row_pos.row - if isinstance(row, int): - return row - 1 + (col - 1) * self.type.nRow + if isinstance(row_pos, int): + return row_pos - 1 + (col - 1) * self.type.nRow - assert len(row.replicas) == 1, "Failed to assume a Reagent with only one replica (aliquot)" - return self.offset(row) + assert len(row_pos.replicas) == 1, "Failed to assume a Reagent with only one replica (aliquot)" + return self.offset(row_pos, col) # assert False, "Unknow row type" diff --git a/protocols/Prefill_plates_VEW1_ElutionBuffer_VEW2/scripts/Prefill_plates_VEW1_ElutionBuffer_VEW2.esc b/protocols/Prefill_plates_VEW1_ElutionBuffer_VEW2/scripts/Prefill_plates_VEW1_ElutionBuffer_VEW2.esc new file mode 100644 index 0000000..b6f7926 --- /dev/null +++ b/protocols/Prefill_plates_VEW1_ElutionBuffer_VEW2/scripts/Prefill_plates_VEW1_ElutionBuffer_VEW2.esc @@ -0,0 +1,264 @@ +19399F68 +20190306_122833 Admin + +Administrator +--{ RES }-- +V;200 +--{ CFG }-- +999;219;32; +14;-1;5;-1;-1;-1;-1;-1;16;312;313;-1;-1;-1;-1;-1;37;-1;-1;-1;-1;-1;-1;-1;104;-1;12;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1; +998;0; +998;3;DiTi 1000ul;DiTi 1000ul;DiTi 1000ul; +998;1000-1;1000-2;1000-3; +998;0; +998;0; +998;0; +998;0; +998;0; +998;3;;Trough 100ml;Trough 100ml; +998;;2-Vl Lysis Buffer;3-VEB Binding Buffer; +998;16;Tube Greinerconic 2mL 16 Pos;;;;;;;;;;;;;;;; +998;Reactives;;;;;;;;;;;;;;;; +998;1;Tube Eppendorf 6x 16 Pos; +998;Proben; +998;0; +998;0; +998;0; +998;0; +998;0; +998;2;Tube Eppendorf 48 Pos;Tube Eppendorf 48 Pos; +998;48 Pos Heat;48PosMagnet; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;8;Washstation 2Grid Cleaner short;Washstation 2Grid Waste;Washstation 2Grid Cleaner long;Trough 100ml;Trough 100ml;Trough 100ml;Washstation 2Grid DiTi Waste;; +998;;;;4-VEW1 Wash Buffe;1-VEL-ElutionBuffer;5-VEW2-WashBuffer;;; +998;0; +998;3;96 Well DeepWell square;96 Well Microplate;96 Well DeepWell square; +998;Plate VEW1;Plate ElutB;Plate VEW2; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;0; +998;2; +998;4;0;System; +998;4;1;Te-MagS portrait; +998;1; +998;37;Tube Eppendorf 48 Pos; +998;1; +998;15; +996;0;0; +--{ RPG }-- +Comment("Prefill plates with VEW1, Elution buffer and VEW2 for 96 samples."); +Wash(15,23,1,23,0,"30",50,"10",50,0.00,50,100,1,0,0,0); +Group("Prefill plates with VEW1, Elution buffer and VEW2"); +UserPrompt("Put the plates for VEW1, Elution buffer and VEW2 in that order",1,-1); +Group("Distribute: 100.0 L of Elution Buffer "); +Comment("Distribute: 100.0 L of Elution Buffer (9984.0 L total) from [grid:23 site:5 ['well 1 in labware 1-VEL-ElutionBuffer: with 9984.0 uL of reagent Elution Buffer '] into Plate ElutB[grid:25 site:2] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]:"); +GetDITI2(15,"DiTi 1000ul",0,0,0,300); +Aspirate(15,"Water free cuvette","900.00","900.00","900.00","900.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08?0000000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080N000000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C0800l00000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000100000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000030000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08000000p7000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C0800000000?00000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08000000000N0000",0,0); +Aspirate(15,"Water free cuvette","900.00","900.00","900.00","900.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000l000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000001",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000000N",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08000000000000?0",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000p700",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000003000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000010000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08000000l0000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C0800000N00000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000?000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C0800p70000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080300000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C081000000000000",0,0); +GroupEnd(); +Group("Distribute: 600.0 L of VEW2 - WashBuffer "); +Comment("Distribute: 600.0 L of VEW2 - WashBuffer (59904.0 L total) from [grid:23 site:6 ['well 1 in labware 5-VEW2-WashBuffer: with 59904.0 uL of reagent VEW2 - WashBuffer '] into Plate VEW2[grid:25 site:3] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]:"); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08?0000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080N000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C0800l00000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000100000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000030000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08000000p7000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C0800000000?00000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08000000000N0000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000l000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000001",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000000N",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08000000000000?0",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000p700",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000003000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000010000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08000000l0000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C0800000N00000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000?000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C0800p70000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080300000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C081000000000000",0,0); +GroupEnd(); +Group("Distribute: 600.0 L of VEW1 - Wash Buffer "); +Comment("Distribute: 600.0 L of VEW1 - Wash Buffer (59904.0 L total) from [grid:23 site:4 ['well 1 in labware 4-VEW1 Wash Buffe: with 59904.0 uL of reagent VEW1 - Wash Buffer '] into Plate VEW1[grid:25 site:1] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]:"); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08?0000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080N000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800l00000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000100000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000030000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000p7000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000000?00000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000N0000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000l000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000001",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000N",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000000?0",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000p700",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000003000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000010000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000l0000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000N00000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000?000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800p70000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080300000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C081000000000000",0,0); +GroupEnd(); +GroupEnd(); +DropDITI(15,23,6,0.00,300,0); diff --git a/protocols/Prefill_plates_VEW1_ElutionBuffer_VEW2/scripts/Prefill_plates_VEW1_ElutionBuffer_VEW2.gwl b/protocols/Prefill_plates_VEW1_ElutionBuffer_VEW2/scripts/Prefill_plates_VEW1_ElutionBuffer_VEW2.gwl new file mode 100644 index 0000000..909fc42 --- /dev/null +++ b/protocols/Prefill_plates_VEW1_ElutionBuffer_VEW2/scripts/Prefill_plates_VEW1_ElutionBuffer_VEW2.gwl @@ -0,0 +1,131 @@ +B;Comment("Prefill plates with VEW1, Elution buffer and VEW2 for 96 samples."); +B;Wash(15,23,1,23,0,"30",50,"10",50,0.00,50,100,1,0,0,0); +B;UserPrompt("Put the plates for VEW1, Elution buffer and VEW2 in that order",1,-1); +B;Comment("Distribute: 100.0 L of Elution Buffer (9984.0 L total) from [grid:23 site:5 ['well 1 in labware 1-VEL-ElutionBuffer: with 9984.0 uL of reagent Elution Buffer '] into Plate ElutB[grid:25 site:2] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]:"); +B;GetDITI2(15,"DiTi 1000ul",0,0,0,300); +B;Aspirate(15,"Water free cuvette","900.00","900.00","900.00","900.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08?0000000000000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080N000000000000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C0800l00000000000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000100000000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000030000000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08000000p7000000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C0800000000?00000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08000000000N0000",0,0); +B;Aspirate(15,"Water free cuvette","900.00","900.00","900.00","900.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000l000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000001",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000000N",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08000000000000?0",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000p700",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000003000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000010000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08000000l0000000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C0800000N00000000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000?000000000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C0800p70000000000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080300000000000",0,0); +B;Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C081000000000000",0,0); +B;Comment("Distribute: 600.0 L of VEW2 - WashBuffer (59904.0 L total) from [grid:23 site:6 ['well 1 in labware 5-VEW2-WashBuffer: with 59904.0 uL of reagent VEW2 - WashBuffer '] into Plate VEW2[grid:25 site:3] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]:"); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08?0000000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080N000000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C0800l00000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000100000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000030000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08000000p7000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C0800000000?00000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08000000000N0000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000l000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000001",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000000N",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08000000000000?0",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000p700",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000003000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000010000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08000000l0000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C0800000N00000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000?000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C0800p70000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080300000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C081000000000000",0,0); +B;Comment("Distribute: 600.0 L of VEW1 - Wash Buffer (59904.0 L total) from [grid:23 site:4 ['well 1 in labware 4-VEW1 Wash Buffe: with 59904.0 uL of reagent VEW1 - Wash Buffer '] into Plate VEW1[grid:25 site:1] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]:"); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08?0000000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080N000000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800l00000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000100000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000030000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000p7000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000000?00000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000N0000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000l000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000001",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000N",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000000?0",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000p700",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000003000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000010000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000l0000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000N00000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000?000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800p70000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080300000000000",0,0); +B;Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +B;Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C081000000000000",0,0); +B;DropDITI(15,23,6,0.00,300,0); diff --git a/protocols/Prefill_plates_VEW1_ElutionBuffer_VEW2/scripts/Prefill_plates_VEW1_ElutionBuffer_VEW2.protocol.txt b/protocols/Prefill_plates_VEW1_ElutionBuffer_VEW2/scripts/Prefill_plates_VEW1_ElutionBuffer_VEW2.protocol.txt new file mode 100644 index 0000000..b39a648 --- /dev/null +++ b/protocols/Prefill_plates_VEW1_ElutionBuffer_VEW2/scripts/Prefill_plates_VEW1_ElutionBuffer_VEW2.protocol.txt @@ -0,0 +1,12 @@ +Prefill plates with VEW1, Elution buffer and VEW2 for 96 samples. + +Prefill plates with VEW1, Elution buffer and VEW2 + + Distribute: 100.0 L of Elution Buffer + Distribute: 100.0 L of Elution Buffer (9984.0 L total) from [grid:23 site:5 ['well 1 in labware 1-VEL-ElutionBuffer: with 9984.0 uL of reagent Elution Buffer '] into Plate ElutB[grid:25 site:2] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]: + + Distribute: 600.0 L of VEW2 - WashBuffer + Distribute: 600.0 L of VEW2 - WashBuffer (59904.0 L total) from [grid:23 site:6 ['well 1 in labware 5-VEW2-WashBuffer: with 59904.0 uL of reagent VEW2 - WashBuffer '] into Plate VEW2[grid:25 site:3] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]: + + Distribute: 600.0 L of VEW1 - Wash Buffer + Distribute: 600.0 L of VEW1 - Wash Buffer (59904.0 L total) from [grid:23 site:4 ['well 1 in labware 4-VEW1 Wash Buffe: with 59904.0 uL of reagent VEW1 - Wash Buffer '] into Plate VEW1[grid:25 site:1] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]: diff --git a/protocols/Prefill_plates_VEW1_ElutionBuffer_VEW2/scripts/Prefill_plates_VEW1_ElutionBuffer_VEW2.txt b/protocols/Prefill_plates_VEW1_ElutionBuffer_VEW2/scripts/Prefill_plates_VEW1_ElutionBuffer_VEW2.txt new file mode 100644 index 0000000..e41d319 --- /dev/null +++ b/protocols/Prefill_plates_VEW1_ElutionBuffer_VEW2/scripts/Prefill_plates_VEW1_ElutionBuffer_VEW2.txt @@ -0,0 +1,139 @@ +Comment("Prefill plates with VEW1, Elution buffer and VEW2 for 96 samples."); +Wash(15,23,1,23,0,"30",50,"10",50,0.00,50,100,1,0,0,0); +Group("Prefill plates with VEW1, Elution buffer and VEW2"); +UserPrompt("Put the plates for VEW1, Elution buffer and VEW2 in that order",1,-1); +Group("Distribute: 100.0 L of Elution Buffer "); +Comment("Distribute: 100.0 L of Elution Buffer (9984.0 L total) from [grid:23 site:5 ['well 1 in labware 1-VEL-ElutionBuffer: with 9984.0 uL of reagent Elution Buffer '] into Plate ElutB[grid:25 site:2] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]:"); +GetDITI2(15,"DiTi 1000ul",0,0,0,300); +Aspirate(15,"Water free cuvette","900.00","900.00","900.00","900.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08?0000000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080N000000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C0800l00000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000100000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000030000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08000000p7000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C0800000000?00000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08000000000N0000",0,0); +Aspirate(15,"Water free cuvette","900.00","900.00","900.00","900.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000l000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000001",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000000N",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08000000000000?0",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000p700",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000003000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000010000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C08000000l0000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C0800000N00000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080000?000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C0800p70000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C080300000000000",0,0); +Dispense(15,"Water free cuvette","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,1,1,"0C081000000000000",0,0); +GroupEnd(); +Group("Distribute: 600.0 L of VEW2 - WashBuffer "); +Comment("Distribute: 600.0 L of VEW2 - WashBuffer (59904.0 L total) from [grid:23 site:6 ['well 1 in labware 5-VEW2-WashBuffer: with 59904.0 uL of reagent VEW2 - WashBuffer '] into Plate VEW2[grid:25 site:3] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]:"); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08?0000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080N000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C0800l00000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000100000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000030000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08000000p7000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C0800000000?00000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08000000000N0000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000l000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000001",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000000N",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08000000000000?0",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000p700",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000003000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000010000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C08000000l0000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C0800000N00000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080000?000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C0800p70000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C080300000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,5,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,2,1,"0C081000000000000",0,0); +GroupEnd(); +Group("Distribute: 600.0 L of VEW1 - Wash Buffer "); +Comment("Distribute: 600.0 L of VEW1 - Wash Buffer (59904.0 L total) from [grid:23 site:4 ['well 1 in labware 4-VEW1 Wash Buffe: with 59904.0 uL of reagent VEW1 - Wash Buffer '] into Plate VEW1[grid:25 site:1] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]:"); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08?0000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080N000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800l00000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000100000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000030000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000p7000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000000?00000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000N0000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000l000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000001",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000N",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000000?0",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000p700",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000003000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000010000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000l0000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000N00000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000?000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800p70000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C080300000000000",0,0); +Aspirate(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,3,1,"0108l0",0,0); +Dispense(15,"Water free cuvette","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,25,0,1,"0C081000000000000",0,0); +GroupEnd(); +GroupEnd(); +DropDITI(15,23,6,0.00,300,0);