From b2f45057faa99500761be0af246c8b82a8ed6a97 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Thu, 28 Feb 2019 16:39:31 +0000
Subject: [PATCH 01/25] Added javadoc

---
 .../components/genebuilder/cv/GoBox.java      | 24 ++++++++++---------
 1 file changed, 13 insertions(+), 11 deletions(-)

diff --git a/src/main/java/uk/ac/sanger/artemis/components/genebuilder/cv/GoBox.java b/src/main/java/uk/ac/sanger/artemis/components/genebuilder/cv/GoBox.java
index fbb293723..1bfa50a2c 100644
--- a/src/main/java/uk/ac/sanger/artemis/components/genebuilder/cv/GoBox.java
+++ b/src/main/java/uk/ac/sanger/artemis/components/genebuilder/cv/GoBox.java
@@ -474,21 +474,24 @@ protected boolean isQualifierChanged()
   
   /**
    * Update the qualifier from the GO form.
-   * @param  qv QualifierVector
+   * @param  qv QualifierVector - Vector of controlled vocab qualifiers
    */
   protected void updateQualifier(final QualifierVector qv)
   {
 	int index = qv.indexOfQualifierWithName(origQualifier.getName());
+	
+	// Get the qualifier containing all the cached GO terms strings
     Qualifier oldQualifier = qv.getQualifierByName(origQualifier.getName());
-    final String origQualifierString = getOrigQualifierString();
     
+    // Get the GO id
+    final String origQualifierString = getOrigQualifierString();
     final String goId = getField("GOid=", origQualifierString);
     
-    // Get the old GO rows
+    // Get the cached GO term strings from the GO qualifier
     StringVector oldValues = oldQualifier.getValues();
     Vector<Integer> values_index = new Vector<Integer>();
     
-    // Find only the "old" GO term rows that match our goId
+    // Find only the cached GO term rows that match our goId
     // and add their indexes to the values_index list.
     for(int i=0; i<oldValues.size(); i++)
     {
@@ -503,14 +506,15 @@ protected void updateQualifier(final QualifierVector qv)
   
     if(values_index.size() > 0)
     { 
-      // We are here, because there were some "old" GO rows
+      // We are here, because there were some cached GO rows
       // that matched the GO id of this term.
     	
-      // Get the GO id at this term's current index from the "old" terms list
+      // Get the GO id at this term's current index from the cached terms list
       String oldValue = oldValues.get(value_index);
       String oldGoId  = getField("GOid=", oldValue);
       
       // Really not sure what the point of this code block is....
+      // Potentially remove in the future.
       if(!goId.equals(oldGoId))
       {
         if(values_index.size() == 1)
@@ -520,7 +524,6 @@ protected void updateQualifier(final QualifierVector qv)
           final String with = getField("with=", origQualifierString);
           final String evidence = getField("evidence=", origQualifierString);
           final String dbxref = getField("dbxref=", origQualifierString);
-          final String source = getField(ASSIGNEDBY_QUALIFIER, origQualifierString);
           for(int i=0; i<values_index.size(); i++)
           {
             int ind = values_index.get(i).intValue();
@@ -544,10 +547,6 @@ protected void updateQualifier(final QualifierVector qv)
             String thisEvidence = getField("evidence=", value);
             if(thisEvidence.equals(evidence))
               break;
-            
-            String thisSource = getField(ASSIGNEDBY_QUALIFIER, value);
-            if(thisSource.equals(source))
-              break;
           }
         }
       }
@@ -555,9 +554,12 @@ protected void updateQualifier(final QualifierVector qv)
     else
       value_index = -99;
 
+    // Remove this GO term from the cached list of GO table rows
     if(value_index > -1)
       oldValues.remove(value_index);
     
+    // Get the latest field values and add in
+    
     String updatedQualifierString = updateQualifierString();
     logger4j.debug(origQualifierString);
     logger4j.debug(updatedQualifierString);

From 0da720b3738b1cac0acfd0347814984879e8b0db Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Mon, 13 May 2019 15:02:34 +0100
Subject: [PATCH 02/25] Fixed bug that prevented viewing of the command line
 options for the writedb_entry script, via the -help argument

---
 etc/writedb_entry | 5 ++++-
 1 file changed, 4 insertions(+), 1 deletion(-)

diff --git a/etc/writedb_entry b/etc/writedb_entry
index 5e899c26b..802ecd561 100755
--- a/etc/writedb_entry
+++ b/etc/writedb_entry
@@ -12,6 +12,7 @@
 
 QUIET=no
 PROXY_SETTINGS=
+HELP_FLAG=false
 
 add_proxy_properties() {
 
@@ -80,6 +81,8 @@ while test $# != 0
        DEFAULT_CONNECTION="$1" ;;
     -D*)
        FLAGS="$FLAGS $1" ;;
+    -help)
+       HELP_FLAG=true ;;
     *) break ;;
     esac
     shift
@@ -109,7 +112,7 @@ do
 done
 
 # Check that a database connection has been provided
-if [[ "$DEFAULT_CONNECTION" = "" ]]
+if [[ "$DEFAULT_CONNECTION" = "" ]] && [[ "$HELP_FLAG" != "true" ]]
 then 
 	echo "ERROR: Please supply a database connection argument"
 	exit 1

From 544c8c31aedac09e4763dadb1567f97b1e509a2f Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Mon, 13 May 2019 15:23:56 +0100
Subject: [PATCH 03/25] Removed an else clause that causes performance issues

---
 src/main/java/uk/ac/sanger/artemis/util/FastVector.java | 2 --
 1 file changed, 2 deletions(-)

diff --git a/src/main/java/uk/ac/sanger/artemis/util/FastVector.java b/src/main/java/uk/ac/sanger/artemis/util/FastVector.java
index d79dc474a..9b2cdd5ab 100644
--- a/src/main/java/uk/ac/sanger/artemis/util/FastVector.java
+++ b/src/main/java/uk/ac/sanger/artemis/util/FastVector.java
@@ -47,8 +47,6 @@ public boolean add(Object object)
   {
     if(object == null)
       throw new Error("internal error - adding a null object");
-    else if(contains(object))
-      throw new Error("internal error - object added a second time");
 
     return super.add(object);
   }

From 66d0250c69f4c27d5fd9fc8587005118db741149 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Mon, 13 May 2019 16:04:47 +0100
Subject: [PATCH 04/25] Added fix to prevent exceptions being thrown when
 selecting an indexed GFF contig that has no features

---
 .../variant/NoFeaturesException.java          | 23 +++++++
 .../components/variant/TabixReader.java       |  6 ++
 .../artemis/io/IndexedGFFDocumentEntry.java   | 16 ++++-
 .../io/IndexedGFFDocumentEntryTest.java       | 63 +++++++++++++++++++
 4 files changed, 107 insertions(+), 1 deletion(-)
 create mode 100644 src/main/java/uk/ac/sanger/artemis/components/variant/NoFeaturesException.java
 create mode 100644 src/test/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntryTest.java

diff --git a/src/main/java/uk/ac/sanger/artemis/components/variant/NoFeaturesException.java b/src/main/java/uk/ac/sanger/artemis/components/variant/NoFeaturesException.java
new file mode 100644
index 000000000..3ce8c6f71
--- /dev/null
+++ b/src/main/java/uk/ac/sanger/artemis/components/variant/NoFeaturesException.java
@@ -0,0 +1,23 @@
+package uk.ac.sanger.artemis.components.variant;
+
+/**
+ * An exception to be thrown when
+ * a contig that has no features
+ * is requested to be displayed.
+ * 
+ * @author kp11
+ *
+ */
+public class NoFeaturesException extends RuntimeException
+{
+	private static final long serialVersionUID = 1L;
+
+	/**
+	 * Constructor.
+	 * @param message - reason for exception
+	 */
+	public NoFeaturesException(String message)
+	{
+		super(message);
+	}	
+}
diff --git a/src/main/java/uk/ac/sanger/artemis/components/variant/TabixReader.java b/src/main/java/uk/ac/sanger/artemis/components/variant/TabixReader.java
index d9c91678e..52cc3d8d6 100644
--- a/src/main/java/uk/ac/sanger/artemis/components/variant/TabixReader.java
+++ b/src/main/java/uk/ac/sanger/artemis/components/variant/TabixReader.java
@@ -335,6 +335,12 @@ public String next() throws IOException {
 	};
 
 	public Iterator query(final int tid, final int beg, final int end) {
+		
+        if (tid == -1)
+        {
+        	throw new NoFeaturesException("The current contig has no features [missing index entry].");
+        }
+        
 		TPair64[] off, chunks;
 		long min_off;
 		TIndex idx = mIndex[tid];
diff --git a/src/main/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntry.java b/src/main/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntry.java
index 3546168ca..3fd07673b 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntry.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntry.java
@@ -44,6 +44,7 @@
 import uk.ac.sanger.artemis.components.FeatureDisplay;
 import uk.ac.sanger.artemis.components.genebuilder.GeneUtils;
 import uk.ac.sanger.artemis.components.variant.FeatureContigPredicate;
+import uk.ac.sanger.artemis.components.variant.NoFeaturesException;
 import uk.ac.sanger.artemis.components.variant.TabixReader;
 import uk.ac.sanger.artemis.util.CacheHashMap;
 import uk.ac.sanger.artemis.util.DatabaseDocument;
@@ -255,6 +256,10 @@ private void getFeaturesInRange(IndexContig c, Range range, FeatureVector featur
       tabixIterator = reader.query(r);
     }
     catch(NullPointerException npe){}
+    catch (NoFeaturesException e) 
+    {
+  	  // tabixIterator will be null
+    }
      
     if(tabixIterator == null)
       return;
@@ -890,12 +895,21 @@ public int getFeatureCount()
 
     featureCount = 0;
     List<IndexContig> contigs = getListOfContigs();
+    TabixReader.Iterator tabixIterator = null;
     for(IndexContig c: contigs)
     {
       int nfeatures = 0;
       final String r = c.chr+":"+1+"-"+Integer.MAX_VALUE;
 
-      TabixReader.Iterator tabixIterator = reader.query(r);
+      try 
+      {
+    	  tabixIterator = reader.query(r);
+      } 
+      catch (NoFeaturesException e) 
+      {
+    	  // tabixIterator will be null
+      }
+      
       if(tabixIterator == null)
         continue;
 
diff --git a/src/test/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntryTest.java b/src/test/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntryTest.java
new file mode 100644
index 000000000..57be1defa
--- /dev/null
+++ b/src/test/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntryTest.java
@@ -0,0 +1,63 @@
+/* 
+ * This file is part of Artemis
+ *
+ * Copyright (C) 2019  Genome Research Limited
+ *
+ * This program is free software; you can redistribute it and/or
+ * modify it under the terms of the GNU General Public License
+ * as published by the Free Software Foundation; either version 2
+ * of the License, or (at your option) any later version.
+ *
+ * This program is distributed in the hope that it will be useful,
+ * but WITHOUT ANY WARRANTY; without even the implied warranty of
+ * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+ * GNU General Public License for more details.
+ *
+ * You should have received a copy of the GNU General Public License
+ * along with this program; if not, write to the Free Software
+ * Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA  02111-1307, USA.
+ *
+ */
+package uk.ac.sanger.artemis.io;
+
+import static org.junit.Assert.fail;
+
+import java.io.IOException;
+import org.junit.Test;
+
+import uk.ac.sanger.artemis.components.variant.NoFeaturesException;
+import uk.ac.sanger.artemis.components.variant.TabixReader;
+
+/**
+ * Unit test for the IndexedGFFDocumentEntry class.
+ * It currently only covers new functionality/changes.
+ * 
+ * @author kp11
+ *
+ */
+public class IndexedGFFDocumentEntryTest
+{
+
+	public static final String TEST_INDEXED_GFF_FILE = "/data/gff/indexed-gff.gff.gz";
+	
+	/**
+	 * Test the query method.
+	 * @throws IOException 
+	 */
+	@Test
+	public void testQuery() throws IOException 
+	{	
+		TabixReader reader = new TabixReader(
+				IndexedGFFDocumentEntryTest.class.getResource(TEST_INDEXED_GFF_FILE).getFile());
+		
+		try 
+		{
+			reader.query(-1, 1, 19);
+			fail("Expected a NoFeaturesException to be thrown");
+		} 
+		catch (NoFeaturesException e)
+		{
+			// expected
+		}
+	}
+}

From 3511e3227d326bd346d38619c21db62e59027423 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Mon, 13 May 2019 16:05:56 +0100
Subject: [PATCH 05/25] Enabled writedb_entry script -h option

---
 etc/writedb_entry | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/etc/writedb_entry b/etc/writedb_entry
index 802ecd561..6c89af23c 100755
--- a/etc/writedb_entry
+++ b/etc/writedb_entry
@@ -83,6 +83,8 @@ while test $# != 0
        FLAGS="$FLAGS $1" ;;
     -help)
        HELP_FLAG=true ;;
+    -h)
+       HELP_FLAG=true ;;
     *) break ;;
     esac
     shift

From f2bd3effb43e5bb731b3305355432418af6e3476 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Mon, 13 May 2019 16:08:53 +0100
Subject: [PATCH 06/25] Changed some debug levels to prevent reams of log
 messages for large files

---
 etc/log4j.properties | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/etc/log4j.properties b/etc/log4j.properties
index 921fa7877..0ff647a61 100644
--- a/etc/log4j.properties
+++ b/etc/log4j.properties
@@ -41,7 +41,8 @@ log4j.logger.uk.ac.sanger.artemis.components.alignment.LookSeqPanel=DEBUG, R
 log4j.logger.uk.ac.sanger.artemis.components.alignment.BamView=INFO, R
 log4j.logger.uk.ac.sanger.artemis.components.variant=DEBUG, R
 log4j.logger.uk.ac.sanger.artemis.io.ReadAndWriteEntry=DEBUG, R
-log4j.logger.uk.ac.sanger.artemis.io.GFFStreamFeature=DEBUG, R
+log4j.logger.uk.ac.sanger.artemis.io.GFFStreamFeature=INFO, R
+log4j.logger.uk.ac.sanger.artemis.io.IndexedGFFDocumentEntry=INFO, R
 log4j.logger.uk.ac.sanger.artemis.util.FTPSeekableStream=DEBUG, R
 log4j.logger.uk.ac.sanger.artemis.plot.UserDataAlgorithm=DEBUG, R
 log4j.logger.uk.ac.sanger.artemis.ComparisonDataFactory=INFO, R

From ccc7a030f40a092533b5f30954f0dd2ae1ece8cf Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Mon, 13 May 2019 16:54:52 +0100
Subject: [PATCH 07/25] Reorganised test data

---
 .../uk/ac/sanger/artemis/FeatureTest.java     |     4 +-
 .../artemis/components/EditMenuTest.java      |     2 +-
 .../components/alignment/BamUtilsTest.java    |    12 +-
 .../components/alignment/MappedReadsTest.java |     4 +-
 .../MappedReadsWithMultipleFilesTest.java     |     2 +-
 .../components/variant/WriteVCFTest.java      |     8 +-
 .../variant/WriteVariantSitesTest.java        |    20 +-
 .../artemis/editor/EvidenceViewerTest.java    |     2 +-
 .../java/uk/ac/sanger/artemis/io/GFFTest.java |    17 +-
 .../ac/sanger/artemis/io/GffToEMBLTest.java   |     6 +-
 .../artemis/io/ValidateFeatureTest.java       |    20 +-
 .../ac/sanger/artemis/plot/UserPlotTest.java  |     6 +-
 .../resources/data/{ => alignment}/MAL_8h.bam |   Bin
 .../data/{ => alignment}/MAL_8h.bam.bai       |   Bin
 .../data/{ => alignment}/MAL_8h_noindex.bam   |   Bin
 .../resources/data/{ => alignment}/NV.bam     |   Bin
 .../resources/data/{ => alignment}/NV.bam.bai |   Bin
 .../resources/data/{ => alignment}/NV.cram    |   Bin
 .../data/{ => alignment}/NV.cram.crai         |   Bin
 src/test/resources/data/blast/blast_test.fa   |     5 +
 .../data/blast/blast_test_results.blast       |    46 +
 .../resources/data/{ => embl}/MAL1.embl.gz    |   Bin
 .../resources/data/{ => embl}/test.embl.gz    |   Bin
 src/test/resources/data/{ => fasta}/NV.fasta  |     0
 .../resources/data/{ => fasta}/NV.fasta.fai   |     0
 .../data/fasta/clostridium_cellulosi.fa.gz    |   Bin 0 -> 693590 bytes
 src/test/resources/data/fasta/test.fa.gz      |   Bin 0 -> 119 bytes
 .../data/genbank/SeaOtter_chrMT.gbk.gz        |   Bin 0 -> 9650 bytes
 .../data/{ => gff}/Pf3D7_01_02_v3.gff.gz      |   Bin
 src/test/resources/data/gff/indexed-gff.fa    | 26469 ++++++++++++++++
 .../resources/data/gff/indexed-gff.gff.gz     |   Bin 0 -> 30661 bytes
 .../resources/data/gff/indexed-gff.gff.gz.tbi |   Bin 0 -> 539 bytes
 src/test/resources/data/gff/notindexed.gff.gz |   Bin 0 -> 462841 bytes
 .../data/gff/notindexed_compare.gff.gz        |   Bin 0 -> 615919 bytes
 src/test/resources/data/{ => gff}/test.gff.gz |   Bin
 .../data/{ => gff}/test_boundary.gff.gz       |   Bin
 .../data/gtf/clostridium_cellulosi.gtf.gz     |   Bin 0 -> 137648 bytes
 .../data/{ => vcf}/MAL1_8_16_24h.raw.bcf      |   Bin
 .../data/{ => vcf}/MAL1_8_16_24h.raw.bcf.bci  |   Bin
 src/test/resources/data/{ => vcf}/test.vcf.gz |   Bin
 .../resources/data/{ => vcf}/test.vcf.gz.tbi  |   Bin
 .../resources/data/{ => vcf}/test1.vcf.gz     |   Bin
 .../resources/data/{ => vcf}/test1.vcf.gz.tbi |   Bin
 .../resources/data/{ => vcf}/test2.vcf.gz     |   Bin
 .../resources/data/{ => vcf}/test2.vcf.gz.tbi |   Bin
 .../resources/data/{ => vcf}/test3.vcf.gz     |   Bin
 .../resources/data/{ => vcf}/test3.vcf.gz.tbi |   Bin
 .../resources/data/{ => vcf}/test4.vcf.gz     |   Bin
 .../resources/data/{ => vcf}/test4.vcf.gz.tbi |   Bin
 49 files changed, 26570 insertions(+), 53 deletions(-)
 rename src/test/resources/data/{ => alignment}/MAL_8h.bam (100%)
 rename src/test/resources/data/{ => alignment}/MAL_8h.bam.bai (100%)
 rename src/test/resources/data/{ => alignment}/MAL_8h_noindex.bam (100%)
 rename src/test/resources/data/{ => alignment}/NV.bam (100%)
 rename src/test/resources/data/{ => alignment}/NV.bam.bai (100%)
 rename src/test/resources/data/{ => alignment}/NV.cram (100%)
 rename src/test/resources/data/{ => alignment}/NV.cram.crai (100%)
 create mode 100644 src/test/resources/data/blast/blast_test.fa
 create mode 100644 src/test/resources/data/blast/blast_test_results.blast
 rename src/test/resources/data/{ => embl}/MAL1.embl.gz (100%)
 rename src/test/resources/data/{ => embl}/test.embl.gz (100%)
 rename src/test/resources/data/{ => fasta}/NV.fasta (100%)
 rename src/test/resources/data/{ => fasta}/NV.fasta.fai (100%)
 create mode 100644 src/test/resources/data/fasta/clostridium_cellulosi.fa.gz
 create mode 100644 src/test/resources/data/fasta/test.fa.gz
 create mode 100644 src/test/resources/data/genbank/SeaOtter_chrMT.gbk.gz
 rename src/test/resources/data/{ => gff}/Pf3D7_01_02_v3.gff.gz (100%)
 create mode 100644 src/test/resources/data/gff/indexed-gff.fa
 create mode 100644 src/test/resources/data/gff/indexed-gff.gff.gz
 create mode 100644 src/test/resources/data/gff/indexed-gff.gff.gz.tbi
 create mode 100644 src/test/resources/data/gff/notindexed.gff.gz
 create mode 100644 src/test/resources/data/gff/notindexed_compare.gff.gz
 rename src/test/resources/data/{ => gff}/test.gff.gz (100%)
 rename src/test/resources/data/{ => gff}/test_boundary.gff.gz (100%)
 create mode 100644 src/test/resources/data/gtf/clostridium_cellulosi.gtf.gz
 rename src/test/resources/data/{ => vcf}/MAL1_8_16_24h.raw.bcf (100%)
 rename src/test/resources/data/{ => vcf}/MAL1_8_16_24h.raw.bcf.bci (100%)
 rename src/test/resources/data/{ => vcf}/test.vcf.gz (100%)
 rename src/test/resources/data/{ => vcf}/test.vcf.gz.tbi (100%)
 rename src/test/resources/data/{ => vcf}/test1.vcf.gz (100%)
 rename src/test/resources/data/{ => vcf}/test1.vcf.gz.tbi (100%)
 rename src/test/resources/data/{ => vcf}/test2.vcf.gz (100%)
 rename src/test/resources/data/{ => vcf}/test2.vcf.gz.tbi (100%)
 rename src/test/resources/data/{ => vcf}/test3.vcf.gz (100%)
 rename src/test/resources/data/{ => vcf}/test3.vcf.gz.tbi (100%)
 rename src/test/resources/data/{ => vcf}/test4.vcf.gz (100%)
 rename src/test/resources/data/{ => vcf}/test4.vcf.gz.tbi (100%)

diff --git a/src/test/java/uk/ac/sanger/artemis/FeatureTest.java b/src/test/java/uk/ac/sanger/artemis/FeatureTest.java
index a9bf04c37..5fc9de44f 100644
--- a/src/test/java/uk/ac/sanger/artemis/FeatureTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/FeatureTest.java
@@ -355,7 +355,7 @@ private void displaySegments(Feature f)
 	public void setup() 
 	{
 		DocumentEntryAutosaveThread.setDisabled(true);
-		egrp = Utils.getEntryGroup("/data/Pf3D7_01_02_v3.gff.gz");
+		egrp = Utils.getEntryGroup("/data/gff/Pf3D7_01_02_v3.gff.gz");
 
 	    features = egrp.getAllFeatures();
 	}
@@ -891,7 +891,7 @@ public void testHasValidEMBLKey() throws Exception
 	public void testHasRequiredQualifiers() throws Exception
 	{
 		//Feature f = Utils.getFeatureByIdAndKey(POLYPEPTIDE_FEATURE_ID, POLYPEPTIDE_ID, features);
-		/*egrp = Utils.getEntryGroup("/data/MAL1.embl.gz");
+		/*egrp = Utils.getEntryGroup("/data/embl/MAL1.embl.gz");
 	    features = egrp.getAllFeatures();
 	    
 		Feature f = Utils.getFeatureByIdAndKey("PFA0410w", CDS_FEATURE_ID, features);
diff --git a/src/test/java/uk/ac/sanger/artemis/components/EditMenuTest.java b/src/test/java/uk/ac/sanger/artemis/components/EditMenuTest.java
index 244ebef90..8801cdbf6 100644
--- a/src/test/java/uk/ac/sanger/artemis/components/EditMenuTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/components/EditMenuTest.java
@@ -51,7 +51,7 @@ public void gffUnMergeAndUndo()
     if(GraphicsEnvironment.getLocalGraphicsEnvironment().isHeadless())
       return;
         
-    final EntryGroup egrp = Utils.getEntryGroup("/data/Pf3D7_01_02_v3.gff.gz");
+    final EntryGroup egrp = Utils.getEntryGroup("/data/gff/Pf3D7_01_02_v3.gff.gz");
 
     FeatureVector features = egrp.getAllFeatures();
     final Selection selection = new Selection(null);
diff --git a/src/test/java/uk/ac/sanger/artemis/components/alignment/BamUtilsTest.java b/src/test/java/uk/ac/sanger/artemis/components/alignment/BamUtilsTest.java
index 31a4e73d7..60431ee2a 100644
--- a/src/test/java/uk/ac/sanger/artemis/components/alignment/BamUtilsTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/components/alignment/BamUtilsTest.java
@@ -125,14 +125,14 @@ public void testCreateIndexFileFromScratch() throws Exception
 		fileBai.deleteOnExit();
 		assertTrue(fileBai.exists());
 		assertTrue(fileBai.length()==0);
-		BamUtils.createIndexFileFromScratch(this.getClass().getResource("/data/MAL_8h.bam").getPath(), fileBai);
+		BamUtils.createIndexFileFromScratch(this.getClass().getResource("/data/alignment/MAL_8h.bam").getPath(), fileBai);
 		assertTrue(fileBai.length()>0);
 		
 		File fileCrai = File.createTempFile("unit_test_bam_index_file1", ".crai");
 		fileCrai.deleteOnExit();
 		assertTrue(fileCrai.exists());
 		assertTrue(fileCrai.length()==0);
-		BamUtils.createIndexFileFromScratch(this.getClass().getResource("/data/NV.cram").getPath(), fileCrai);
+		BamUtils.createIndexFileFromScratch(this.getClass().getResource("/data/alignment/NV.cram").getPath(), fileCrai);
 		assertTrue(fileCrai.length()>0);
 	}
 	
@@ -180,7 +180,7 @@ public void testGetIndexFileForFile() throws Exception
 		
 		// Check getting an already existing BAM index file
 		//
-		File alreadyExistingBamIndexFile = BamUtils.getIndexFile(this.getClass().getResource("/data/MAL_8h.bam").getPath());
+		File alreadyExistingBamIndexFile = BamUtils.getIndexFile(this.getClass().getResource("/data/alignment/MAL_8h.bam").getPath());
 		assertNotNull(alreadyExistingBamIndexFile);
 		assertTrue(alreadyExistingBamIndexFile.exists());
 		assertEquals("MAL_8h.bam.bai", alreadyExistingBamIndexFile.getName());
@@ -196,7 +196,7 @@ public void testGetIndexFileForFile() throws Exception
 		}
 		try 
 		{
-			File createdBamIndexFile = BamUtils.getIndexFile(this.getClass().getResource("/data/MAL_8h_noindex.bam").getPath());
+			File createdBamIndexFile = BamUtils.getIndexFile(this.getClass().getResource("/data/alignment/MAL_8h_noindex.bam").getPath());
 			assertNotNull(createdBamIndexFile);
 			assertTrue(createdBamIndexFile.exists());
 			assertEquals("MAL_8h_noindex.bam.bai", createdBamIndexFile.getName());
@@ -214,7 +214,7 @@ public void testGetIndexFileForFile() throws Exception
 		// Check getting an index file for a CRAM file with no index.
 		// Should create one.
 		//
-		File createdCramIndexFile = BamUtils.getIndexFile(this.getClass().getResource("/data/NV.cram").getPath());
+		File createdCramIndexFile = BamUtils.getIndexFile(this.getClass().getResource("/data/alignment/NV.cram").getPath());
 		assertNotNull(createdCramIndexFile);
 		assertTrue(createdCramIndexFile.exists());
 		assertEquals("NV.cram.crai", createdCramIndexFile.getName());
@@ -222,7 +222,7 @@ public void testGetIndexFileForFile() throws Exception
 				
 		// Check getting an already existing CRAM index file
 		//
-		File alreadyExistingCramIndexFile = BamUtils.getIndexFile(this.getClass().getResource("/data/NV.cram").getPath());
+		File alreadyExistingCramIndexFile = BamUtils.getIndexFile(this.getClass().getResource("/data/alignment/NV.cram").getPath());
 		assertNotNull(alreadyExistingCramIndexFile);
 		assertTrue(alreadyExistingCramIndexFile.exists());
 		assertEquals("NV.cram.crai", alreadyExistingCramIndexFile.getName());
diff --git a/src/test/java/uk/ac/sanger/artemis/components/alignment/MappedReadsTest.java b/src/test/java/uk/ac/sanger/artemis/components/alignment/MappedReadsTest.java
index d75d59215..ca3683aa2 100644
--- a/src/test/java/uk/ac/sanger/artemis/components/alignment/MappedReadsTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/components/alignment/MappedReadsTest.java
@@ -54,9 +54,9 @@ public static void setUp()
     // ignore if in headless mode with no x11
     if(GraphicsEnvironment.isHeadless())
       return;
-    URL entryFile = MappedReadsTest.class.getResource("/data/MAL_8h.bam");
+    URL entryFile = MappedReadsTest.class.getResource("/data/alignment/MAL_8h.bam");
     System.setProperty("bam", entryFile.getFile());
-    final EntryGroup egrp = Utils.getEntryGroup("/data/MAL1.embl.gz");
+    final EntryGroup egrp = Utils.getEntryGroup("/data/embl/MAL1.embl.gz");
     ee = new EntryEdit(egrp);
     ee.setVisible(true);
 
diff --git a/src/test/java/uk/ac/sanger/artemis/components/alignment/MappedReadsWithMultipleFilesTest.java b/src/test/java/uk/ac/sanger/artemis/components/alignment/MappedReadsWithMultipleFilesTest.java
index ec1313214..93e584324 100644
--- a/src/test/java/uk/ac/sanger/artemis/components/alignment/MappedReadsWithMultipleFilesTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/components/alignment/MappedReadsWithMultipleFilesTest.java
@@ -123,7 +123,7 @@ private void createBamView(boolean useBothFeatures, String ... bamFiles)
     
     System.setProperty("bam", buf.toString());
     
-    final EntryGroup egrp = Utils.getEntryGroup("/data/MAL1.embl.gz");
+    final EntryGroup egrp = Utils.getEntryGroup("/data/embl/MAL1.embl.gz");
     ee = new EntryEdit(egrp);
     ee.setVisible(true);
 
diff --git a/src/test/java/uk/ac/sanger/artemis/components/variant/WriteVCFTest.java b/src/test/java/uk/ac/sanger/artemis/components/variant/WriteVCFTest.java
index 5222ac9f0..2b352b85e 100644
--- a/src/test/java/uk/ac/sanger/artemis/components/variant/WriteVCFTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/components/variant/WriteVCFTest.java
@@ -535,8 +535,8 @@ private StringWriter getFeatureWriter(String ids, final VCFview view)
   public static void oneTimeSetUp()
   {
     // load VCF
-    URL ref = WriteVCFTest.class.getResource("/data/test.embl.gz");
-    URL vcf = WriteVCFTest.class.getResource("/data/test.vcf.gz");
+    URL ref = WriteVCFTest.class.getResource("/data/embl/test.embl.gz");
+    URL vcf = WriteVCFTest.class.getResource("/data/vcf/test.vcf.gz");
   
     List<String> vcfFileList = new Vector<String>();
     vcfFileList.add(vcf.getFile());
@@ -547,8 +547,8 @@ public static void oneTimeSetUp()
         ref.getFile(), null, null);
     
     // load BCF
-    ref = WriteVCFTest.class.getResource("/data/MAL1.embl.gz");
-    vcf = WriteVCFTest.class.getResource("/data/MAL1_8_16_24h.raw.bcf");
+    ref = WriteVCFTest.class.getResource("/data/embl/MAL1.embl.gz");
+    vcf = WriteVCFTest.class.getResource("/data/vcf/MAL1_8_16_24h.raw.bcf");
     
     List<String> bcfFileList = new Vector<String>();
     bcfFileList.add(vcf.getFile());
diff --git a/src/test/java/uk/ac/sanger/artemis/components/variant/WriteVariantSitesTest.java b/src/test/java/uk/ac/sanger/artemis/components/variant/WriteVariantSitesTest.java
index 447e669ca..dedf179fe 100644
--- a/src/test/java/uk/ac/sanger/artemis/components/variant/WriteVariantSitesTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/components/variant/WriteVariantSitesTest.java
@@ -30,8 +30,8 @@ public class WriteVariantSitesTest
   public void testSNPSites()
   {
     // load VCF
-    final URL ref = WriteVCFTest.class.getResource("/data/test.embl.gz");
-    final URL vcf = WriteVCFTest.class.getResource("/data/test1.vcf.gz");
+    final URL ref = WriteVCFTest.class.getResource("/data/embl/test.embl.gz");
+    final URL vcf = WriteVCFTest.class.getResource("/data/vcf/test1.vcf.gz");
     VCFview vcfView = getVcfView(ref, vcf);
     
     StringWriter sw = new StringWriter();
@@ -59,8 +59,8 @@ public void testSNPSites()
   public void testInsertionSites()
   {
     // load VCF
-    final URL ref = WriteVCFTest.class.getResource("/data/test.embl.gz");
-    final URL vcf = WriteVCFTest.class.getResource("/data/test2.vcf.gz");
+    final URL ref = WriteVCFTest.class.getResource("/data/embl/test.embl.gz");
+    final URL vcf = WriteVCFTest.class.getResource("/data/vcf/test2.vcf.gz");
     VCFview vcfView = getVcfView(ref, vcf);
     
     StringWriter sw = new StringWriter();
@@ -88,8 +88,8 @@ public void testInsertionSites()
   public void testMultiAlleleSites()
   {
     // load VCF
-    final URL ref = WriteVCFTest.class.getResource("/data/test.embl.gz");
-    final URL vcf = WriteVCFTest.class.getResource("/data/test3.vcf.gz");
+    final URL ref = WriteVCFTest.class.getResource("/data/embl/test.embl.gz");
+    final URL vcf = WriteVCFTest.class.getResource("/data/vcf/test3.vcf.gz");
     VCFview vcfView = getVcfView(ref, vcf);
     
     StringWriter sw = new StringWriter();
@@ -118,8 +118,8 @@ public void testMultiAlleleSites()
   public void testDeletionSites()
   {
     // load VCF
-    final URL ref = WriteVCFTest.class.getResource("/data/test.embl.gz");
-    final URL vcf = WriteVCFTest.class.getResource("/data/test4.vcf.gz");
+    final URL ref = WriteVCFTest.class.getResource("/data/embl/test.embl.gz");
+    final URL vcf = WriteVCFTest.class.getResource("/data/vcf/test4.vcf.gz");
     VCFview vcfView = getVcfView(ref, vcf);
     
     StringWriter sw = new StringWriter();
@@ -153,8 +153,8 @@ private VCFview getVcfView(final URL ref, final URL vcf)
         ref.getFile(), null, null);
     
     // load BCF
-/*    ref = WriteVCFTest.class.getResource("/data/MAL1.embl.gz");
-    vcf = WriteVCFTest.class.getResource("/data/MAL1_8_16_24h.raw.bcf");
+/*    ref = WriteVCFTest.class.getResource("/data/embl/MAL1.embl.gz");
+    vcf = WriteVCFTest.class.getResource("/data/vcf/MAL1_8_16_24h.raw.bcf");
     
     List<String> bcfFileList = new Vector<String>();
     bcfFileList.add(vcf.getFile());
diff --git a/src/test/java/uk/ac/sanger/artemis/editor/EvidenceViewerTest.java b/src/test/java/uk/ac/sanger/artemis/editor/EvidenceViewerTest.java
index a84942ed1..3649ef773 100644
--- a/src/test/java/uk/ac/sanger/artemis/editor/EvidenceViewerTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/editor/EvidenceViewerTest.java
@@ -37,7 +37,7 @@ public void testSetPfamUrl()
 	    
 	    // Given
 	    
-	    final EntryGroup egrp = Utils.getEntryGroup("/data/Pf3D7_01_02_v3.gff.gz");
+	    final EntryGroup egrp = Utils.getEntryGroup("/data/gff/Pf3D7_01_02_v3.gff.gz");
 
 	    FeatureVector features = egrp.getAllFeatures();
 	    
diff --git a/src/test/java/uk/ac/sanger/artemis/io/GFFTest.java b/src/test/java/uk/ac/sanger/artemis/io/GFFTest.java
index 6d3c99dca..6d8235695 100644
--- a/src/test/java/uk/ac/sanger/artemis/io/GFFTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/io/GFFTest.java
@@ -21,19 +21,16 @@
 package uk.ac.sanger.artemis.io;
 
 import static org.junit.Assert.assertTrue;
+import static org.junit.Assert.fail;
 import static org.junit.Assert.assertEquals;
-import junit.framework.Assert;
 
 import org.junit.Test;
 
 import uk.ac.sanger.artemis.EntryGroup;
-import uk.ac.sanger.artemis.Options;
 import uk.ac.sanger.artemis.SimpleEntryGroup;
-import uk.ac.sanger.artemis.components.genebuilder.GeneUtils;
 
 import uk.ac.sanger.artemis.sequence.NoSequenceException;
 import uk.ac.sanger.artemis.util.OutOfRangeException;
-import uk.ac.sanger.artemis.util.StringVector;
 
 public class GFFTest
 {
@@ -46,7 +43,7 @@ public void testIdAfterLocationChange()
   {
     try
     {
-      final Entry entry = Utils.getEntry("/data/test_boundary.gff.gz");
+      final Entry entry = Utils.getEntry("/data/gff/test_boundary.gff.gz");
       Feature f = Utils.getCDSFeatureByIdPrefix("PF3D7_0200200.1", entry.getAllFeatures());
       Location oldLocation = f.getLocation();
       Location newLocation = new Location("join(25234..29035,29837..31168)");
@@ -59,7 +56,7 @@ public void testIdAfterLocationChange()
     }
     catch (Exception e)
     {
-      Assert.fail(e.getMessage());
+      fail(e.getMessage());
     }
   }
   
@@ -72,7 +69,7 @@ public void testGFFMultipleFastaOffset()
   {
     try
     {
-      final Entry entry = Utils.getEntry("/data/Pf3D7_01_02_v3.gff.gz");
+      final Entry entry = Utils.getEntry("/data/gff/Pf3D7_01_02_v3.gff.gz");
       final EntryGroup egrp = new SimpleEntryGroup();
       egrp.add(new uk.ac.sanger.artemis.Entry(entry));
       final uk.ac.sanger.artemis.FeatureVector features = egrp.getAllFeatures();
@@ -104,7 +101,7 @@ public void testGFFMultipleFastaOffset()
         }
         catch(InvalidRelationException e)
         {
-          Assert.fail(e.getMessage());
+          fail(e.getMessage());
         }
       }
       
@@ -112,11 +109,11 @@ public void testGFFMultipleFastaOffset()
     }
     catch (OutOfRangeException e)
     {
-      Assert.fail(e.getMessage());
+      fail(e.getMessage());
     }
     catch (NoSequenceException e)
     {
-      Assert.fail(e.getMessage());
+      fail(e.getMessage());
     }
   }
 }
diff --git a/src/test/java/uk/ac/sanger/artemis/io/GffToEMBLTest.java b/src/test/java/uk/ac/sanger/artemis/io/GffToEMBLTest.java
index 0ea4a2a71..2be1d48cc 100644
--- a/src/test/java/uk/ac/sanger/artemis/io/GffToEMBLTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/io/GffToEMBLTest.java
@@ -38,9 +38,9 @@ public class GffToEMBLTest
    */
   public void testGffToEMBL()
   {
-    testGffConversion("/data/Pf3D7_01_02_v3.gff.gz");
-    testGffConversion("/data/test.gff.gz");
-    testGffConversion("/data/test_boundary.gff.gz");
+    testGffConversion("/data/gff/Pf3D7_01_02_v3.gff.gz");
+    testGffConversion("/data/gff/test.gff.gz");
+    testGffConversion("/data/gff/test_boundary.gff.gz");
   }
   
   private void testGffConversion(String gff)
diff --git a/src/test/java/uk/ac/sanger/artemis/io/ValidateFeatureTest.java b/src/test/java/uk/ac/sanger/artemis/io/ValidateFeatureTest.java
index 7082c976f..d0ab83112 100644
--- a/src/test/java/uk/ac/sanger/artemis/io/ValidateFeatureTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/io/ValidateFeatureTest.java
@@ -57,7 +57,7 @@ public void setUp() throws Exception {
   @Test
   public void testGFF()
   {
-    testAll(Utils.getEntryGroup("/data/test.gff.gz"));
+    testAll(Utils.getEntryGroup("/data/gff/test.gff.gz"));
   }
   
   public static void testAll(final EntryGroup egrp)
@@ -97,7 +97,7 @@ public static void testAll(final EntryGroup egrp)
   @Test
   public void testGFFBoundary()
   {
-    final Entry entry = Utils.getEntry("/data/test_boundary.gff.gz");
+    final Entry entry = Utils.getEntry("/data/gff/test_boundary.gff.gz");
     final FeatureVector features = entry.getAllFeatures();
 
     for(uk.ac.sanger.artemis.io.Feature f: features)
@@ -120,7 +120,7 @@ public void testGFFBoundary()
   @Test
   public void testGFFStrand()
   {
-    final Entry entry = Utils.getEntry("/data/test_boundary.gff.gz");
+    final Entry entry = Utils.getEntry("/data/gff/test_boundary.gff.gz");
     final FeatureVector features = entry.getAllFeatures();
 
     for(uk.ac.sanger.artemis.io.Feature f: features)
@@ -143,7 +143,7 @@ public void testGFFStrand()
   @Test
   public void testGFFPhase()
   {
-    final Entry entry = Utils.getEntry("/data/test_boundary.gff.gz");
+    final Entry entry = Utils.getEntry("/data/gff/test_boundary.gff.gz");
     final FeatureVector features = entry.getAllFeatures();
 
     for(uk.ac.sanger.artemis.io.Feature f: features)
@@ -166,7 +166,7 @@ public void testGFFPhase()
   @Test
   public void testGFFCompleteGeneModel()
   {
-    final Entry entry = Utils.getEntry("/data/test_boundary.gff.gz");
+    final Entry entry = Utils.getEntry("/data/gff/test_boundary.gff.gz");
     final FeatureVector features = entry.getAllFeatures();
 
     for(uk.ac.sanger.artemis.io.Feature f: features)
@@ -195,7 +195,7 @@ public void testGFFCompleteGeneModel()
   @Test
   public void testGFFId()
   {
-    final Entry entry = Utils.getEntry("/data/test_boundary.gff.gz");
+    final Entry entry = Utils.getEntry("/data/gff/test_boundary.gff.gz");
     final FeatureVector features = entry.getAllFeatures();
 
     for(uk.ac.sanger.artemis.io.Feature f: features)
@@ -220,7 +220,7 @@ public void testGFFId()
   @Test
   public void testGFFPartials()
   {
-    final Entry entry = Utils.getEntry("/data/test_boundary.gff.gz");
+    final Entry entry = Utils.getEntry("/data/gff/test_boundary.gff.gz");
     final FeatureVector features = entry.getAllFeatures();
 
     for(uk.ac.sanger.artemis.io.Feature f: features)
@@ -247,7 +247,7 @@ public void testGFFPartials()
   @Test
   public void testGFFAttributes()
   {
-    final Entry entry = Utils.getEntry("/data/test_boundary.gff.gz");
+    final Entry entry = Utils.getEntry("/data/gff/test_boundary.gff.gz");
     final FeatureVector features = entry.getAllFeatures();
 
     for(uk.ac.sanger.artemis.io.Feature f: features)
@@ -274,7 +274,7 @@ public void testGFFValidStop()
   {
     try
     {
-      final Entry entry = Utils.getEntry("/data/test_boundary.gff.gz");
+      final Entry entry = Utils.getEntry("/data/gff/test_boundary.gff.gz");
       final FeatureVector features = entry.getAllFeatures();
       final EntryGroup egrp = new SimpleEntryGroup();
       egrp.add(new uk.ac.sanger.artemis.Entry(entry));
@@ -309,7 +309,7 @@ public void testGFFInternalStop()
   {
     try
     {
-      final Entry entry = Utils.getEntry("/data/test_boundary.gff.gz");
+      final Entry entry = Utils.getEntry("/data/gff/test_boundary.gff.gz");
       final FeatureVector features = entry.getAllFeatures();
       final EntryGroup egrp = new SimpleEntryGroup();
       egrp.add(new uk.ac.sanger.artemis.Entry(entry));
diff --git a/src/test/java/uk/ac/sanger/artemis/plot/UserPlotTest.java b/src/test/java/uk/ac/sanger/artemis/plot/UserPlotTest.java
index 461b4817b..5b84eb056 100644
--- a/src/test/java/uk/ac/sanger/artemis/plot/UserPlotTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/plot/UserPlotTest.java
@@ -46,7 +46,7 @@ public class UserPlotTest
   public void basePosition()
   {
     URL gffURL = UserPlotTest.class.getResource("/data/plot/base_position.plot.gz");
-    EntryGroup entryGrp = Utils.getEntryGroup("/data/MAL1.embl.gz");
+    EntryGroup entryGrp = Utils.getEntryGroup("/data/embl/MAL1.embl.gz");
     final Strand fwdStrand = entryGrp.getBases ().getForwardStrand ();
     final FileDocument doc = new FileDocument (new File(gffURL.getFile()));
     
@@ -72,7 +72,7 @@ public void basePosition()
   public void labelBasePosition()
   {
     URL gffURL = UserPlotTest.class.getResource("/data/plot/base_position_labels.plot.gz");
-    EntryGroup entryGrp = Utils.getEntryGroup("/data/MAL1.embl.gz");
+    EntryGroup entryGrp = Utils.getEntryGroup("/data/embl/MAL1.embl.gz");
     final Strand fwdStrand = entryGrp.getBases ().getForwardStrand ();
     final FileDocument doc = new FileDocument (new File(gffURL.getFile()));
     
@@ -102,7 +102,7 @@ public void labelBasePosition()
   public void indexed()
   {
     URL gffURL = UserPlotTest.class.getResource("/data/plot/index_tab_sorted.plot.gz");
-    EntryGroup entryGrp = Utils.getEntryGroup("/data/MAL1.embl.gz");
+    EntryGroup entryGrp = Utils.getEntryGroup("/data/embl/MAL1.embl.gz");
     final Strand fwdStrand = entryGrp.getBases ().getForwardStrand ();
     final FileDocument doc = new FileDocument (new File(gffURL.getFile()));
     try
diff --git a/src/test/resources/data/MAL_8h.bam b/src/test/resources/data/alignment/MAL_8h.bam
similarity index 100%
rename from src/test/resources/data/MAL_8h.bam
rename to src/test/resources/data/alignment/MAL_8h.bam
diff --git a/src/test/resources/data/MAL_8h.bam.bai b/src/test/resources/data/alignment/MAL_8h.bam.bai
similarity index 100%
rename from src/test/resources/data/MAL_8h.bam.bai
rename to src/test/resources/data/alignment/MAL_8h.bam.bai
diff --git a/src/test/resources/data/MAL_8h_noindex.bam b/src/test/resources/data/alignment/MAL_8h_noindex.bam
similarity index 100%
rename from src/test/resources/data/MAL_8h_noindex.bam
rename to src/test/resources/data/alignment/MAL_8h_noindex.bam
diff --git a/src/test/resources/data/NV.bam b/src/test/resources/data/alignment/NV.bam
similarity index 100%
rename from src/test/resources/data/NV.bam
rename to src/test/resources/data/alignment/NV.bam
diff --git a/src/test/resources/data/NV.bam.bai b/src/test/resources/data/alignment/NV.bam.bai
similarity index 100%
rename from src/test/resources/data/NV.bam.bai
rename to src/test/resources/data/alignment/NV.bam.bai
diff --git a/src/test/resources/data/NV.cram b/src/test/resources/data/alignment/NV.cram
similarity index 100%
rename from src/test/resources/data/NV.cram
rename to src/test/resources/data/alignment/NV.cram
diff --git a/src/test/resources/data/NV.cram.crai b/src/test/resources/data/alignment/NV.cram.crai
similarity index 100%
rename from src/test/resources/data/NV.cram.crai
rename to src/test/resources/data/alignment/NV.cram.crai
diff --git a/src/test/resources/data/blast/blast_test.fa b/src/test/resources/data/blast/blast_test.fa
new file mode 100644
index 000000000..ee7bd33bf
--- /dev/null
+++ b/src/test/resources/data/blast/blast_test.fa
@@ -0,0 +1,5 @@
+>100
+AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGCCCCGCAAGGGGAGCGGCAGACGGGTGAGTAACGCGTGGGAATCTACCGTGCCCTACGGTTGGGCCGTGTCTCAGTCCCAATGTGGCTGATCATCCTCTCAGACCAGCTATGGATCGTCGCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATCCAAC
+>200
+AACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGAGACCTTCGGGTCTAGTGGCGCACGGGTGCGTAACGCGTGGGAACCTGCCTTTAGGTTCCTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAAAGATCGTCGCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATCTTAC
+
diff --git a/src/test/resources/data/blast/blast_test_results.blast b/src/test/resources/data/blast/blast_test_results.blast
new file mode 100644
index 000000000..89e86aef2
--- /dev/null
+++ b/src/test/resources/data/blast/blast_test_results.blast
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diff --git a/src/test/resources/data/MAL1.embl.gz b/src/test/resources/data/embl/MAL1.embl.gz
similarity index 100%
rename from src/test/resources/data/MAL1.embl.gz
rename to src/test/resources/data/embl/MAL1.embl.gz
diff --git a/src/test/resources/data/test.embl.gz b/src/test/resources/data/embl/test.embl.gz
similarity index 100%
rename from src/test/resources/data/test.embl.gz
rename to src/test/resources/data/embl/test.embl.gz
diff --git a/src/test/resources/data/NV.fasta b/src/test/resources/data/fasta/NV.fasta
similarity index 100%
rename from src/test/resources/data/NV.fasta
rename to src/test/resources/data/fasta/NV.fasta
diff --git a/src/test/resources/data/NV.fasta.fai b/src/test/resources/data/fasta/NV.fasta.fai
similarity index 100%
rename from src/test/resources/data/NV.fasta.fai
rename to src/test/resources/data/fasta/NV.fasta.fai
diff --git a/src/test/resources/data/fasta/clostridium_cellulosi.fa.gz b/src/test/resources/data/fasta/clostridium_cellulosi.fa.gz
new file mode 100644
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diff --git a/src/test/resources/data/Pf3D7_01_02_v3.gff.gz b/src/test/resources/data/gff/Pf3D7_01_02_v3.gff.gz
similarity index 100%
rename from src/test/resources/data/Pf3D7_01_02_v3.gff.gz
rename to src/test/resources/data/gff/Pf3D7_01_02_v3.gff.gz
diff --git a/src/test/resources/data/gff/indexed-gff.fa b/src/test/resources/data/gff/indexed-gff.fa
new file mode 100644
index 000000000..04d6a8f57
--- /dev/null
+++ b/src/test/resources/data/gff/indexed-gff.fa
@@ -0,0 +1,26469 @@
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diff --git a/src/test/resources/data/gff/indexed-gff.gff.gz b/src/test/resources/data/gff/indexed-gff.gff.gz
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diff --git a/src/test/resources/data/gff/indexed-gff.gff.gz.tbi b/src/test/resources/data/gff/indexed-gff.gff.gz.tbi
new file mode 100644
index 0000000000000000000000000000000000000000..f310b811d8e60f7b5a93f6ad4c8b4267d7949cb5
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diff --git a/src/test/resources/data/test.gff.gz b/src/test/resources/data/gff/test.gff.gz
similarity index 100%
rename from src/test/resources/data/test.gff.gz
rename to src/test/resources/data/gff/test.gff.gz
diff --git a/src/test/resources/data/test_boundary.gff.gz b/src/test/resources/data/gff/test_boundary.gff.gz
similarity index 100%
rename from src/test/resources/data/test_boundary.gff.gz
rename to src/test/resources/data/gff/test_boundary.gff.gz
diff --git a/src/test/resources/data/gtf/clostridium_cellulosi.gtf.gz b/src/test/resources/data/gtf/clostridium_cellulosi.gtf.gz
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literal 0
HcmV?d00001

diff --git a/src/test/resources/data/MAL1_8_16_24h.raw.bcf b/src/test/resources/data/vcf/MAL1_8_16_24h.raw.bcf
similarity index 100%
rename from src/test/resources/data/MAL1_8_16_24h.raw.bcf
rename to src/test/resources/data/vcf/MAL1_8_16_24h.raw.bcf
diff --git a/src/test/resources/data/MAL1_8_16_24h.raw.bcf.bci b/src/test/resources/data/vcf/MAL1_8_16_24h.raw.bcf.bci
similarity index 100%
rename from src/test/resources/data/MAL1_8_16_24h.raw.bcf.bci
rename to src/test/resources/data/vcf/MAL1_8_16_24h.raw.bcf.bci
diff --git a/src/test/resources/data/test.vcf.gz b/src/test/resources/data/vcf/test.vcf.gz
similarity index 100%
rename from src/test/resources/data/test.vcf.gz
rename to src/test/resources/data/vcf/test.vcf.gz
diff --git a/src/test/resources/data/test.vcf.gz.tbi b/src/test/resources/data/vcf/test.vcf.gz.tbi
similarity index 100%
rename from src/test/resources/data/test.vcf.gz.tbi
rename to src/test/resources/data/vcf/test.vcf.gz.tbi
diff --git a/src/test/resources/data/test1.vcf.gz b/src/test/resources/data/vcf/test1.vcf.gz
similarity index 100%
rename from src/test/resources/data/test1.vcf.gz
rename to src/test/resources/data/vcf/test1.vcf.gz
diff --git a/src/test/resources/data/test1.vcf.gz.tbi b/src/test/resources/data/vcf/test1.vcf.gz.tbi
similarity index 100%
rename from src/test/resources/data/test1.vcf.gz.tbi
rename to src/test/resources/data/vcf/test1.vcf.gz.tbi
diff --git a/src/test/resources/data/test2.vcf.gz b/src/test/resources/data/vcf/test2.vcf.gz
similarity index 100%
rename from src/test/resources/data/test2.vcf.gz
rename to src/test/resources/data/vcf/test2.vcf.gz
diff --git a/src/test/resources/data/test2.vcf.gz.tbi b/src/test/resources/data/vcf/test2.vcf.gz.tbi
similarity index 100%
rename from src/test/resources/data/test2.vcf.gz.tbi
rename to src/test/resources/data/vcf/test2.vcf.gz.tbi
diff --git a/src/test/resources/data/test3.vcf.gz b/src/test/resources/data/vcf/test3.vcf.gz
similarity index 100%
rename from src/test/resources/data/test3.vcf.gz
rename to src/test/resources/data/vcf/test3.vcf.gz
diff --git a/src/test/resources/data/test3.vcf.gz.tbi b/src/test/resources/data/vcf/test3.vcf.gz.tbi
similarity index 100%
rename from src/test/resources/data/test3.vcf.gz.tbi
rename to src/test/resources/data/vcf/test3.vcf.gz.tbi
diff --git a/src/test/resources/data/test4.vcf.gz b/src/test/resources/data/vcf/test4.vcf.gz
similarity index 100%
rename from src/test/resources/data/test4.vcf.gz
rename to src/test/resources/data/vcf/test4.vcf.gz
diff --git a/src/test/resources/data/test4.vcf.gz.tbi b/src/test/resources/data/vcf/test4.vcf.gz.tbi
similarity index 100%
rename from src/test/resources/data/test4.vcf.gz.tbi
rename to src/test/resources/data/vcf/test4.vcf.gz.tbi

From 13ff32ba0b687a841a5d7ae6e27832e0fe439ac9 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Mon, 13 May 2019 17:06:55 +0100
Subject: [PATCH 08/25] Small header/javadoc changes

---
 src/main/java/uk/ac/sanger/artemis/io/FeatureTable.java | 8 +++-----
 1 file changed, 3 insertions(+), 5 deletions(-)

diff --git a/src/main/java/uk/ac/sanger/artemis/io/FeatureTable.java b/src/main/java/uk/ac/sanger/artemis/io/FeatureTable.java
index a4fd8758b..6684aff60 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/FeatureTable.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/FeatureTable.java
@@ -4,7 +4,7 @@
  *
  * This file is part of Artemis
  * 
- * Copyright (C) 1998,1999,2000  Genome Research Limited
+ * Copyright (C) 2019  Genome Research Limited
  * 
  * This program is free software; you can redistribute it and/or
  * modify it under the terms of the GNU General Public License
@@ -19,8 +19,7 @@
  * You should have received a copy of the GNU General Public License
  * along with this program; if not, write to the Free Software
  * Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA  02111-1307, USA.
- *
- * $Header: //tmp/pathsoft/artemis/uk/ac/sanger/artemis/io/FeatureTable.java,v 1.2 2009-06-01 09:45:32 tjc Exp $
+ * 
  */
 
 package uk.ac.sanger.artemis.io;
@@ -29,7 +28,6 @@
  *  This object contains all the features in an entry.
  *
  *  @author Kim Rutherford
- *  @version $Id: FeatureTable.java,v 1.2 2009-06-01 09:45:32 tjc Exp $
  */
 
 abstract class FeatureTable extends LineGroup {
@@ -51,7 +49,7 @@ private FeatureTree getFeatures () {
   }
 
   /**
-   *  Return a count of the number of Feature obejcts in this FeatureTable.
+   *  Return a count of the number of Feature objects in this FeatureTable.
    **/
   int getFeatureCount () {
     return getFeatures ().size ();

From 0523c64cf91dd9748f8e7fdc1018075a13b45799 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Mon, 13 May 2019 17:08:34 +0100
Subject: [PATCH 09/25] Added flag to assist with testing and removed Java 1.5
 check

---
 .../artemis/io/DocumentEntryFactory.java      | 48 +++++++++++++++----
 1 file changed, 38 insertions(+), 10 deletions(-)

diff --git a/src/main/java/uk/ac/sanger/artemis/io/DocumentEntryFactory.java b/src/main/java/uk/ac/sanger/artemis/io/DocumentEntryFactory.java
index 5a3f28b37..fcaa4c2e6 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/DocumentEntryFactory.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/DocumentEntryFactory.java
@@ -61,6 +61,12 @@ abstract public class DocumentEntryFactory
   final public static int GFF_FORMAT = 3;
 
   public static boolean REMOVE_PRODUCT_FROM_PSEUDOGENE = false;
+  
+  /** 
+   * Boolean indicating whether or not the user should 
+   * be asked if they want to use a GFF index. 
+   */
+  private static boolean displayIndexingQuestionForGffs = true;
 
 
   /**
@@ -77,19 +83,23 @@ public static DocumentEntry makeDocumentEntry (final EntryInformation entry_info
                                                  final ReadListener listener)
       throws IOException, EntryInformationException 
   {
-    if(!System.getProperty("java.version").startsWith("1.5.") &&
-        document.getInputStream() instanceof htsjdk.samtools.util.BlockCompressedInputStream)
+    if(document.getInputStream() instanceof htsjdk.samtools.util.BlockCompressedInputStream)
     {
       if(IndexedGFFDocumentEntry.isIndexed( ((File)document.getLocation()) ))
       {
-        Object[] possibleValues = { "Use index", "Ignore index" };
-        int sel = JOptionPane.showOptionDialog(null, 
-            "Use the GFF index file (to increase the performance)\n"+ 
-            "or concatenate the sequences together?",
-            "Indexed GFF", 
-            JOptionPane.DEFAULT_OPTION, JOptionPane.WARNING_MESSAGE,
-            null, possibleValues, possibleValues[0]);
-
+    	int sel = 0;
+    	
+    	if (getDisplayIndexingQuestionForGffs())
+    	{
+          Object[] possibleValues = { "Use index", "Ignore index" };
+          sel = JOptionPane.showOptionDialog(null, 
+              "Use the GFF index file (to increase the performance)\n"+ 
+              "or concatenate the sequences together?",
+              "Indexed GFF", 
+              JOptionPane.DEFAULT_OPTION, JOptionPane.WARNING_MESSAGE,
+              null, possibleValues, possibleValues[0]);
+    	}
+    	
         if(sel == 0)
           return new IndexedGFFDocumentEntry(document);
       }
@@ -237,4 +247,22 @@ private static void removeProductFromPseudogene(final DocumentEntry entry,
       }
     }
   }
+
+  /**
+   * Get the value of the displayIndexingQuestionForGffs property.
+   * @return boolean
+   */
+  public static boolean getDisplayIndexingQuestionForGffs()
+  {
+	return displayIndexingQuestionForGffs;
+  }
+
+  /**
+   * Set the value of the displayIndexingQuestionForGffs property.
+   * @param displayIndexingQuestionForGffs boolean
+   */
+  public static void setDisplayIndexingQuestionForGffs(boolean displayIndexingQuestionForGffs)
+  {
+	DocumentEntryFactory.displayIndexingQuestionForGffs = displayIndexingQuestionForGffs;
+  }
 }

From f65efe88005f1090aebbcb7d2d91ef602c7a01a0 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Wed, 15 May 2019 15:34:17 +0100
Subject: [PATCH 10/25] Performance improvement for the line group list

---
 .../uk/ac/sanger/artemis/io/LineGroup.java    |   12 +
 .../ac/sanger/artemis/io/LineGroupCache.java  |  272 ++++
 .../artemis/io/LineGroupIdGenerator.java      |   28 +
 .../artemis/io/SimpleDocumentEntry.java       |  233 +---
 .../sanger/artemis/io/LineGroupCacheTest.java |  461 +++++++
 .../artemis/io/LineGroupIdGeneratorTest.java  |   48 +
 .../artemis/io/SimpleDocumentEntryTest.java   | 1208 +++++++++++++++++
 7 files changed, 2063 insertions(+), 199 deletions(-)
 create mode 100644 src/main/java/uk/ac/sanger/artemis/io/LineGroupCache.java
 create mode 100644 src/main/java/uk/ac/sanger/artemis/io/LineGroupIdGenerator.java
 create mode 100644 src/test/java/uk/ac/sanger/artemis/io/LineGroupCacheTest.java
 create mode 100644 src/test/java/uk/ac/sanger/artemis/io/LineGroupIdGeneratorTest.java
 create mode 100644 src/test/java/uk/ac/sanger/artemis/io/SimpleDocumentEntryTest.java

diff --git a/src/main/java/uk/ac/sanger/artemis/io/LineGroup.java b/src/main/java/uk/ac/sanger/artemis/io/LineGroup.java
index 81f1c5159..9057d25f6 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/LineGroup.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/LineGroup.java
@@ -155,6 +155,9 @@ public abstract class LineGroup
     genbank_hash.put ("DBSOURCE","DBSOURCE");
     genbank_hash.put ("CONTIG","CONTIG");
   }
+  
+  /** A unique ID for this line group */
+  private Long uniqueId = LineGroupIdGenerator.createID();
 
   /**
    *  Try to read and return a new LineGroup object from a stream.
@@ -468,4 +471,13 @@ public static void writeStartOfGFFEntry (Writer writer) throws IOException
   public abstract void writeToStream (final Writer out_stream)
       throws IOException;
 
+  /**
+   * Return the line group's unique ID.
+   * @return Long
+   */
+  public Long getUniqueId()
+  {
+	return uniqueId;
+  }
+
 }
diff --git a/src/main/java/uk/ac/sanger/artemis/io/LineGroupCache.java b/src/main/java/uk/ac/sanger/artemis/io/LineGroupCache.java
new file mode 100644
index 000000000..755cdceaf
--- /dev/null
+++ b/src/main/java/uk/ac/sanger/artemis/io/LineGroupCache.java
@@ -0,0 +1,272 @@
+package uk.ac.sanger.artemis.io;
+
+import java.io.IOException;
+import java.io.Writer;
+import java.util.ArrayList;
+import java.util.LinkedHashMap;
+import java.util.List;
+
+/**
+ * Hides the implementation of the line groups list.
+ * If we implement multi-sequence Genbank loading then 
+ * this may need to change a bit later on.
+ * 
+ * @author kp11
+ *
+ */
+public class LineGroupCache
+{
+	@SuppressWarnings("unused")
+	private static final long serialVersionUID = 1L;
+	
+	public static final String FORWARD_REF_DELIM = "###";
+	
+	private LinkedHashMap<Long, LineGroup> headers = new LinkedHashMap<Long, LineGroup>();
+	
+	private LinkedHashMap<Long, LineGroup> misc = new LinkedHashMap<Long, LineGroup>();
+	
+	/**
+	 * The Sequence line group.
+	 */
+	private LineGroup sequence;
+	
+	/**
+	 * The FeatureTable line group.
+	 */
+	private FeatureTable featureTable;
+	
+	/**
+	 * Determine which internal data structure is associated with the given group.
+	 * @param group LineGroup
+	 * @return LinkedHashMap
+	 */
+	protected LinkedHashMap<Long, LineGroup> findRelevantList(LineGroup group)
+	{
+		if (group instanceof FeatureHeader)
+		{
+			return headers;
+		}
+		
+		return misc;
+	}
+	
+	/**
+	 * Return the feature table line group.
+	 * 
+	 * @return FeatureTable
+	 */
+	public FeatureTable getFeatureTable()
+	{
+		return featureTable;
+	}
+	
+	/**
+	 * Return the sequence line group.
+	 * 
+	 * @return LineGroup
+	 */
+	public LineGroup getSequence()
+	{
+		return sequence;
+	}
+	
+	/**
+	 * Add a line group to the end of the line group list.
+	 * 
+	 * @param lineGroup LineGroup
+	 */
+	public synchronized void add(LineGroup lineGroup)
+	{
+		if (lineGroup instanceof FeatureTable)
+		{
+			featureTable = (FeatureTable)lineGroup;
+		}
+		else if (lineGroup instanceof Sequence)
+		{
+			sequence = lineGroup;
+		}
+		else if (lineGroup instanceof GFFMisc && FORWARD_REF_DELIM.equals(((GFFMisc) lineGroup).getLine()))
+		{
+			// Remove these forward ref delimiters as we don't need them.
+			return;
+		}
+		else
+		{
+			findRelevantList(lineGroup).put(lineGroup.getUniqueId(), lineGroup);
+		}
+	}
+
+	/**
+	 * Remove the given line group from this cache and return it.
+	 * @param lineGroup LineGroup
+	 * @return LineGroup
+	 */
+	public synchronized LineGroup remove(LineGroup lineGroup)
+	{	
+		LineGroup group = null;
+		
+		if (lineGroup instanceof FeatureTable)
+		{
+			group = featureTable;
+			featureTable = null;
+			
+		}
+		else if (lineGroup instanceof Sequence)
+		{
+			group = sequence;
+			sequence = null;
+		}
+		else 
+		{
+			group = findRelevantList(lineGroup).remove(lineGroup.getUniqueId());
+		}
+		
+		return group;
+	}
+    
+    /**
+     *  Create and return a new FeatureTable().  The new FeatureTable will be
+     *  added to line_groups in the appropriate place.
+     *  @return FeatureTable
+     **/
+    public synchronized FeatureTable createFeatureTable() 
+    {
+    	featureTable = new StreamFeatureTable();
+
+    	return featureTable;
+    }
+    
+    /**
+     *  Return the text of the "header" of this Entry or null if there is no
+     *  header.
+     *  @return String
+     **/
+    public synchronized String getHeadersAsText() 
+    {
+      int numLineGroups = headers.size() + misc.size();
+      
+      List<LineGroup> groups = new ArrayList<LineGroup>(numLineGroups);
+      
+      groups.addAll(misc.values());
+      groups.addAll(headers.values());
+      
+      final StringBuilder buffer = 
+    		  new StringBuilder( numLineGroups*80 );
+
+      for (LineGroup group : groups)
+      {
+    	  buffer.append(group.toString());
+      }
+      
+     if(buffer.length() > 0) 
+        return buffer.toString();
+      else 
+        return null;
+    }
+    
+    /**
+     * Return a list of all line groups in the cache.
+     * @return List
+     */
+    public synchronized List<LineGroup> getAllLineGroups()
+    {
+    	List<LineGroup> allGroups = new ArrayList<LineGroup>();
+    	allGroups.addAll(misc.values());
+    	allGroups.addAll(headers.values());
+    	//allGroups.addAll(features.values());
+    	
+    	if (featureTable != null)
+    		allGroups.add(featureTable);
+    	
+    	// Add sequence at the end
+    	if (sequence != null)
+    		allGroups.add(sequence);
+    	
+    	return allGroups;
+    }
+    
+    /**
+     * Clear all miscellaneous line groups.
+     */
+    public synchronized void clearMiscLineGroups()
+    {
+    	misc.clear();
+    }
+    
+    /**
+     * Clear the cache.
+     */
+    public synchronized void clear()
+    {
+    	headers.clear();
+    	misc.clear();
+    	sequence = null;
+    	featureTable = null;
+    }
+    
+    /**
+     * Write all line groups out to a given stream in the correct order.
+     * @param entry Entry
+     * @param writer Writer
+     * @throws IOException
+     */
+    public void writeToStream(final Entry entry, final Writer writer) throws IOException
+    {
+    	long num = 0;
+    	boolean hasSequence = false;
+    	boolean hasFeatureTable = false;
+    	
+    	for (java.util.Map.Entry<Long, LineGroup> miscGroup : misc.entrySet())
+    	{
+    		miscGroup.getValue().writeToStream(writer);
+    		++num;
+    	}
+    	
+    	for (java.util.Map.Entry<Long, LineGroup> headerGroup : headers.entrySet())
+        {
+        	headerGroup.getValue().writeToStream(writer);
+        	++num;
+        }
+    	
+    	if (featureTable != null)
+    	{
+    		featureTable.writeToStream(writer);
+    		hasFeatureTable = true;
+    	}
+    	
+    	if (sequence != null)
+    	{
+    		if(entry instanceof GFFDocumentEntry && sequence instanceof FastaStreamSequence)
+    		{
+    			LineGroup.writeStartOfGFFEntry(writer);
+    		}
+    		
+    		sequence.writeToStream(writer);
+    		hasSequence = true;
+    	}
+    	
+    	if(num == 0L && (hasFeatureTable ^ hasSequence)) 
+        {
+    		// Don't write out the "//" end of entry marker if we have only one
+    		// LineGroup (feature table or sequence)
+    		// - this makes life easier for external programs that need
+    		// to read the feature table or sequence
+    		return;
+        }
+    	
+    	if(num == 0L && (hasFeatureTable && hasSequence)) 
+        {
+    		// Don't write out the "//" end of entry marker if this is raw or
+    		// FASTA sequence
+    		if(sequence instanceof RawStreamSequence ||
+    			sequence instanceof FastaStreamSequence) 
+    			return;
+        }
+
+    	// Else write out the end delimiter
+        if(entry instanceof PublicDBDocumentEntry) 
+          LineGroup.writeEndOfEMBLEntry(writer);
+    	
+    }
+
+}
diff --git a/src/main/java/uk/ac/sanger/artemis/io/LineGroupIdGenerator.java b/src/main/java/uk/ac/sanger/artemis/io/LineGroupIdGenerator.java
new file mode 100644
index 000000000..9394366d9
--- /dev/null
+++ b/src/main/java/uk/ac/sanger/artemis/io/LineGroupIdGenerator.java
@@ -0,0 +1,28 @@
+package uk.ac.sanger.artemis.io;
+
+import java.util.concurrent.atomic.AtomicLong;
+
+/**
+ * An ID generator for creating line group 
+ * unique IDs. We could use a UUID for this but
+ * that's twice the size of a Long!
+ * <br/>
+ * Thread Safe.
+ * 
+ * @author kp11
+ *
+ */
+public class LineGroupIdGenerator
+{
+	/** A counter variable */
+	private static AtomicLong idCounter = new AtomicLong();
+
+	/**
+	 * Increment the counter and return the new value.
+	 * @return Long
+	 */
+	public static Long createID()
+	{
+	    return idCounter.getAndIncrement();
+	}
+}
diff --git a/src/main/java/uk/ac/sanger/artemis/io/SimpleDocumentEntry.java b/src/main/java/uk/ac/sanger/artemis/io/SimpleDocumentEntry.java
index b2f00a7ab..5ce773b26 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/SimpleDocumentEntry.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/SimpleDocumentEntry.java
@@ -4,7 +4,7 @@
  *
  * This file is part of Artemis
  *
- * Copyright(C) 2000  Genome Research Limited
+ * Copyright(C) 2019  Genome Research Limited
  *
  * This program is free software; you can redistribute it and/or
  * modify it under the terms of the GNU General Public License
@@ -20,7 +20,6 @@
  * along with this program; if not, write to the Free Software
  * Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA  02111-1307, USA.
  *
- * $Header: //tmp/pathsoft/artemis/uk/ac/sanger/artemis/io/SimpleDocumentEntry.java,v 1.30 2009-09-03 13:33:18 tjc Exp $
  */
 
 package uk.ac.sanger.artemis.io;
@@ -28,20 +27,17 @@
 import uk.ac.sanger.artemis.util.*;
 
 import java.io.*;
-import java.util.Collections;
 import java.util.Date;
-import java.util.HashMap;
 import java.util.HashSet;
 import java.util.Set;
 import java.util.Vector;
 import java.util.Hashtable;
-import java.util.Map;
+import java.util.List;
 
 /**
  *  This class contains the methods common to all DocumentEntry objects.
  *
  *  @author Kim Rutherford <kmr@sanger.ac.uk>
- *  @version $Id: SimpleDocumentEntry.java,v 1.30 2009-09-03 13:33:18 tjc Exp $
  **/
 
 abstract public class SimpleDocumentEntry
@@ -71,7 +67,7 @@ abstract public class SimpleDocumentEntry
    *  This contains all the lines(stored as LineGroup objects) from the entry
    *  stream that was passed to the constructor.
    **/
-  protected Vector<LineGroup> line_groups = new Vector<LineGroup>();
+  protected LineGroupCache line_groups = new LineGroupCache();
                                                                                                                
   /**
    *  The DocumentEntryAutosaveThread that is started when the first call is
@@ -183,7 +179,6 @@ public SimpleDocumentEntry(final EntryInformation entry_information,
         final int[] header_positions =
                       fasta_sequence.getFastaHeaderPositions();
 
-        //final FeatureTable feature_table = 
         getFeatureTable();
 
         for(int i = 0 ; i < header_strings.length ; ++i) 
@@ -354,21 +349,7 @@ else if(docFeature != null)
    **/
   public String getHeaderText() 
   {
-    final StringBuffer buffer = new StringBuffer();
-
-    for(int i = 0 ; i < line_groups.size() ; ++i) 
-    {
-      final LineGroup current_line_group = line_groups.elementAt(i);
-
-      if(!(current_line_group instanceof FeatureTable) &&
-         !(current_line_group instanceof Sequence)) 
-        buffer.append(current_line_group.toString());
-    }
-
-    if(buffer.length() > 0) 
-      return buffer.toString();
-    else 
-      return null;
+	return line_groups.getHeadersAsText();
   }
 
   /**
@@ -416,19 +397,13 @@ public boolean setHeaderText(final String new_header)
 
     // now remove all the EmblMisc and GenbankMisc LineGroup objects from
     // this Entry
-    for(int i = line_groups.size() - 1 ; i >= 0  ; --i) 
-    {
-      final LineGroup current_line_group = line_groups.elementAt(i);
-
-      if(current_line_group instanceof MiscLineGroup) 
-        line_groups.removeElementAt(i);
-    }
+    line_groups.clearMiscLineGroups();
 
     if(new_header != null) 
     {
       // then add the new LineGroup objects
       for(int i = 0 ; i < new_line_groups.size() ; ++i) 
-        line_groups.insertElementAt(new_line_groups.elementAt(i), i);
+    	  line_groups.add(new_line_groups.elementAt(i));
     }
 
     setDirtyFlag();
@@ -448,39 +423,7 @@ public void writeToStream(final Writer writer)
 
     try
     {
-      for(int i = 0 ; i < line_groups.size() ; ++i) 
-      {
-        final LineGroup current_line_group = line_groups.elementAt(i);
-        if(this instanceof GFFDocumentEntry && 
-           current_line_group instanceof FastaStreamSequence)
-          LineGroup.writeStartOfGFFEntry(writer);
-        current_line_group.writeToStream(writer);
-      }
-
-      if(line_groups.size() == 1) 
-      {
-        // don't write out the "//" end of entry marker if we have only one
-        // LineGroup - this makes life easier for external programs that need
-        // to read the feature table or sequence
-        return;
-      }
-      else
-      {
-        if(line_groups.size() == 2) 
-        {
-          final LineGroup second_line_group = line_groups.elementAt(1);
-
-          // don't write out the "//" end of entry marker if this is raw or
-          // FASTA sequence
-          if(second_line_group instanceof RawStreamSequence ||
-             second_line_group instanceof FastaStreamSequence) 
-            return;
-        }
-      }
-
-      if(this instanceof PublicDBDocumentEntry) 
-        LineGroup.writeEndOfEMBLEntry(writer);
-
+      line_groups.writeToStream(this, writer);
     } 
     finally
     {
@@ -493,7 +436,7 @@ public void writeToStream(final Writer writer)
    **/
   public int getFeatureCount() 
   {
-    final FeatureTable feature_table = findFeatureTable();
+    final FeatureTable feature_table = line_groups.getFeatureTable();
 
     if(feature_table == null) 
       return 0;
@@ -695,7 +638,7 @@ public Feature forcedAdd(final Feature feature)
    **/
   protected boolean removeInternal(Feature feature) 
   {
-    final FeatureTable feature_table = findFeatureTable();
+    final FeatureTable feature_table = line_groups.getFeatureTable();
 
     if(feature_table == null) 
       return false;
@@ -747,7 +690,7 @@ public boolean remove(Feature feature)
    **/
   public Feature getFeatureAtIndex(int i) 
   {
-    final FeatureTable feature_table = findFeatureTable();
+    final FeatureTable feature_table = line_groups.getFeatureTable();
 
     if(feature_table == null) 
       return null;
@@ -761,7 +704,7 @@ public Feature getFeatureAtIndex(int i)
    **/
   public int indexOf(final Feature feature) 
   {
-    final FeatureTable feature_table = findFeatureTable();
+    final FeatureTable feature_table = line_groups.getFeatureTable();
 
     if(feature_table == null) 
       return -1;
@@ -774,7 +717,7 @@ public int indexOf(final Feature feature)
    **/
   public boolean contains(final Feature feature) 
   {
-    final FeatureTable feature_table = findFeatureTable();
+    final FeatureTable feature_table = line_groups.getFeatureTable();
 
     if(feature_table == null) 
       return false;
@@ -827,7 +770,7 @@ else if(this instanceof GFFDocumentEntry)
    **/
   public FeatureVector getFeaturesInRange(Range range) 
   {
-    final FeatureTable feature_table = findFeatureTable();
+    final FeatureTable feature_table = line_groups.getFeatureTable();
 
     if(feature_table == null) 
       return new FeatureVector();
@@ -843,7 +786,7 @@ public FeatureVector getFeaturesInRange(Range range)
    **/
   public FeatureVector getAllFeatures() 
   {
-    final FeatureTable feature_table = findFeatureTable();
+    final FeatureTable feature_table = line_groups.getFeatureTable();
 
     if(feature_table == null) 
       return new FeatureVector();
@@ -859,7 +802,7 @@ public FeatureVector getAllFeatures()
    **/
   public FeatureEnumeration features() 
   {
-    final FeatureTable feature_table = findFeatureTable();
+    final FeatureTable feature_table = line_groups.getFeatureTable();
 
     if(feature_table == null)
     {
@@ -885,82 +828,23 @@ public Feature nextFeature() {
    **/
   public Sequence getSequence() 
   {
-    for(int i = 0 ; i < line_groups.size() ; ++i) 
-    {
-      final LineGroup current_line_group = line_groups.elementAt(i);
-
-      if(current_line_group instanceof Sequence)
-        return(Sequence) current_line_group;
-    }
-
-    return null;
+    return (Sequence)line_groups.getSequence();
   }
 
   /**
    *  Add a new LineGroup object to this Entry.
    *  @param new_line_group A new LineGroup to add.
    **/
-  private void addLineGroup(final LineGroup new_line_group) 
+  protected void addLineGroup(final LineGroup new_line_group) 
   {
-    if(new_line_group instanceof FeatureHeader) 
+    if(new_line_group instanceof GFFMisc)
     {
-      // insert FH lines before the Sequence and Features(if any)
-      for(int i = 0 ; i < line_groups.size() ; ++i) 
-      {
-        final LineGroup this_line_group = line_groups.elementAt(i);
-
-        if(this_line_group instanceof Feature ||
-            this_line_group instanceof FeatureTable ||
-            this_line_group instanceof Sequence) 
-        {
-          line_groups.insertElementAt(new_line_group, i);
-          return;
-        }
-      }
-    } 
-    else if(new_line_group instanceof Feature)
-    {
-      // Features before the Sequence and FeatureTable(if any)
-      for(int i = 0 ; i < line_groups.size() ; ++i) 
-      {
-        final LineGroup this_line_group = line_groups.elementAt(i);
-
-        if(this_line_group instanceof FeatureTable ||
-            this_line_group instanceof Sequence) 
-        {
-          line_groups.insertElementAt(new_line_group, i);
-          return;
-        }
-      }
-    } 
-    else if(!(new_line_group instanceof Sequence) &&
-            !(new_line_group instanceof FeatureTable)) 
-    {
-      if(new_line_group instanceof GFFMisc)
-      {
-        String line = ((GFFMisc)new_line_group).toString();
-        if(line.indexOf("FASTA") > -1)  // ignore
-          return;
-      }   
-
-      // insert before features and sequence
-      for(int i = 0 ; i < line_groups.size() ; ++i) 
-      {
-        final LineGroup this_line_group = line_groups.elementAt(i);
-
-        if(this_line_group instanceof Feature ||
-           this_line_group instanceof FeatureTable ||
-           this_line_group instanceof FeatureHeader ||
-           this_line_group instanceof Sequence)
-        {
-          line_groups.insertElementAt(new_line_group, i);
-          return;
-        }
-      }
+      String line = ((GFFMisc)new_line_group).getLine();
+      if(line.indexOf("FASTA") > -1)  // ignore
+        return;
     }
 
-    // otherwise add at end
-    line_groups.addElement(new_line_group);
+    line_groups.add(new_line_group);
   }
 
   /**
@@ -999,47 +883,6 @@ public EntryInformation getEntryInformation()
     return entry_information;
   }
 
-  /**
-   *  Create and return a new FeatureTable().  The new FeatureTable will be
-   *  added to line_groups in the appropriate place.
-   **/
-  private FeatureTable createFeatureTable() 
-  {
-    final FeatureTable new_feature_table;
-
-    new_feature_table = new StreamFeatureTable();
-
-    // put feature table just before SEQUENCE LineGroup
-    if(line_groups.size() > 0 &&           
-        line_groups.lastElement() instanceof Sequence) 
-      line_groups.insertElementAt(new_feature_table,
-                                   line_groups.size() - 1);
-    else  // no SEQUENCE so add the feature table at the end
-      line_groups.insertElementAt(new_feature_table, line_groups.size());
-
-    return new_feature_table;
-  }
-
-  /**
-   *  Return the FeatureTable of this DocumentEntry or null if there isn't one
-   *  yet.
-   **/
-  private FeatureTable findFeatureTable() 
-  {
-    final int line_groups_size = line_groups.size();
-
-    for(int i = 0; i < line_groups_size; ++i) 
-    {
-      final LineGroup current_line_group = line_groups.elementAt(i);
-
-      if(current_line_group instanceof FeatureTable) 
-        return (FeatureTable)current_line_group;
-    }
-
-    return null;
-  }
-
-
   /**
    *  Return the FeatureTable object from this entry(which will be created if
    *  this object does not already contain one).  This method has package
@@ -1048,12 +891,12 @@ private FeatureTable findFeatureTable()
    *  @return a FeatureTable object for this Entry.  the returned object is
    *    not a copy - changes to it will change the Entry object itself
    **/
-  private FeatureTable getFeatureTable() 
+  protected FeatureTable getFeatureTable() 
   {
-    final FeatureTable found_feature_table = findFeatureTable();
+    final FeatureTable found_feature_table = line_groups.getFeatureTable();
 
     if(found_feature_table == null) 
-      return createFeatureTable();
+      return line_groups.createFeatureTable();
     else
       return found_feature_table;
   }
@@ -1061,19 +904,13 @@ private FeatureTable getFeatureTable()
   /**
    *  Remove the given LineGroup from this SimpleDocumentEntry.
    **/
-  private void removeLineGroup(final LineGroup line_group) 
+  protected void removeLineGroup(final LineGroup line_group) 
   {
-    for(int i = 0 ; i < line_groups.size() ; ++i) 
-    {
-      final LineGroup current_line_group = line_groups.elementAt(i);
-
-      if(current_line_group == line_group) 
-      {
-        line_groups.removeElementAt(i);
-        setDirtyFlag();
-        return;
-      }
-    }
+	LineGroup group = line_groups.remove(line_group);
+	if (group != null)
+	{
+	  setDirtyFlag();	
+	}
   }
 
   /**
@@ -1224,7 +1061,7 @@ public void setSequence(final StreamSequence sequence)
   /**
    *  Add the elements of fake_fasta_features to the feature table.
    **/
-  private void addFakeFeatures() 
+  protected void addFakeFeatures() 
   {
     final FeatureTable feature_table = getFeatureTable();
 
@@ -1235,7 +1072,7 @@ private void addFakeFeatures()
   /**
    *  Remove the elements of fake_fasta_features from the feature table.
    **/
-  private void removeFakeFeatures() 
+  protected void removeFakeFeatures() 
   {
     final FeatureTable feature_table = getFeatureTable();
 
@@ -1262,8 +1099,6 @@ private void fireEvent(final ReadEvent event)
 
   public void dispose()
   {
-    for(int i=0; i<line_groups.size(); i++)
-      line_groups.removeElementAt(i);
-    line_groups = null;
+    line_groups.clear();
   }
 }
diff --git a/src/test/java/uk/ac/sanger/artemis/io/LineGroupCacheTest.java b/src/test/java/uk/ac/sanger/artemis/io/LineGroupCacheTest.java
new file mode 100644
index 000000000..26f6b580c
--- /dev/null
+++ b/src/test/java/uk/ac/sanger/artemis/io/LineGroupCacheTest.java
@@ -0,0 +1,461 @@
+/* 
+ * This file is part of Artemis
+ *
+ * Copyright (C) 2019  Genome Research Limited
+ *
+ * This program is free software; you can redistribute it and/or
+ * modify it under the terms of the GNU General Public License
+ * as published by the Free Software Foundation; either version 2
+ * of the License, or (at your option) any later version.
+ *
+ * This program is distributed in the hope that it will be useful,
+ * but WITHOUT ANY WARRANTY; without even the implied warranty of
+ * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+ * GNU General Public License for more details.
+ *
+ * You should have received a copy of the GNU General Public License
+ * along with this program; if not, write to the Free Software
+ * Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA  02111-1307, USA.
+ *
+ */
+package uk.ac.sanger.artemis.io;
+
+import static org.junit.Assert.*;
+
+import java.io.StringReader;
+import java.io.StringWriter;
+import java.util.LinkedHashMap;
+import java.util.List;
+
+import org.junit.After;
+import org.junit.Before;
+import org.junit.Test;
+import org.mockito.Mock;
+import org.mockito.MockitoAnnotations;
+
+import uk.ac.sanger.artemis.util.LinePushBackReader;
+
+/**
+ * JUnit test for the LineGroupCache class.
+ * 
+ * @author kp11
+ *
+ */
+public class LineGroupCacheTest
+{
+
+	private static final String EMBL_HDR = "FH   Key             Location/Qualifiers\n";
+	
+	private static final String EMBL_MISC1 = "ID   1    standard; DNA; HTG; 200111453 BP.\n";
+	private static final String EMBL_MISC2 = "AC   chromosome:WSB_EiJ_v1:1:1:200111453:1\n";
+	
+	// Hacked sequence
+	private static final String EMBL_SEQUENCE = 
+			"SQ   Sequence  10 BP;   11 A;   12 C;   13 G;   14 T;   15 other;\n" + 
+			"     actgaaacag ttcctgcctg gctttgctga aaggggtaat aaaatcttct gtgtaaaggc        60\n";
+	
+	private static final String EMBL_FEATURE = 
+			"FT   CDS             1..10\n" + 
+			"FT                   /note=test\n";
+	
+	private static final int NUM_EMBL_HDRS_FOR_TESTS = 4;
+	
+	private static final int NUM_EMBL_MISC_FEATURES_FOR_TESTS = 2;
+	
+	private LineGroupCache groups = new LineGroupCache();
+	
+	// Test line groups
+	private LineGroup hdr1;
+	private LineGroup hdr2;
+	private LineGroup hdr3;
+	private LineGroup hdr4;
+	private EmblMisc misc1;
+	private EmblMisc misc2;
+	private EmblStreamFeature feature;
+	private FeatureTable ft;
+	private StreamSequence sequence;
+	
+	@Mock 
+	private PublicDBDocumentEntry entry;
+	
+	
+	/**
+	 * Add some test data to our line group cache.
+	 * 
+	 * @throws Exception
+	 */
+	@Before
+	public void setUp() throws Exception 
+	{
+		MockitoAnnotations.initMocks(this);
+		
+		// Add some headers
+		hdr1 = new FeatureHeader(new LinePushBackReader(new StringReader(EMBL_HDR)));
+		groups.add(hdr1);
+		hdr2 = new FeatureHeader(new LinePushBackReader(new StringReader(EMBL_HDR)));
+		groups.add(hdr2);
+		hdr3 = new FeatureHeader(new LinePushBackReader(new StringReader(EMBL_HDR)));
+		groups.add(hdr3);
+		hdr4 = new FeatureHeader(new LinePushBackReader(new StringReader(EMBL_HDR)));
+		groups.add(hdr4);
+		
+		// Add some misc lien groups
+		misc1 = new EmblMisc(new LinePushBackReader(new StringReader(EMBL_MISC1)));
+		groups.add(misc1);
+		misc2 = new EmblMisc(new LinePushBackReader(new StringReader(EMBL_MISC2)));
+		groups.add(misc2);
+		
+		
+		// Create a feature table and add a feature
+		QualifierVector qualifiers = new QualifierVector();
+		ft = new StreamFeatureTable();
+		qualifiers.add(new Qualifier("note", "test"));
+		feature = new EmblStreamFeature(new Key("CDS"), new Location("1..10"), qualifiers);
+		ft.add(feature);
+		groups.add(ft);
+		
+		// Add a sequence
+		sequence = new EmblStreamSequence(new LinePushBackReader(new StringReader(EMBL_SEQUENCE)));
+		groups.add(sequence);
+	}
+	
+	/**
+	 * Clear up at end of each test.
+	 */
+	@After
+	public void tearDown()
+	{
+		groups = null;
+		hdr1 = hdr2 = hdr3 = hdr4 = null;
+		ft = null;
+		sequence = null;
+		
+	}
+	
+	/**
+	 * Test the findRelevantList() method.
+	 * Also implicitly uses the add() method.
+	 * @throws Exception
+	 */
+	@Test
+	public void testFindRelevantList() throws Exception
+	{
+		// When
+		
+		LinkedHashMap<Long, LineGroup> result = groups.findRelevantList(hdr1);
+		
+		// Then
+		
+		assertNotNull( hdr1.getUniqueId() );
+		assertTrue( hdr1.getUniqueId() >= 0 );
+		assertNotNull( result );
+		assertEquals( NUM_EMBL_HDRS_FOR_TESTS, result.size() );
+		assertEquals( hdr1, result.get(hdr1.getUniqueId()) );
+		assertNotNull( hdr2.getUniqueId() );
+		assertTrue( hdr2.getUniqueId() >= 0 );
+		assertEquals( hdr2, result.get(hdr2.getUniqueId()) );
+		
+		// When
+		
+		result = groups.findRelevantList(misc1);
+		
+		// Then
+		
+		assertNotNull( misc1.getUniqueId() );
+		assertTrue( misc1.getUniqueId() >= 0 );
+		assertNotNull( result );
+		assertEquals( NUM_EMBL_MISC_FEATURES_FOR_TESTS, result.size() );
+		
+	}
+	
+	/**
+	 * Test the getFeatureTable() method.
+	 * Also implicitly uses the add() method.
+	 */
+	@Test
+	public void testGetFeatureTable()
+	{
+		// When
+		
+		FeatureTable result = groups.getFeatureTable();
+		
+		// Then
+		
+		assertNotNull( result );
+		assertTrue( result instanceof FeatureTable );
+		assertEquals( ft, (FeatureTable)result );
+	}
+	
+	/**
+	 * Test the getSequence() method.
+	 * Also implicitly uses the add() method.
+	 * @throws Exception
+	 */
+	@Test
+	public void testGetSequence() throws Exception
+	{
+		// When
+		
+		LineGroup result = groups.getSequence();
+		
+		// Then
+		
+		assertNotNull( result );
+		assertTrue( result instanceof StreamSequence );
+		assertEquals( sequence, (StreamSequence)result );
+	}
+	
+	/**
+	 * Additional tests for the add() method.
+	 * @throws Exception
+	 */
+	@Test
+	public void testAdd() throws Exception
+	{
+		// When 
+		
+		groups.clearMiscLineGroups();
+		// This should not get added
+		groups.add(new GFFMisc(new LinePushBackReader(new StringReader(LineGroupCache.FORWARD_REF_DELIM))));
+		
+		List<LineGroup> lineGrpList = groups.getAllLineGroups();
+		
+		// Then
+		
+		// Misc list should be empty
+		assertEquals( NUM_EMBL_HDRS_FOR_TESTS + 2, lineGrpList.size() ); // +3 for feature table and sequence
+	}
+	
+	/**
+	 * Test the remove() method.
+	 * @throws Exception
+	 */
+	@Test
+	public void testRemove() throws Exception
+	{	
+		// When
+		
+		LineGroup removedHdr1 = groups.remove(hdr1);
+		
+		// Then
+		
+		assertNotNull( removedHdr1 );
+		assertEquals( hdr1, removedHdr1 );
+		assertEquals( NUM_EMBL_HDRS_FOR_TESTS-1, groups.findRelevantList(hdr2).size() );
+		
+		// When
+		
+		LineGroup removedHdr2 = groups.remove(hdr2);
+		
+		// Then
+		
+		assertNotNull( removedHdr2 );
+		assertEquals( hdr2, removedHdr2 );
+		assertEquals( NUM_EMBL_HDRS_FOR_TESTS-2, groups.findRelevantList(hdr2).size() );
+				
+		// When
+		
+		LineGroup removedMisc1 = groups.remove(misc1);
+		
+		// Then
+		
+		assertNotNull( removedMisc1 );
+		assertEquals( misc1, removedMisc1 );
+		assertEquals( NUM_EMBL_MISC_FEATURES_FOR_TESTS-1, groups.findRelevantList(misc1).size() );
+				
+		// When
+		
+		LineGroup removedFT = groups.remove(ft);
+		
+		// Then
+		
+		assertNotNull( removedFT );
+		assertEquals( ft, removedFT );
+		assertNull( groups.getFeatureTable() );
+		
+		// When
+		
+		LineGroup removedSeq = groups.remove(sequence);
+		
+		// Then
+		
+		assertNotNull( removedSeq );
+		assertEquals( sequence, removedSeq );
+		assertNull( groups.getSequence() );
+	}
+	
+	/**
+	 * Test the getHeadersAsText() method.
+	 */
+	@Test
+	public void testGetHeadersAsText()
+	{
+		// When
+		
+		String result = groups.getHeadersAsText();
+		
+		// Then
+		
+		assertEquals(EMBL_MISC1 + EMBL_MISC2 + EMBL_HDR + EMBL_HDR + EMBL_HDR + EMBL_HDR, result);
+		
+		// When
+		
+		groups.clear();
+		result = groups.getHeadersAsText();
+		
+		// Then
+		
+		assertNull(result);
+	}
+	
+	/**
+	 * Test the getAllLineGroups() method.
+	 */
+	@Test
+	public void testGetAllLineGroups()
+	{
+		
+		// When
+		
+		List<LineGroup> lineGrpList = groups.getAllLineGroups();
+		
+		// Then
+		
+		assertEquals( NUM_EMBL_MISC_FEATURES_FOR_TESTS + NUM_EMBL_HDRS_FOR_TESTS + 2, lineGrpList.size() ); // +2 for feature table and sequence
+		
+		// When
+		
+		groups.clear();
+		lineGrpList = groups.getAllLineGroups();
+				
+		// Then
+				
+		assertEquals( 0, lineGrpList.size() );
+	}
+	
+	/**
+	 * Test the clear() method.
+	 */
+	@Test
+	public void testClear()
+	{
+		// When
+		
+		groups.clear();
+		List<LineGroup> lineGrpList = groups.getAllLineGroups();
+		
+		// Then
+		
+		assertNotNull( lineGrpList );
+		assertEquals( 0, lineGrpList.size() );	
+		
+		// Check multiple calls
+		groups.clear();
+		groups.clear();
+	}
+	
+	/**
+	 * Test the clearMiscLineGroups() method.
+	 */
+	@Test
+	public void testClearMiscLineGroups()
+	{
+		// When
+		
+		groups.clearMiscLineGroups();
+		List<LineGroup> lineGrpList = groups.getAllLineGroups();
+		
+		// Then
+		
+		assertEquals( NUM_EMBL_HDRS_FOR_TESTS + 2, lineGrpList.size() ); // +2 for feature table and sequence
+		
+		// Check multiple calls
+		groups.clearMiscLineGroups();
+		groups.clearMiscLineGroups();
+	}
+	
+	
+	/**
+	 * Test the writeToStream() method.
+	 */
+	@Test
+	public void testWriteToStream() throws Exception
+	{
+		// =============================================
+		// Test with headers, feature table and sequence
+		// =============================================
+		
+		// Given 
+		
+		StringWriter writer = new StringWriter();
+		
+		// When
+		
+		groups.writeToStream(entry, writer);
+		String result = writer.toString();
+		
+		// Then
+		
+		assertNotNull(result);
+		
+		assertEquals(
+				EMBL_MISC1 + 
+				EMBL_MISC2 + 
+				EMBL_HDR + 
+				EMBL_HDR + 
+				EMBL_HDR + 
+				EMBL_HDR + 
+				EMBL_FEATURE +
+				EMBL_SEQUENCE +
+				"//\n", 
+				result);
+		
+		// =============================================
+		// Test with sequence only
+		// =============================================
+		
+		// Given 
+		
+		writer = new StringWriter();
+		
+		// When
+		
+		groups.clear();
+		groups.add(sequence);
+		groups.writeToStream(entry, writer);
+		result = writer.toString();
+		
+		// Then
+		
+		assertNotNull(result);
+		
+		assertEquals(
+				EMBL_SEQUENCE, 
+				result);
+		
+		// =============================================
+		// Test with feature table only
+		// =============================================
+		
+		// Given 
+		
+		writer = new StringWriter();
+		ft.add(feature);
+		
+		// When
+		
+		groups.clear();
+		groups.add(ft);
+		groups.writeToStream(entry, writer);
+		result = writer.toString();
+
+		// Then
+		
+		assertNotNull(result);
+		
+		assertEquals(
+				EMBL_FEATURE, 
+				result);
+	}
+	
+}
diff --git a/src/test/java/uk/ac/sanger/artemis/io/LineGroupIdGeneratorTest.java b/src/test/java/uk/ac/sanger/artemis/io/LineGroupIdGeneratorTest.java
new file mode 100644
index 000000000..ab33d8351
--- /dev/null
+++ b/src/test/java/uk/ac/sanger/artemis/io/LineGroupIdGeneratorTest.java
@@ -0,0 +1,48 @@
+/* 
+ * This file is part of Artemis
+ *
+ * Copyright (C) 2019  Genome Research Limited
+ *
+ * This program is free software; you can redistribute it and/or
+ * modify it under the terms of the GNU General Public License
+ * as published by the Free Software Foundation; either version 2
+ * of the License, or (at your option) any later version.
+ *
+ * This program is distributed in the hope that it will be useful,
+ * but WITHOUT ANY WARRANTY; without even the implied warranty of
+ * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+ * GNU General Public License for more details.
+ *
+ * You should have received a copy of the GNU General Public License
+ * along with this program; if not, write to the Free Software
+ * Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA  02111-1307, USA.
+ *
+ */
+package uk.ac.sanger.artemis.io;
+
+import static org.junit.Assert.assertEquals;
+
+import org.junit.Test;
+
+/**
+ * Unit test for the LineGroupIdGenerator class.
+ * 
+ * @author kp11
+ *
+ */
+public class LineGroupIdGeneratorTest
+{
+
+	/**
+	 * Test the createID() method.
+	 */
+	@Test
+	public void testCreateID()
+	{
+		for (long i = 0L; i < 1000L; i++)
+		{
+			assertEquals(i, LineGroupIdGenerator.createID().longValue());
+		}
+	}
+	
+}
diff --git a/src/test/java/uk/ac/sanger/artemis/io/SimpleDocumentEntryTest.java b/src/test/java/uk/ac/sanger/artemis/io/SimpleDocumentEntryTest.java
new file mode 100644
index 000000000..4d4e9b519
--- /dev/null
+++ b/src/test/java/uk/ac/sanger/artemis/io/SimpleDocumentEntryTest.java
@@ -0,0 +1,1208 @@
+/* 
+ * This file is part of Artemis
+ *
+ * Copyright (C) 2019  Genome Research Limited
+ *
+ * This program is free software; you can redistribute it and/or
+ * modify it under the terms of the GNU General Public License
+ * as published by the Free Software Foundation; either version 2
+ * of the License, or (at your option) any later version.
+ *
+ * This program is distributed in the hope that it will be useful,
+ * but WITHOUT ANY WARRANTY; without even the implied warranty of
+ * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+ * GNU General Public License for more details.
+ *
+ * You should have received a copy of the GNU General Public License
+ * along with this program; if not, write to the Free Software
+ * Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA  02111-1307, USA.
+ *
+ */
+package uk.ac.sanger.artemis.io;
+
+import static org.junit.Assert.*;
+import static org.mockito.Mockito.when;
+
+import java.io.BufferedReader;
+import java.io.File;
+import java.io.FileInputStream;
+import java.io.FileOutputStream;
+import java.io.FileWriter;
+import java.io.IOException;
+import java.io.InputStreamReader;
+import java.io.OutputStream;
+import java.net.URL;
+import java.util.zip.GZIPInputStream;
+
+import org.apache.commons.lang3.StringUtils;
+import org.junit.Before;
+import org.junit.Test;
+import org.mockito.Mock;
+import org.mockito.MockitoAnnotations;
+
+import uk.ac.sanger.artemis.EntryGroup;
+import uk.ac.sanger.artemis.SimpleEntryGroup;
+import uk.ac.sanger.artemis.sequence.Bases;
+import uk.ac.sanger.artemis.util.FileDocument;
+
+
+/**
+ * JUnit test suite for the SimpleDocumentEntry class
+ * and underlying functionality (more of an integration test really!).
+ * 
+ * This test module carries out multiple tests in each test method
+ * - this is done for efficiency reasons to avoid having to 
+ *   repeatedly load test files.
+ * 
+ * @author kp11
+ *
+ */
+public class SimpleDocumentEntryTest
+{
+
+	// GFF
+	
+	// Indexed
+	private final String INDEXED_GFF_FILE = "/data/gff/indexed-gff.gff.gz";
+	private final String INDEXED_GFF_FASTA_FILE = "/data/gff/indexed-gff.fa";
+	private final int NUM_INDEXED_GFF_FEATURES = 2238;
+	
+	// Non-indexed
+	private final String GFF_FILE = "/data/gff/notindexed.gff.gz";
+	
+	private final String GFF_OUTPUT_FILE = "out.gff3";
+	private final int NUM_GFF_FEATURES = 1584;
+	private final int GFF_SEQ_LEN = 1587953;
+	private final String GFF_FEATURE_ID_KEY = "ID";
+	
+	private final String GFF_SEQ_START = "tgaaccctaaaacctaaaccctaaaccctaaaccctgaaccctaaaccctgaaccctaaaccctaaaccctgaac";
+	private final String GFF_SEQ_END = "tagggttcagggtttaggtgtcagggttca";
+			
+	private final String GFF_HDRS = 
+			"##gff-version 3\n" + 
+			"##sequence-region Pf3D7_01_v3 1 640851\n" + 
+			"##sequence-region Pf3D7_02_v3 1 947102\n";
+	
+	// GTF
+	
+	private final String GTF_FILE = "/data/gtf/clostridium_cellulosi.gtf.gz";
+	
+	private final String GTF_OUTPUT_FILE = "out.gtf";
+	private final String GTF_FASTA_FILE = "/data/fasta/clostridium_cellulosi.fa.gz";
+	private final int NUM_GTF_FEATURES = 12315;
+	private final int GTF_SEQ_LEN = 2229578;
+	
+	private final String GTF_SEQ_START = "atgcagtccttttcggaagtatggt";
+	private final String GTF_SEQ_END = "aaacaaattaaatgttcggaggtttaagagc";
+	
+	private final String GTF_HDRS = 
+			"#!genome-build DG5\n" + 
+			"#!genome-version DG5\n" + 
+			"#!genome-date 2014-08\n" + 
+			"#!genome-build-accession GCA_000953215.1\n" + 
+			"#!genebuild-last-updated 2014-08\n";
+	
+	// EMBL
+	
+	private final String EMBL_FILE = "/data/embl/MAL1.embl.gz";
+	private final String EMBL_OUTPUT_FILE = "out.embl";
+	private final int NUM_EMBL_FEATURES = 248;
+	private final int EMBL_SEQ_LEN = 643292;
+	private final String EMBL_FEATURE_ID_KEY = "locus_tag";
+	
+	private final String EMBL_SEQ_START = "ctaaacctaaacctaaaccctgaaccctaaaccctaaaccctgaaccctaaaccctgaaccctgaaccctaaac";
+	private final String EMBL_SEQ_END = "ttagggttcagggtttagggtttagggtttagggaatagggt";
+		
+	private final String EMBL_HDRS = 
+			"ID   Pf3D7_01.embl; SV ; ; ; ; ; 643292 BP.\n" + 
+			"FH   Key             Location/Qualifiers\n" + 
+			"FH\n";
+	
+	// Genbank
+	
+	private final String GBK_FILE = "/data/genbank/SeaOtter_chrMT.gbk.gz";
+	private final String GBK_OUTPUT_FILE = "out.gbk";
+	private final int NUM_GBK_FEATURES = 53;
+	private final int GBK_SEQ_LEN = 16431;
+	
+	private final String GBK_SEQ_START = "gttaatgtagcttataaataaagcaaggcactgaaaatgcctagaagagtcataagactc" + 
+			"catagacataaaggtttggtcctagccttcctattaattattaacagaattacacatgca";
+	private final String GBK_SEQ_END = "acttatactggtgccacgcatgttaatctcacttactaatccattaaaacttcctattcaaaatgaagc" +
+			"tatctatagatgtgaattcccacctctatcacccccggactt";
+		
+	private final String GBK_HDRS = 
+			"LOCUS       NC_009692              16431 bp    DNA     circular MAM 15-APR-2009\n" + 
+			"DEFINITION  Enhydra lutris mitochondrion, complete genome.\n" + 
+			"ACCESSION   NC_009692\n" + 
+			"VERSION     NC_009692.1\n" + 
+			"DBLINK      Project: 20145\n" + 
+			"            BioProject: PRJNA20145\n" + 
+			"KEYWORDS    RefSeq.\n" + 
+			"SOURCE      mitochondrion Enhydra lutris (sea otter)\n" + 
+			"  ORGANISM  Enhydra lutris\n" + 
+			"            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;\n" + 
+			"            Mammalia; Eutheria; Laurasiatheria; Carnivora; Caniformia;\n" + 
+			"            Mustelidae; Lutrinae; Enhydra.\n" + 
+			"REFERENCE   1  (bases 1 to 16431)\n" + 
+			"  AUTHORS   Yonezawa,T., Nikaido,M., Kohno,N., Fukumoto,Y., Okada,N. and\n" + 
+			"            Hasegawa,M.\n" + 
+			"  TITLE     Molecular phylogenetic study on the origin and evolution of\n" + 
+			"            Mustelidae\n" + 
+			"  JOURNAL   Gene 396 (1), 1-12 (2007)\n" + 
+			"   PUBMED   17449200\n" + 
+			"REFERENCE   2  (bases 1 to 16431)\n" + 
+			"  CONSRTM   NCBI Genome Project\n" + 
+			"  TITLE     Direct Submission\n" + 
+			"  JOURNAL   Submitted (24-JUL-2007) National Center for Biotechnology\n" + 
+			"            Information, NIH, Bethesda, MD 20894, USA\n" + 
+			"REFERENCE   3  (bases 1 to 16431)\n" + 
+			"  AUTHORS   Yonezawa,T., Nikaido,M., Kohno,N., Fukumoto,Y., Okada,N. and\n" + 
+			"            Hasegawa,M.\n" + 
+			"  TITLE     Direct Submission\n" + 
+			"  JOURNAL   Submitted (22-JAN-2007) The Graduate University for Advanced\n" + 
+			"            Studies (SOKENDAI), The Department of Biosystems Science; Shonan\n" + 
+			"            Village, Hayama, Kanagawa 240-0193, Japan\n" + 
+			"COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The\n" + 
+			"            reference sequence was derived from AB291077.\n" + 
+			"            COMPLETENESS: full length.\n" + 
+			"FEATURES             Location/Qualifiers\n";
+	
+	// Fasta
+	
+	private final String FASTA_FILE = "/data/fasta/test.fa.gz";
+	private final String FASTA_OUTPUT_FILE = "out.fa";
+	private final int FASTA_SEQ_LEN = 240;
+	private final String FASTA_TEXT = 
+			"atgacaaggcttccattactaaaacgacctcgcagaaaccgaaaaagtgcagccgttcga" + 
+			"tctataattcaagaaacccaactctgttctagtgacttgatctggcccatctttcttaaa" + 
+			"tctataattcaagaaacccaactctgttctagtgacttgatctggcccatctttcttaaa" + 
+			"ttgacaaggcttccattactaaaacgacctcgcagaaaccgaaaaagtgcagccgttcga";
+	
+	// Blast
+	private final String BLAST_FILE = "/data/blast/blast_test_results.blast";
+	private final String BLAST_FASTA_FILE = "/data/blast/blast_test.fa";
+	private final String BLAST_OUTPUT_FILE = "out.blast";
+	
+	private final int NUM_BLAST_FEATURES = 46;
+	
+	public static final boolean LOG = false;
+	
+	@Mock
+	private FileDocument saveToDoc;
+	
+	@Before
+	public void setUp() throws Exception {
+
+		MockitoAnnotations.initMocks(this);
+	}
+	
+	
+	// =========================================================
+	// Utility methods
+	// =========================================================
+	
+	protected File getFile(String filePath)
+	{
+		URL filePathUrl = SimpleDocumentEntryTest.class.getResource(filePath);
+		File file = new File(filePathUrl.getFile());
+		
+		return file;
+	}
+	
+	// Basic file comparison
+	protected boolean doBasicFileComparison(File file1, File file2, boolean ignoreCase) throws IOException
+	{
+		boolean result = true;
+		
+		final boolean isFile1Zipped = file1.getName().endsWith(".gz");
+		final boolean isFile2Zipped = file2.getName().endsWith(".gz");
+		
+		try (
+			final BufferedReader fileStream1 = new BufferedReader( 
+					new InputStreamReader(
+							( isFile1Zipped ?  new GZIPInputStream(new FileInputStream(file1)) : new FileInputStream(file1) ) 
+					)
+			);
+			final BufferedReader fileStream2 = new BufferedReader( 
+					new InputStreamReader(
+							( isFile2Zipped ?  new GZIPInputStream(new FileInputStream(file2)) : new FileInputStream(file2) ) 
+					)
+			);
+		)
+		{
+			boolean finished = false;
+			while (!finished)
+			{
+				String line1 = fileStream1.readLine();
+				if (line1 != null)
+					line1 = line1.replaceAll("\\r\\n|\\r|\\n", "");
+				
+				String line2 = fileStream2.readLine();
+				if (line2 != null)
+					line2 = line2.replaceAll("\\r\\n|\\r|\\n", "");
+				
+				result = ignoreCase ? StringUtils.equalsIgnoreCase(line1, line2) : StringUtils.equals(line1, line2);
+				if (!result)
+					return result;
+				
+				if (line1 == null && line2 == null) 
+				{
+					finished = true;
+				}
+			}
+		}
+		
+		return result;
+	}
+	
+	protected void printFeatures(String title, Entry entry) throws InvalidRelationException
+	{
+		final FeatureEnumeration features = entry.features();
+		int i = 0;
+		
+		System.out.println(title + ": ");
+				
+		while (features.hasMoreFeatures())
+		{
+			Feature ft = features.nextFeature();
+			
+			if (ft.getQualifierByName(GFF_FEATURE_ID_KEY) != null)
+			{
+				log("printFeatures",
+						"" + i + ": " + ft.getKey().getKeyString() + " " + 
+						ft.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) + " " +
+						ft.getFirstBase() + " " +
+						ft.getLastBase());
+			}
+			else
+			{
+				log("printFeatures", "" + i + ": " + ft.getKey().getKeyString());
+			}
+			
+			++i;
+		}
+	}
+	
+	protected void printFeatures(String title, uk.ac.sanger.artemis.Entry entry) throws InvalidRelationException
+	{
+		final int numFeatures = entry.getFeatureCount();
+		
+		System.out.println(title + ": ");
+				
+		for (int i = 0; i < numFeatures; i++)
+		{
+			uk.ac.sanger.artemis.Feature ft = entry.getFeature(i);
+			
+			if (ft.getQualifierByName(GFF_FEATURE_ID_KEY) != null)
+			{
+				log("printFeatures",
+						"" + i + ": " + ft.getKey().getKeyString() + " " + 
+						ft.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) + " " +
+						ft.getFirstBase() + " " +
+						ft.getLastBase());
+			}
+			else
+			{
+				log("printFeatures", "" + i + ": " + ft.getKey().getKeyString());
+			}
+		}
+	}
+	
+	/**
+	 * Load a data file.
+	 * @param file String
+	 * @return Entry
+	 */
+	protected Entry loadFile(String file)
+	{
+		long startTime = System.currentTimeMillis();
+		final Entry entry = Utils.getEntry(file);
+		long endTime = System.currentTimeMillis();
+		
+		log("loadFile", "File load time (" + file + "): " + (endTime - startTime) + " milliseconds");
+		
+		return entry;
+	}
+	
+	/**
+	 * Output some useful info for testing purposes.
+	 * @param method String
+	 * @param message String
+	 */
+	public void log(String method, String message)
+	{
+		if (LOG)
+		{
+			System.out.println(method + ": " + message);
+		}
+	}
+	
+	// =========================================================
+	// Tests
+	// =========================================================
+	
+	/**
+	 * Exercise Embl file loading/saving.
+	 * 
+	 * @throws Exception
+	 */
+	@Test
+	public void testEmblFile() throws Exception
+	{
+		// Given
+		
+		final File savefile = File.createTempFile(EMBL_OUTPUT_FILE, "");
+		final OutputStream outStream = new FileOutputStream(savefile);
+		
+		log("testEmblFile", "Writing to file: " + savefile.getAbsolutePath());
+
+		// When
+		
+		when( saveToDoc.getFile() ).thenReturn (savefile);
+		when( saveToDoc.getWriter() ).thenReturn (new FileWriter(savefile));
+		when( saveToDoc.getOutputStream() ).thenReturn (outStream);
+		
+		final Entry entry = loadFile(EMBL_FILE);
+		
+		assertTrue(entry instanceof EmblDocumentEntry); 
+		
+		((EmblDocumentEntry)entry).save(saveToDoc);
+		
+		
+		// Then
+		
+		assertEquals( NUM_EMBL_FEATURES, entry.getFeatureCount() );
+		assertEquals( EMBL_HDRS, entry.getHeaderText() );
+		
+		Sequence sequence = entry.getSequence();
+		String seqString = String.valueOf( sequence.getCharSubSequence(1, sequence.length()) );
+		
+		assertTrue(sequence instanceof EmblStreamSequence);
+		assertEquals( EMBL_SEQ_LEN, seqString.length() );
+		assertEquals( 261151, sequence.getACount() );
+		assertEquals( 65283, sequence.getCCount() );
+		assertEquals( 66886, sequence.getGCount() );
+		assertEquals( 249972, sequence.getTCount() );
+		assertTrue(seqString.indexOf(EMBL_SEQ_START) == 0);
+		assertTrue(seqString.lastIndexOf(EMBL_SEQ_END) == (seqString.length()-EMBL_SEQ_END.length()));
+
+		//printFeatures("testEmblFile", entry);
+		
+		// Check the feature table
+		assertNotNull(((SimpleDocumentEntry)entry).getFeatureTable());
+		assertEquals(NUM_EMBL_FEATURES, ((SimpleDocumentEntry)entry).getFeatureTable().getFeatureCount());
+		
+		int idx = 0;
+		
+		// Repeat region
+		
+		Feature nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "repeat_region", nextFeature.getKey().getKeyString() );
+		assertEquals( "Pfalciparum_REP_20", nextFeature.getQualifierByName(EMBL_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 1, nextFeature.getFirstBase() );
+		assertEquals( 583, nextFeature.getLastBase() );
+				
+		idx = 14;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "ncRNA", nextFeature.getKey().getKeyString() );
+		assertEquals( "RNAzID:26", nextFeature.getQualifierByName(EMBL_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 40820, nextFeature.getFirstBase() );
+		assertEquals( 41021, nextFeature.getLastBase() );
+			
+		idx = 18;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "CDS", nextFeature.getKey().getKeyString() );
+		assertEquals( "PFA0020w", nextFeature.getQualifierByName(EMBL_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 50586, nextFeature.getFirstBase() );
+		assertEquals( 51859, nextFeature.getLastBase() );
+		
+		nextFeature = entry.getFeatureAtIndex(++idx);
+		assertEquals( "CDS_motif", nextFeature.getKey().getKeyString() );
+		assertEquals( "cdsMotif50846-50860", nextFeature.getQualifierByName(EMBL_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 50847, nextFeature.getFirstBase() );
+		assertEquals( 50860, nextFeature.getLastBase() );
+		
+		idx = 75;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "5'UTR", nextFeature.getKey().getKeyString() );
+		assertEquals( "PFA0180w:mRNA", nextFeature.getQualifierByName(EMBL_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 161286, nextFeature.getFirstBase() );
+		assertEquals( 161365, nextFeature.getLastBase() );
+		
+		idx = 83;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "3'UTR", nextFeature.getKey().getKeyString() );
+		assertEquals( "PFA0205w:mRNA", nextFeature.getQualifierByName(EMBL_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 181026, nextFeature.getFirstBase() );
+		assertEquals( 181049, nextFeature.getLastBase() );
+		
+		idx = 88;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "CDS", nextFeature.getKey().getKeyString() );
+		assertEquals( "PFA0225w", nextFeature.getQualifierByName(EMBL_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 202774, nextFeature.getFirstBase() );
+		assertEquals( 204381, nextFeature.getLastBase() );
+		
+		// Test indexOf and contains
+		assertEquals(idx, entry.indexOf(nextFeature));
+		assertTrue(entry.contains(nextFeature));
+		
+		idx = 177;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "rRNA", nextFeature.getKey().getKeyString() );
+		assertEquals( "MAL1_28s", nextFeature.getQualifierByName(EMBL_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 478428, nextFeature.getFirstBase() );
+		assertEquals( 482531, nextFeature.getLastBase() );
+		
+		// The very last feature
+		
+		idx = 247;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "repeat_region", nextFeature.getKey().getKeyString() );
+		assertEquals( "Pfalciparum_REP_45", nextFeature.getQualifierByName(EMBL_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 641897, nextFeature.getFirstBase() );
+		assertEquals( 642097, nextFeature.getLastBase() );
+		
+		// Test indexOf and contains
+		assertEquals(idx, entry.indexOf(nextFeature));
+		assertTrue(entry.contains(nextFeature));
+		
+	}
+	
+	/**
+	 * Exercise GenBank file loading/saving.
+	 * 
+	 * @throws Exception
+	 */
+	@Test
+	public void testGenbankFile() throws Exception
+	{
+		// Given
+		
+		final File savefile = File.createTempFile(GBK_OUTPUT_FILE, "");
+		final OutputStream outStream = new FileOutputStream(savefile);
+		
+		log("testGenbankFile", "Writing to file: " + savefile.getAbsolutePath());
+		
+		// When
+		
+		when( saveToDoc.getFile() ).thenReturn (savefile);
+		when( saveToDoc.getWriter() ).thenReturn (new FileWriter(savefile));
+		when( saveToDoc.getOutputStream() ).thenReturn (outStream);
+		
+		final Entry entry = loadFile(GBK_FILE);
+		
+		assertTrue(entry instanceof GenbankDocumentEntry);
+		
+		((GenbankDocumentEntry)entry).save(saveToDoc);
+		
+		
+		// Then
+		
+		assertEquals( NUM_GBK_FEATURES, entry.getFeatureCount() );
+		assertEquals( GBK_HDRS, entry.getHeaderText() );
+		
+		Sequence sequence = entry.getSequence();
+		String seqString = String.valueOf( sequence.getCharSubSequence(1, sequence.length()) );
+		
+		assertTrue(sequence instanceof GenbankStreamSequence);
+		assertEquals( GBK_SEQ_LEN, seqString.length() );
+		assertEquals( 5340, sequence.getACount() );
+		assertEquals( 4418, sequence.getCCount() );
+		assertEquals( 2337, sequence.getGCount() );
+		assertEquals( 4336, sequence.getTCount() );
+		assertTrue(seqString.indexOf(GBK_SEQ_START) == 0);
+		assertTrue(seqString.lastIndexOf(GBK_SEQ_END) == (seqString.length()-GBK_SEQ_END.length()));
+
+		//printFeatures("testGenbankFile", entry);
+		
+		// Check the feature table
+		assertNotNull(((SimpleDocumentEntry)entry).getFeatureTable());
+		assertEquals(NUM_GBK_FEATURES, ((SimpleDocumentEntry)entry).getFeatureTable().getFeatureCount());
+		
+		int idx = 0;
+		
+		// Repeat region
+		
+		Feature nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "source", nextFeature.getKey().getKeyString() );
+		assertEquals( "Enhydra lutris", nextFeature.getQualifierByName("organism").getValues().elementAt(0) );
+		assertEquals( 1, nextFeature.getFirstBase() );
+		assertEquals( 16431, nextFeature.getLastBase() );
+				
+		idx = 14;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "tRNA", nextFeature.getKey().getKeyString() );
+		assertEquals( "tRNA-Ala", nextFeature.getQualifierByName("product").getValues().elementAt(0) );
+		assertEquals( 5032, nextFeature.getFirstBase() );
+		assertEquals( 5100, nextFeature.getLastBase() );
+		
+		idx = 16;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "rep_origin", nextFeature.getKey().getKeyString() );
+		assertNull( nextFeature.getQualifierByName("product") );
+		assertEquals( 5175, nextFeature.getFirstBase() );
+		assertEquals( 5210, nextFeature.getLastBase() );
+			
+		idx = 19;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "gene", nextFeature.getKey().getKeyString() );
+		assertEquals( "COX1", nextFeature.getQualifierByName("gene").getValues().elementAt(0) );
+		assertEquals( 5344, nextFeature.getFirstBase() );
+		assertEquals( 6888, nextFeature.getLastBase() );
+		
+		nextFeature = entry.getFeatureAtIndex(++idx);
+		assertEquals( "CDS", nextFeature.getKey().getKeyString() );
+		assertEquals( "COX1", nextFeature.getQualifierByName("gene").getValues().elementAt(0) );
+		assertEquals( 5344, nextFeature.getFirstBase() );
+		assertEquals( 6888, nextFeature.getLastBase() );
+		
+		// Test indexOf and contains
+		assertEquals(idx, entry.indexOf(nextFeature));
+		assertTrue(entry.contains(nextFeature));
+		
+		idx = 49;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "CDS", nextFeature.getKey().getKeyString() );
+		assertEquals( "CYTB", nextFeature.getQualifierByName("gene").getValues().elementAt(0) );
+		assertEquals( "1", nextFeature.getQualifierByName("codon_start").getValues().elementAt(0) );
+		assertEquals( "2", nextFeature.getQualifierByName("transl_table").getValues().elementAt(0) );
+		assertEquals( "cytochrome b", nextFeature.getQualifierByName("product").getValues().elementAt(0) );
+		assertEquals( "YP_001382358.1", nextFeature.getQualifierByName("protein_id").getValues().elementAt(0) );
+		assertEquals( "GeneID:5333285", nextFeature.getQualifierByName("db_xref").getValues().elementAt(0) );
+		assertEquals( 14175, nextFeature.getFirstBase() );
+		assertEquals( 15314, nextFeature.getLastBase() );
+		
+		// The very last feature
+		
+		idx = 52;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "D-loop", nextFeature.getKey().getKeyString() );
+		assertNull( nextFeature.getQualifierByName("product") );
+		assertEquals( 15448, nextFeature.getFirstBase() );
+		assertEquals( 16431, nextFeature.getLastBase() );
+		
+		// Test indexOf and contains
+		assertEquals(idx, entry.indexOf(nextFeature));
+		assertTrue(entry.contains(nextFeature));
+		
+	}
+	
+	/**
+	 * Exercise non-indexed GFF file loading/saving.
+	 * 
+	 * @throws Exception
+	 */
+	@Test
+	public void testNonIndexedGFF() throws Exception
+	{
+		// Given
+		
+		final File savefile = File.createTempFile(GFF_OUTPUT_FILE, "");
+		final OutputStream outStream = new FileOutputStream(savefile);
+		
+		log("testNonIndexedGFF", "Writing to file: " + savefile.getAbsolutePath());
+		
+		// When
+		
+		when( saveToDoc.getFile() ).thenReturn (savefile);
+		when( saveToDoc.getWriter() ).thenReturn (new FileWriter(savefile));
+		when( saveToDoc.getOutputStream() ).thenReturn (outStream);
+		
+		final Entry entry = loadFile(GFF_FILE);
+		
+		assertTrue(entry instanceof GFFDocumentEntry);
+		
+		((GFFDocumentEntry)entry).save(saveToDoc);
+		
+		
+		// Then
+		
+		assertEquals( NUM_GFF_FEATURES, entry.getFeatureCount() );
+		assertEquals( GFF_HDRS, entry.getHeaderText() );
+		
+		Sequence sequence = entry.getSequence();
+		String seqString = String.valueOf( sequence.getCharSubSequence(1, sequence.length()) );
+		
+		assertTrue(sequence instanceof FastaStreamSequence);
+		assertEquals( GFF_SEQ_LEN, seqString.length() );
+		assertEquals( 639816, sequence.getACount() );
+		assertEquals( 159003, sequence.getCCount() );
+		assertEquals( 159425, sequence.getGCount() );
+		assertEquals( 629709, sequence.getTCount() );
+		
+		assertTrue(seqString.indexOf(GFF_SEQ_START) == 0);
+		assertTrue(seqString.lastIndexOf(GFF_SEQ_END) == (seqString.length()-GFF_SEQ_END.length()));
+
+		//printFeatures("testNonIndexedGFF", entry);
+		
+		// Check the feature table
+		assertNotNull(((SimpleDocumentEntry)entry).getFeatureTable());
+		assertEquals(NUM_GFF_FEATURES, ((SimpleDocumentEntry)entry).getFeatureTable().getFeatureCount());
+		
+		int idx = 0;
+		
+		// Repeat region
+		
+		Feature nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "repeat_region", nextFeature.getKey().getKeyString() );
+		assertEquals( "Pfalciparum_REP_20", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 1, nextFeature.getFirstBase() );
+		assertEquals( 360, nextFeature.getLastBase() );
+				
+		// Gene
+		
+		idx = 20;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "gene", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0200300", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 35927, nextFeature.getFirstBase() );
+		assertEquals( 37249, nextFeature.getLastBase() );
+				
+		nextFeature = entry.getFeatureAtIndex(++idx);
+		assertEquals( "CDS", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0200300.1:exon{1}", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 35927, nextFeature.getFirstBase() );
+		assertEquals( 37249, nextFeature.getLastBase() );
+		
+		nextFeature = entry.getFeatureAtIndex(++idx);
+		assertEquals( "polypeptide", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0200300.1:pep", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 35927, nextFeature.getFirstBase() );
+		assertEquals( 37249, nextFeature.getLastBase() );
+		
+		nextFeature = entry.getFeatureAtIndex(++idx);
+		assertEquals( "mRNA", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0200300.1", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 35927, nextFeature.getFirstBase() );
+		assertEquals( 37249, nextFeature.getLastBase() );
+		
+		// Next gene
+		
+		nextFeature = entry.getFeatureAtIndex(++idx);
+		assertEquals( "gene", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0200400", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 38287, nextFeature.getFirstBase() );
+		assertEquals( 39303, nextFeature.getLastBase() );
+		
+		nextFeature = entry.getFeatureAtIndex(++idx);
+		assertEquals( "CDS", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0200400.1:exon{2,1}", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 38287, nextFeature.getFirstBase() );
+		assertEquals( 39303, nextFeature.getLastBase() );
+		
+		nextFeature = entry.getFeatureAtIndex(++idx);
+		assertEquals( "mRNA", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0200400.1", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 38287, nextFeature.getFirstBase() );
+		assertEquals( 39303, nextFeature.getLastBase() );
+		
+		nextFeature = entry.getFeatureAtIndex(++idx);
+		assertEquals( "polypeptide", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0200400.1:pep", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 38287, nextFeature.getFirstBase() );
+		assertEquals( 39303, nextFeature.getLastBase() );
+		
+		// Test indexOf and contains
+		assertEquals(idx, entry.indexOf(nextFeature));
+		assertTrue(entry.contains(nextFeature));
+		
+		// Pseudogene
+		
+		idx = 1535;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "pseudogene", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0222300", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 883452, nextFeature.getFirstBase() );
+		assertEquals( 884312, nextFeature.getLastBase() );
+		
+		nextFeature = entry.getFeatureAtIndex(++idx);
+		assertEquals( "polypeptide", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0222300.1:pep", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 883452, nextFeature.getFirstBase() );
+		assertEquals( 884312, nextFeature.getLastBase() );
+		
+		nextFeature = entry.getFeatureAtIndex(++idx);
+		assertEquals( "pseudogenic_transcript", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0222300.1", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 883452, nextFeature.getFirstBase() );
+		assertEquals( 884312, nextFeature.getLastBase() );
+		
+		nextFeature = entry.getFeatureAtIndex(++idx);
+		assertEquals( "pseudogenic_exon", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0222300.1:exon{1}", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 883452, nextFeature.getFirstBase() );
+		assertEquals( 884312, nextFeature.getLastBase() );
+		
+		// The very last feature
+		
+		idx = 1583;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "repeat_region", nextFeature.getKey().getKeyString() );
+		assertEquals( "Pf3D7_02__new:946548..947102", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 946548, nextFeature.getFirstBase() );
+		assertEquals( 947102, nextFeature.getLastBase() );
+		
+		// Test indexOf and contains
+		assertEquals(idx, entry.indexOf(nextFeature));
+		assertTrue(entry.contains(nextFeature));
+		
+	}
+	
+	/**
+	 * Exercise indexed GFF file loading/saving.
+	 * 
+	 * @throws Exception
+	 */
+	@Test
+	public void testIndexedGFF() throws Exception
+	{
+		// Given
+		
+		final File savefile = File.createTempFile(GFF_OUTPUT_FILE, "");
+		final OutputStream outStream = new FileOutputStream(savefile);
+		
+		log("testIndexedGFF", "Writing to file: " + savefile.getAbsolutePath());
+		
+		// We don't want GUI pop-ups displayed during unit tests...
+		DocumentEntryFactory.setDisplayIndexingQuestionForGffs(false);
+		
+		
+		// When
+		
+		when( saveToDoc.getFile() ).thenReturn (savefile);
+		when( saveToDoc.getWriter() ).thenReturn (new FileWriter(savefile));
+		when( saveToDoc.getOutputStream() ).thenReturn (outStream);
+		
+		final Entry entry = loadFile(INDEXED_GFF_FILE);
+		final Entry fastaEntry = loadFile(INDEXED_GFF_FASTA_FILE);
+		
+		final Bases bases = new Bases(fastaEntry.getSequence());
+		final EntryGroup group = new SimpleEntryGroup(bases);
+		final uk.ac.sanger.artemis.Entry artFastaEntry = new uk.ac.sanger.artemis.Entry(bases, fastaEntry);
+		final uk.ac.sanger.artemis.Entry artGffEntry = new uk.ac.sanger.artemis.Entry(bases, entry);
+		group.add(artFastaEntry);
+		group.add(artGffEntry);
+		
+		((IndexedGFFDocumentEntry)entry).save(saveToDoc);
+	
+		
+	    // Then
+		
+		assertTrue(entry instanceof IndexedGFFDocumentEntry);
+		assertEquals( NUM_INDEXED_GFF_FEATURES, artGffEntry.getFeatureCount() );
+		assertNull( artGffEntry.getHeaderText() ); // Method not implemented for GFF
+
+		
+		String seqString = artGffEntry.getBases().toString();
+		
+		assertEquals( GFF_SEQ_LEN, seqString.length() );
+		assertTrue(seqString.indexOf(GFF_SEQ_START) == 0);
+		assertTrue(seqString.lastIndexOf(GFF_SEQ_END) == (seqString.length()-GFF_SEQ_END.length()));
+
+		//printFeatures("testIndexedGFF", artGffEntry);
+		
+		int idx = 0;
+		
+		// Repeat region
+		
+		uk.ac.sanger.artemis.Feature nextFeature = artGffEntry.getFeature(idx);
+		assertEquals( "repeat_region", nextFeature.getKey().getKeyString() );
+		assertEquals( "Pfalciparum_REP_20", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 1, nextFeature.getFirstBase() );
+		assertEquals( 360, nextFeature.getLastBase() );
+				
+		// Gene
+		
+		idx = 12;
+		nextFeature = artGffEntry.getFeature(idx);
+		assertEquals( "gene", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0100200", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 1547747, nextFeature.getFirstBase() );
+		assertEquals( 1548972, nextFeature.getLastBase() );
+		
+		nextFeature = artGffEntry.getFeature(++idx);
+		assertEquals( "mRNA", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0100200.1", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 1547747, nextFeature.getFirstBase() );
+		assertEquals( 1548972, nextFeature.getLastBase() );
+		
+		nextFeature = artGffEntry.getFeature(++idx);
+		assertEquals( "polypeptide", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0100200.1:pep", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 1547747, nextFeature.getFirstBase() );
+		assertEquals( 1548972, nextFeature.getLastBase() );
+		
+		nextFeature = artGffEntry.getFeature(++idx);
+		assertEquals( "CDS", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0100200.1:exon:2", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 1547747, nextFeature.getFirstBase() );
+		assertEquals( 1547800, nextFeature.getLastBase() );
+		
+		idx = 22;
+		nextFeature = artGffEntry.getFeature(idx);
+		assertEquals( "gene", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0100400", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 50363, nextFeature.getFirstBase() );
+		assertEquals( 51636, nextFeature.getLastBase() );
+		
+		nextFeature = artGffEntry.getFeature(++idx);
+		assertEquals( "mRNA", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0100400.1", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 50363, nextFeature.getFirstBase() );
+		assertEquals( 51636, nextFeature.getLastBase() );
+		
+		nextFeature = artGffEntry.getFeature(++idx);
+		assertEquals( "polypeptide", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0100400.1:pep", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 50363, nextFeature.getFirstBase() );
+		assertEquals( 51636, nextFeature.getLastBase() );
+		
+		nextFeature = artGffEntry.getFeature(++idx);
+		assertEquals( "CDS", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0100400.1:exon:2", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 50572, nextFeature.getFirstBase() );
+		assertEquals( 51636, nextFeature.getLastBase() );
+		
+		// Pseudogene
+		
+		idx = 2069;
+		nextFeature = artGffEntry.getFeature(idx);
+		assertEquals( "pseudogene", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0220400", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 1461280, nextFeature.getFirstBase() );
+		assertEquals( 1463392, nextFeature.getLastBase() );
+		
+		nextFeature = artGffEntry.getFeature(++idx);
+		assertEquals( "pseudogenic_exon", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0220400.1:exon:1", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 1461280, nextFeature.getFirstBase() );
+		assertEquals( 1461318, nextFeature.getLastBase() );
+		
+		nextFeature = artGffEntry.getFeature(++idx);
+		assertEquals( "pseudogenic_transcript", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0220400.1", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 1461280, nextFeature.getFirstBase() );
+		assertEquals( 1463392, nextFeature.getLastBase() );
+		
+		nextFeature = artGffEntry.getFeature(++idx);
+		assertEquals( "pseudogenic_exon", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0220400.1:exon:2", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 1461420, nextFeature.getFirstBase() );
+		assertEquals( 1461977, nextFeature.getLastBase() );
+		
+		nextFeature = artGffEntry.getFeature(++idx);
+		assertEquals( "pseudogenic_exon", nextFeature.getKey().getKeyString() );
+		assertEquals( "PF3D7_0220400.1:exon:3", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 1461980, nextFeature.getFirstBase() );
+		assertEquals( 1463392, nextFeature.getLastBase() );
+		
+		// The very last feature
+		
+		idx = 2237;
+		nextFeature = artGffEntry.getFeature(idx);
+		assertEquals( "repeat_region", nextFeature.getKey().getKeyString() );
+		assertEquals( "Pf3D7_02__new:946548..947102", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
+		assertEquals( 1587399, nextFeature.getFirstBase() );
+		assertEquals( 1587953, nextFeature.getLastBase() );
+		
+		// Test indexOf and contains
+		Feature testFeature = entry.getFeatureAtIndex(10);
+		assertEquals(10, entry.indexOf(testFeature));
+		assertTrue(entry.contains(testFeature));
+		
+		testFeature = entry.getFeatureAtIndex(70);
+		assertEquals(70, entry.indexOf(testFeature));
+		assertTrue(entry.contains(testFeature));
+		
+	}
+	
+	/**
+	 * Exercise Fasta file loading/saving.
+	 * 
+	 * @throws Exception
+	 */
+	@Test
+	public void testNonIndexedFastaFile() throws Exception
+	{
+		// Given
+		
+		File savefile = File.createTempFile(FASTA_OUTPUT_FILE, "");
+		final OutputStream outStream = new FileOutputStream(savefile);
+		
+		log("testNonIndexedFastaFile", "Writing to file: " + savefile.getAbsolutePath());
+		
+		// We don't want GUI pop-ups displayed during unit tests...
+		DocumentEntryFactory.setDisplayIndexingQuestionForGffs(false);
+		
+		
+		// When
+		
+		when( saveToDoc.getFile() ).thenReturn (savefile);
+		when( saveToDoc.getWriter() ).thenReturn (new FileWriter(savefile));
+		when( saveToDoc.getOutputStream() ).thenReturn (outStream);
+		
+		final Entry entry = loadFile(FASTA_FILE);
+		
+		assertTrue(entry instanceof EmblDocumentEntry);
+			
+		((EmblDocumentEntry)entry).save(saveToDoc);
+			
+		//printFeatures("testNonIndexedFastaFile", entry);
+		
+		// Then
+		
+		final Sequence sequence = entry.getSequence();
+
+		assertTrue(sequence instanceof FastaStreamSequence);
+		assertEquals(FASTA_SEQ_LEN, sequence.length());
+		assertEquals(FASTA_TEXT, String.valueOf( sequence.getCharSubSequence(1, sequence.length()) ));
+		// Check no. of bases
+		assertEquals(38, sequence.getGCount());
+		assertEquals(77, sequence.getACount());
+		assertEquals(63, sequence.getTCount());
+		assertEquals(62, sequence.getCCount());
+		
+		// Check Fasta headers
+		String[] hdrs = ((FastaStreamSequence)sequence).getFastaHeaderStrings();
+		assertNotNull(hdrs);
+		assertEquals(2, hdrs.length);
+		assertEquals("AM884176.1", hdrs[0]);
+		assertEquals("AM884177.1", hdrs[1]);
+		
+		// Check the saved file is what we expect...
+		assertTrue(doBasicFileComparison(savefile, getFile(FASTA_FILE), true));
+		
+	}
+	
+	/**
+	 * Exercise GTF file loading/saving.
+	 * 
+	 * @throws Exception
+	 */
+	@Test
+	public void testGTF() throws Exception
+	{
+		// Given
+		
+		final File savefile = File.createTempFile(GTF_OUTPUT_FILE, "");
+		final OutputStream outStream = new FileOutputStream(savefile);
+		
+		log("testGTF", "Writing to file: " + savefile.getAbsolutePath());
+		
+		// When
+		
+		when( saveToDoc.getFile() ).thenReturn (savefile);
+		when( saveToDoc.getWriter() ).thenReturn (new FileWriter(savefile));
+		when( saveToDoc.getOutputStream() ).thenReturn (outStream);
+		
+		final Entry entry = loadFile(GTF_FILE);
+		final Entry fastaEntry = loadFile(GTF_FASTA_FILE);
+		
+		final Bases bases = new Bases(fastaEntry.getSequence());
+		final EntryGroup group = new SimpleEntryGroup(bases);
+		final uk.ac.sanger.artemis.Entry artFastaEntry = new uk.ac.sanger.artemis.Entry(bases, fastaEntry);
+		final uk.ac.sanger.artemis.Entry artGffEntry = new uk.ac.sanger.artemis.Entry(bases, entry);
+		group.add(artFastaEntry);
+		group.add(artGffEntry);
+		
+		((GFFDocumentEntry)entry).save(saveToDoc);
+		
+		
+		// Then
+		
+		assertEquals( NUM_GTF_FEATURES, entry.getFeatureCount() );
+		assertEquals( GTF_HDRS, entry.getHeaderText() );
+		
+		Sequence sequence = fastaEntry.getSequence();
+		String seqString = String.valueOf( sequence.getCharSubSequence(1, sequence.length()) );
+		
+		assertTrue(sequence instanceof FastaStreamSequence);
+		assertEquals( GTF_SEQ_LEN, seqString.length() );
+		assertEquals( 620990, sequence.getACount() );
+		assertEquals( 492667, sequence.getCCount() );
+		assertEquals( 491650, sequence.getGCount() );
+		assertEquals( 624271, sequence.getTCount() );
+		
+		assertTrue(seqString.indexOf(GTF_SEQ_START) == 0);
+		assertTrue(seqString.lastIndexOf(GTF_SEQ_END) == (seqString.length()-GTF_SEQ_END.length()));
+
+		// printFeatures("testGTF", entry);
+		
+		// Check the feature table
+		assertNotNull(((SimpleDocumentEntry)entry).getFeatureTable());
+		assertEquals(NUM_GTF_FEATURES, ((SimpleDocumentEntry)entry).getFeatureTable().getFeatureCount());
+		
+		int idx = 0;
+		
+		// Gene
+		
+		Feature nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "gene", nextFeature.getKey().getKeyString() );
+		assertEquals( "CCDG5_0001", nextFeature.getQualifierByName("gene_id").getValues().elementAt(0) );
+		assertEquals( "dnaA", nextFeature.getQualifierByName("gene_name").getValues().elementAt(0) );
+		assertEquals( 1, nextFeature.getFirstBase() );
+		assertEquals( 1332, nextFeature.getLastBase() );
+				
+		// Transcript
+		
+		idx = 14;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "transcript", nextFeature.getKey().getKeyString() );
+		assertEquals( "CCDG5_0003", nextFeature.getQualifierByName("gene_id").getValues().elementAt(0) );
+		assertEquals( "CDZ23154", nextFeature.getQualifierByName("transcript_id").getValues().elementAt(0) );
+		assertEquals( "ena", nextFeature.getQualifierByName("gene_source").getValues().elementAt(0) );
+		assertEquals( "protein_coding", nextFeature.getQualifierByName("gene_biotype").getValues().elementAt(0) );
+		assertNull( nextFeature.getQualifierByName("transcript_name") );
+		assertEquals( "ena", nextFeature.getQualifierByName("transcript_source").getValues().elementAt(0) );
+		assertEquals( "protein_coding", nextFeature.getQualifierByName("transcript_biotype").getValues().elementAt(0) );
+		assertEquals( 2740, nextFeature.getFirstBase() );
+		assertEquals( 2961, nextFeature.getLastBase() );
+		
+		// Exon
+		
+		idx = 64;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "exon", nextFeature.getKey().getKeyString() );
+		assertEquals( "CCDG5_0011", nextFeature.getQualifierByName("gene_id").getValues().elementAt(0) );
+		assertEquals( "CDZ23162", nextFeature.getQualifierByName("transcript_id").getValues().elementAt(0) );
+		assertEquals( "ena", nextFeature.getQualifierByName("gene_source").getValues().elementAt(0) );
+		assertEquals( "protein_coding", nextFeature.getQualifierByName("gene_biotype").getValues().elementAt(0) );
+		assertEquals( "ena", nextFeature.getQualifierByName("transcript_source").getValues().elementAt(0) );
+		assertEquals( "protein_coding", nextFeature.getQualifierByName("transcript_biotype").getValues().elementAt(0) );
+		assertEquals( "CDZ23162-1", nextFeature.getQualifierByName("exon_id").getValues().elementAt(0) );
+		assertEquals( 12722, nextFeature.getFirstBase() );
+		assertEquals( 13171, nextFeature.getLastBase() );
+		
+		// End features
+		
+		// CDS
+		
+		idx = 12313;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "CDS", nextFeature.getKey().getKeyString() );
+		assertEquals( "CCDG5_2088", nextFeature.getQualifierByName("gene_id").getValues().elementAt(0) );
+		assertEquals( "CDZ25168", nextFeature.getQualifierByName("transcript_id").getValues().elementAt(0) );
+		assertEquals( "ena", nextFeature.getQualifierByName("gene_source").getValues().elementAt(0) );
+		assertEquals( "protein_coding", nextFeature.getQualifierByName("gene_biotype").getValues().elementAt(0) );
+		assertEquals( "ena", nextFeature.getQualifierByName("transcript_source").getValues().elementAt(0) );
+		assertEquals( "protein_coding", nextFeature.getQualifierByName("transcript_biotype").getValues().elementAt(0) );
+		assertEquals( "1", nextFeature.getQualifierByName("exon_number").getValues().elementAt(0) );
+		assertEquals( 2226882, nextFeature.getFirstBase() );
+		assertEquals( 2228033, nextFeature.getLastBase() );
+		
+		// start_codon
+		
+		idx = 12314;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "start_codon", nextFeature.getKey().getKeyString() );
+		assertEquals( "CCDG5_2088", nextFeature.getQualifierByName("gene_id").getValues().elementAt(0) );
+		assertEquals( "CDZ25168", nextFeature.getQualifierByName("transcript_id").getValues().elementAt(0) );
+		assertEquals( "ena", nextFeature.getQualifierByName("gene_source").getValues().elementAt(0) );
+		assertEquals( "protein_coding", nextFeature.getQualifierByName("gene_biotype").getValues().elementAt(0) );
+		assertEquals( "ena", nextFeature.getQualifierByName("transcript_source").getValues().elementAt(0) );
+		assertEquals( "protein_coding", nextFeature.getQualifierByName("transcript_biotype").getValues().elementAt(0) );
+		assertEquals( "1", nextFeature.getQualifierByName("exon_number").getValues().elementAt(0) );
+		assertEquals( 2228031, nextFeature.getFirstBase() );
+		assertEquals( 2228033, nextFeature.getLastBase() );
+		
+		// stop_codon
+		
+		idx = 12310;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "stop_codon", nextFeature.getKey().getKeyString() );
+		assertEquals( "CCDG5_2088", nextFeature.getQualifierByName("gene_id").getValues().elementAt(0) );
+		assertEquals( "CDZ25168", nextFeature.getQualifierByName("transcript_id").getValues().elementAt(0) );
+		assertEquals( "ena", nextFeature.getQualifierByName("gene_source").getValues().elementAt(0) );
+		assertEquals( "protein_coding", nextFeature.getQualifierByName("gene_biotype").getValues().elementAt(0) );
+		assertEquals( "ena", nextFeature.getQualifierByName("transcript_source").getValues().elementAt(0) );
+		assertEquals( "protein_coding", nextFeature.getQualifierByName("transcript_biotype").getValues().elementAt(0) );
+		assertEquals( "1", nextFeature.getQualifierByName("exon_number").getValues().elementAt(0) );
+		assertEquals( 2226879, nextFeature.getFirstBase() );
+		assertEquals( 2226881, nextFeature.getLastBase() );
+		
+		// Test indexOf and contains
+		assertEquals(idx, entry.indexOf(nextFeature));
+		assertTrue(entry.contains(nextFeature));
+		
+	}
+	
+	/**
+	 * Exercise Blast file loading/saving.
+	 * 
+	 * @throws Exception
+	 */
+	@Test
+	public void testBlast() throws Exception
+	{
+		// Given
+		
+		final File savefile = File.createTempFile(BLAST_OUTPUT_FILE, "");
+		final OutputStream outStream = new FileOutputStream(savefile);
+		
+		log("testBlast", "Writing to file: " + savefile.getAbsolutePath());
+		
+		
+		// When
+		
+		when( saveToDoc.getFile() ).thenReturn (savefile);
+		when( saveToDoc.getWriter() ).thenReturn (new FileWriter(savefile));
+		when( saveToDoc.getOutputStream() ).thenReturn (outStream);
+		
+		final Entry entry = loadFile(BLAST_FILE);
+		final Entry fastaEntry = loadFile(BLAST_FASTA_FILE);
+		
+		final Bases bases = new Bases(fastaEntry.getSequence());
+		final EntryGroup group = new SimpleEntryGroup(bases);
+		final uk.ac.sanger.artemis.Entry artFastaEntry = new uk.ac.sanger.artemis.Entry(bases, fastaEntry);
+		final uk.ac.sanger.artemis.Entry artBlastEntry = new uk.ac.sanger.artemis.Entry(bases, entry);
+		group.add(artFastaEntry);
+		group.add(artBlastEntry);
+		
+		((BlastDocumentEntry)entry).save(saveToDoc);
+		
+		
+		// Then
+		
+		assertEquals( NUM_BLAST_FEATURES, entry.getFeatureCount() );
+		assertNull( entry.getHeaderText() );
+
+		//printFeatures("testBlast", entry);
+		
+		// Check the feature table
+		assertNotNull(((SimpleDocumentEntry)entry).getFeatureTable());
+		assertEquals(NUM_BLAST_FEATURES, ((SimpleDocumentEntry)entry).getFeatureTable().getFeatureCount());
+		
+		// Blast features
+	
+		// First
+		int idx = 0;
+		Feature nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "BLASTCDS", nextFeature.getKey().getKeyString() );
+		assertEquals( "15.9", nextFeature.getQualifierByName("blast_score").getValues().elementAt(0) );
+		assertEquals( "85.714", nextFeature.getQualifierByName("score").getValues().elementAt(0) );
+		assertEquals( "85.714", nextFeature.getQualifierByName("percent_id").getValues().elementAt(0) );
+		assertEquals( "C7D1KZ100B4HIU", nextFeature.getQualifierByName("query_id").getValues().elementAt(0) );
+		assertEquals( "3", nextFeature.getQualifierByName("subject_start").getValues().elementAt(0) );
+		assertEquals( "16", nextFeature.getQualifierByName("subject_end").getValues().elementAt(0) );
+		assertEquals( "200", nextFeature.getQualifierByName("subject_id").getValues().elementAt(0) );
+		assertEquals( "hit to 200 3..16  score: 15.9  percent id: 85.714  e-value: 3.8", nextFeature.getQualifierByName("note").getValues().elementAt(0) );
+		assertEquals( 3, nextFeature.getFirstBase() );
+		assertEquals( 16, nextFeature.getLastBase() );
+
+		// Last
+		idx = NUM_BLAST_FEATURES-1;
+		nextFeature = entry.getFeatureAtIndex(idx);
+		assertEquals( "BLASTCDS", nextFeature.getKey().getKeyString() );
+		assertEquals( "15.9", nextFeature.getQualifierByName("blast_score").getValues().elementAt(0) );
+		assertEquals( "100.000", nextFeature.getQualifierByName("score").getValues().elementAt(0) );
+		assertEquals( "100.000", nextFeature.getQualifierByName("percent_id").getValues().elementAt(0) );
+		assertEquals( "C7D1KZ100BYF90", nextFeature.getQualifierByName("query_id").getValues().elementAt(0) );
+		assertEquals( "181", nextFeature.getQualifierByName("subject_start").getValues().elementAt(0) );
+		assertEquals( "174", nextFeature.getQualifierByName("subject_end").getValues().elementAt(0) );
+		assertEquals( "200", nextFeature.getQualifierByName("subject_id").getValues().elementAt(0) );
+		assertEquals( "hit to 200 181..174  score: 15.9  percent id: 100.000  e-value: 3.8", nextFeature.getQualifierByName("note").getValues().elementAt(0) );
+		assertEquals( 174, nextFeature.getFirstBase() );
+		assertEquals( 181, nextFeature.getLastBase() );
+	}
+}

From 9faeb893c56387cceb2e8c1ee2e59028775043be Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Wed, 9 Oct 2019 10:19:22 +0100
Subject: [PATCH 11/25] Fixed removal of redundant delimiters

---
 src/main/java/uk/ac/sanger/artemis/io/LineGroupCache.java | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/src/main/java/uk/ac/sanger/artemis/io/LineGroupCache.java b/src/main/java/uk/ac/sanger/artemis/io/LineGroupCache.java
index 755cdceaf..5413a2691 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/LineGroupCache.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/LineGroupCache.java
@@ -85,9 +85,9 @@ else if (lineGroup instanceof Sequence)
 		{
 			sequence = lineGroup;
 		}
-		else if (lineGroup instanceof GFFMisc && FORWARD_REF_DELIM.equals(((GFFMisc) lineGroup).getLine()))
+		else if (lineGroup instanceof GFFMisc && ((GFFMisc) lineGroup).getLine().startsWith(FORWARD_REF_DELIM))
 		{
-			// Remove these forward ref delimiters as we don't need them.
+			// Remove these GFF delimiters as we don't need them.
 			return;
 		}
 		else

From 58edbefeebec97f897cedddec98873e6acbc835f Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Wed, 9 Oct 2019 12:27:30 +0100
Subject: [PATCH 12/25] Added additional file suffixes to the
 feature_file_suffixes and sequence_file_suffixes properties

---
 etc/options | 6 ++----
 1 file changed, 2 insertions(+), 4 deletions(-)

diff --git a/etc/options b/etc/options
index f102fcdb2..175dc09b7 100644
--- a/etc/options
+++ b/etc/options
@@ -2,8 +2,6 @@
 
 # (Note that comment lines start with a hash (#) symbol)
 
-# $Header: //tmp/pathsoft/artemis/etc/options,v 1.73 2009-08-06 16:15:19 tjc Exp $
-
 # This file should contain option settings that look like this:
 #
 # option_name = option_value
@@ -305,11 +303,11 @@ colour_of_GFF = 11
 colour_of_start_codon = 6
 
 # suffixes used on files that contain features - used in file requesters
-feature_file_suffixes = tab embl gbk genbank tab_embl gff feature feat \
+feature_file_suffixes = tab embl gbk genbank tab_embl gff gff3 feature feat \
    art artemis
 
 # suffixes used on files that contain sequence - used in file requesters
-sequence_file_suffixes = embl gbk genbank gff tab_embl seq dna \
+sequence_file_suffixes = embl gbk genbank gff gff3 tab_embl seq dna dat \
    art artemis fa fasta fas gb fna ffn
 
 # the default height for the base plot window

From 4e66db21ba92399b0200484de0ba9e40dddf33a3 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Wed, 9 Oct 2019 14:41:01 +0100
Subject: [PATCH 13/25] Minor change to stop validation aborting when a
 NullPointer is encountered

---
 .../java/uk/ac/sanger/artemis/io/ValidateFeature.java | 11 ++++++++++-
 1 file changed, 10 insertions(+), 1 deletion(-)

diff --git a/src/main/java/uk/ac/sanger/artemis/io/ValidateFeature.java b/src/main/java/uk/ac/sanger/artemis/io/ValidateFeature.java
index f2feb74dc..eb1779634 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/ValidateFeature.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/ValidateFeature.java
@@ -194,7 +194,16 @@ private LinkedHashMap<String, Level> featureValidate(final uk.ac.sanger.artemis.
     if(isGFF(f, entryGrp))
     {
       final GFFStreamFeature gffFeature = (GFFStreamFeature)f;
-      report.put("\n"+GeneUtils.getUniqueName(gffFeature)+" ("+fTxt+"):", Level.INFO);
+      
+      try 
+      {
+    	  report.put("\n"+GeneUtils.getUniqueName(gffFeature)+" ("+fTxt+"):", Level.INFO);
+      } 
+      catch (NullPointerException e)
+      {
+    	  // Fix to avoid the whole validation bombing out if an ID is not found
+    	  report.put("\n"+fTxt+"):", Level.INFO);
+      }
       
       if(isPartOfGene(gffFeature))
       {

From 9554332096535982906c3513610d8c1cee942262 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Thu, 10 Oct 2019 10:52:35 +0100
Subject: [PATCH 14/25] Changed max number of lines that can be displayed in
 the log window so that it doesn't hang

---
 etc/log4j.properties | 15 +++------------
 1 file changed, 3 insertions(+), 12 deletions(-)

diff --git a/etc/log4j.properties b/etc/log4j.properties
index 0ff647a61..83ce06506 100644
--- a/etc/log4j.properties
+++ b/etc/log4j.properties
@@ -57,7 +57,6 @@ log4j.logger.java.sql.ResultSet=INFO, R
 
 # j2ssh
 log4j.logger.com.sshtools.j2ssh=WARN, R
-# Pattern to output the caller's file name and line number.
 
 !-----------------------------------------------------------------------------!
 ! Configure appenders (log destinations/targets) and their options            !
@@ -66,13 +65,13 @@ log4j.logger.com.sshtools.j2ssh=WARN, R
 log4j.appender.stdout=org.apache.log4j.ConsoleAppender
 log4j.appender.stdout.layout=org.apache.log4j.PatternLayout
 log4j.appender.stdout.layout.ConversionPattern=%5p [%t] (%F:%L) - %m%n
-#log4j.appender.stdout.layout.ConversionPattern=%m%n
 log4j.appender.Default=uk.ac.sanger.artemis.components.LogViewer
 log4j.appender.R=uk.ac.sanger.artemis.components.LogViewer
-log4j.appender.R.MaxLogLines=2000
 log4j.appender.R.layout=org.apache.log4j.PatternLayout
-#log4j.appender.R.layout.ConversionPattern=%5p [%t] %c - %m%n
 log4j.appender.R.layout.ConversionPattern=%d{dd MMM HH:mm:ss} - %m%n
+# The next property determines the number of lines we see in the log viewer screen.
+# If it's too big then we will get display performance impacts
+log4j.appender.R.MaxLogLines=300
 
 !-------------------------RollingFileAppender OPTIONS-------------------------!
 log4j.appender.LOGFILE=org.apache.log4j.DailyRollingFileAppender
@@ -82,11 +81,3 @@ log4j.appender.LOGFILE.DatePattern='.'yyyy-MM-dd
 #log4j.appender.LOGFILE.Threshold=INFO
 log4j.appender.LOGFILE.layout=org.apache.log4j.PatternLayout
 log4j.appender.LOGFILE.layout.ConversionPattern=%d{dd MMM yyyy HH:mm:ss,SSS} %-4r [%t] %-5p %c %x - %m%n
-
-
-#log4j.appender.srv=org.apache.log4j.net.SocketAppender
-#log4j.appender.srv.layout=org.apache.log4j.PatternLayout
-#log4j.appender.srv.RemoteHost=localhost
-#log4j.appender.srv.Port=10105
-#log4j.appender.srv.LocationInfo=false
-#log4j.appender.srv.layout.ConversionPattern=%d{dd MMM yyyy HH:mm:ss,SSS} %X{username} %X{host} %-4r [%t] %-5p %c %x - %m%n

From 0d36c6ab05c6c3df72a4f2f9b4b0c5056c0a4027 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Fri, 11 Oct 2019 15:31:23 +0100
Subject: [PATCH 15/25] Refactored LineGroupCache

---
 .../uk/ac/sanger/artemis/io/LineGroup.java    | 12 ----
 .../ac/sanger/artemis/io/LineGroupCache.java  | 63 ++++++++++--------
 .../artemis/io/LineGroupIdGenerator.java      | 28 --------
 .../artemis/io/SimpleDocumentEntry.java       | 22 +++++--
 .../sanger/artemis/io/LineGroupCacheTest.java | 64 ++++++-------------
 .../artemis/io/LineGroupIdGeneratorTest.java  | 48 --------------
 .../artemis/io/SimpleDocumentEntryTest.java   | 52 +++++++++++++++
 7 files changed, 121 insertions(+), 168 deletions(-)
 delete mode 100644 src/main/java/uk/ac/sanger/artemis/io/LineGroupIdGenerator.java
 delete mode 100644 src/test/java/uk/ac/sanger/artemis/io/LineGroupIdGeneratorTest.java

diff --git a/src/main/java/uk/ac/sanger/artemis/io/LineGroup.java b/src/main/java/uk/ac/sanger/artemis/io/LineGroup.java
index 9057d25f6..5c9b3ae71 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/LineGroup.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/LineGroup.java
@@ -156,9 +156,6 @@ public abstract class LineGroup
     genbank_hash.put ("CONTIG","CONTIG");
   }
   
-  /** A unique ID for this line group */
-  private Long uniqueId = LineGroupIdGenerator.createID();
-
   /**
    *  Try to read and return a new LineGroup object from a stream.
    *  @param reader The stream to read from.
@@ -471,13 +468,4 @@ public static void writeStartOfGFFEntry (Writer writer) throws IOException
   public abstract void writeToStream (final Writer out_stream)
       throws IOException;
 
-  /**
-   * Return the line group's unique ID.
-   * @return Long
-   */
-  public Long getUniqueId()
-  {
-	return uniqueId;
-  }
-
 }
diff --git a/src/main/java/uk/ac/sanger/artemis/io/LineGroupCache.java b/src/main/java/uk/ac/sanger/artemis/io/LineGroupCache.java
index 5413a2691..73b2ca531 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/LineGroupCache.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/LineGroupCache.java
@@ -3,7 +3,6 @@
 import java.io.IOException;
 import java.io.Writer;
 import java.util.ArrayList;
-import java.util.LinkedHashMap;
 import java.util.List;
 
 /**
@@ -21,9 +20,9 @@ public class LineGroupCache
 	
 	public static final String FORWARD_REF_DELIM = "###";
 	
-	private LinkedHashMap<Long, LineGroup> headers = new LinkedHashMap<Long, LineGroup>();
+	private List<LineGroup> headers = new ArrayList<LineGroup>();
 	
-	private LinkedHashMap<Long, LineGroup> misc = new LinkedHashMap<Long, LineGroup>();
+	private List<LineGroup> misc = new ArrayList<LineGroup>();
 	
 	/**
 	 * The Sequence line group.
@@ -38,9 +37,9 @@ public class LineGroupCache
 	/**
 	 * Determine which internal data structure is associated with the given group.
 	 * @param group LineGroup
-	 * @return LinkedHashMap
+	 * @return List
 	 */
-	protected LinkedHashMap<Long, LineGroup> findRelevantList(LineGroup group)
+	protected List<LineGroup> findRelevantList(LineGroup group)
 	{
 		if (group instanceof FeatureHeader)
 		{
@@ -75,7 +74,7 @@ public LineGroup getSequence()
 	 * 
 	 * @param lineGroup LineGroup
 	 */
-	public synchronized void add(LineGroup lineGroup)
+	public void add(LineGroup lineGroup)
 	{
 		if (lineGroup instanceof FeatureTable)
 		{
@@ -92,16 +91,16 @@ else if (lineGroup instanceof GFFMisc && ((GFFMisc) lineGroup).getLine().startsW
 		}
 		else
 		{
-			findRelevantList(lineGroup).put(lineGroup.getUniqueId(), lineGroup);
+			findRelevantList(lineGroup).add(lineGroup);
 		}
 	}
 
 	/**
-	 * Remove the given line group from this cache and return it.
+	 * Remove the given feature table from this cache and return it.
 	 * @param lineGroup LineGroup
 	 * @return LineGroup
 	 */
-	public synchronized LineGroup remove(LineGroup lineGroup)
+	public LineGroup removeFeatureTable(LineGroup lineGroup)
 	{	
 		LineGroup group = null;
 		
@@ -111,15 +110,24 @@ public synchronized LineGroup remove(LineGroup lineGroup)
 			featureTable = null;
 			
 		}
-		else if (lineGroup instanceof Sequence)
+		
+		return group;
+	}
+	
+	/**
+	 * Remove the given sequence from this cache and return it.
+	 * @param lineGroup LineGroup
+	 * @return LineGroup
+	 */
+	public LineGroup removeSequence(LineGroup lineGroup)
+	{	
+		LineGroup group = null;
+		
+		if (lineGroup instanceof Sequence)
 		{
 			group = sequence;
 			sequence = null;
 		}
-		else 
-		{
-			group = findRelevantList(lineGroup).remove(lineGroup.getUniqueId());
-		}
 		
 		return group;
 	}
@@ -129,7 +137,7 @@ else if (lineGroup instanceof Sequence)
      *  added to line_groups in the appropriate place.
      *  @return FeatureTable
      **/
-    public synchronized FeatureTable createFeatureTable() 
+    public FeatureTable createFeatureTable() 
     {
     	featureTable = new StreamFeatureTable();
 
@@ -141,14 +149,14 @@ public synchronized FeatureTable createFeatureTable()
      *  header.
      *  @return String
      **/
-    public synchronized String getHeadersAsText() 
+    public String getHeadersAsText() 
     {
       int numLineGroups = headers.size() + misc.size();
       
       List<LineGroup> groups = new ArrayList<LineGroup>(numLineGroups);
       
-      groups.addAll(misc.values());
-      groups.addAll(headers.values());
+      groups.addAll(misc);
+      groups.addAll(headers);
       
       final StringBuilder buffer = 
     		  new StringBuilder( numLineGroups*80 );
@@ -168,12 +176,11 @@ public synchronized String getHeadersAsText()
      * Return a list of all line groups in the cache.
      * @return List
      */
-    public synchronized List<LineGroup> getAllLineGroups()
+    public List<LineGroup> getAllLineGroups()
     {
     	List<LineGroup> allGroups = new ArrayList<LineGroup>();
-    	allGroups.addAll(misc.values());
-    	allGroups.addAll(headers.values());
-    	//allGroups.addAll(features.values());
+    	allGroups.addAll(misc);
+    	allGroups.addAll(headers);
     	
     	if (featureTable != null)
     		allGroups.add(featureTable);
@@ -188,7 +195,7 @@ public synchronized List<LineGroup> getAllLineGroups()
     /**
      * Clear all miscellaneous line groups.
      */
-    public synchronized void clearMiscLineGroups()
+    public void clearMiscLineGroups()
     {
     	misc.clear();
     }
@@ -196,7 +203,7 @@ public synchronized void clearMiscLineGroups()
     /**
      * Clear the cache.
      */
-    public synchronized void clear()
+    public void clear()
     {
     	headers.clear();
     	misc.clear();
@@ -216,15 +223,15 @@ public void writeToStream(final Entry entry, final Writer writer) throws IOExcep
     	boolean hasSequence = false;
     	boolean hasFeatureTable = false;
     	
-    	for (java.util.Map.Entry<Long, LineGroup> miscGroup : misc.entrySet())
+    	for (LineGroup miscGroup : misc)
     	{
-    		miscGroup.getValue().writeToStream(writer);
+    		miscGroup.writeToStream(writer);
     		++num;
     	}
     	
-    	for (java.util.Map.Entry<Long, LineGroup> headerGroup : headers.entrySet())
+    	for (LineGroup headerGroup : headers)
         {
-        	headerGroup.getValue().writeToStream(writer);
+        	headerGroup.writeToStream(writer);
         	++num;
         }
     	
diff --git a/src/main/java/uk/ac/sanger/artemis/io/LineGroupIdGenerator.java b/src/main/java/uk/ac/sanger/artemis/io/LineGroupIdGenerator.java
deleted file mode 100644
index 9394366d9..000000000
--- a/src/main/java/uk/ac/sanger/artemis/io/LineGroupIdGenerator.java
+++ /dev/null
@@ -1,28 +0,0 @@
-package uk.ac.sanger.artemis.io;
-
-import java.util.concurrent.atomic.AtomicLong;
-
-/**
- * An ID generator for creating line group 
- * unique IDs. We could use a UUID for this but
- * that's twice the size of a Long!
- * <br/>
- * Thread Safe.
- * 
- * @author kp11
- *
- */
-public class LineGroupIdGenerator
-{
-	/** A counter variable */
-	private static AtomicLong idCounter = new AtomicLong();
-
-	/**
-	 * Increment the counter and return the new value.
-	 * @return Long
-	 */
-	public static Long createID()
-	{
-	    return idCounter.getAndIncrement();
-	}
-}
diff --git a/src/main/java/uk/ac/sanger/artemis/io/SimpleDocumentEntry.java b/src/main/java/uk/ac/sanger/artemis/io/SimpleDocumentEntry.java
index 5ce773b26..45963b32c 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/SimpleDocumentEntry.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/SimpleDocumentEntry.java
@@ -662,7 +662,7 @@ protected boolean removeInternal(Feature feature)
 
         // get rid of the feature table
         if(feature_table.getFeatureCount() == 0) 
-          removeLineGroup(feature_table);
+          removeFeatureTable(feature_table);
 
         setDirtyFlag();
 
@@ -902,12 +902,22 @@ protected FeatureTable getFeatureTable()
   }
 
   /**
-   *  Remove the given LineGroup from this SimpleDocumentEntry.
+   *  Remove the given feature table LineGroup from this SimpleDocumentEntry.
    **/
-  protected void removeLineGroup(final LineGroup line_group) 
+  protected void removeFeatureTable(final FeatureTable ft) 
   {
-	LineGroup group = line_groups.remove(line_group);
-	if (group != null)
+	if (line_groups.removeFeatureTable(ft) != null)
+	{
+	  setDirtyFlag();	
+	}
+  }
+  
+  /**
+   *  Remove the given sequence LineGroup from this SimpleDocumentEntry.
+   **/
+  protected void removeSequence(final StreamSequence seq) 
+  {
+	if (line_groups.removeSequence(seq) != null)
 	{
 	  setDirtyFlag();	
 	}
@@ -1033,7 +1043,7 @@ public void setSequence(final StreamSequence sequence)
     final Sequence old_sequence = getSequence();
 
     if(old_sequence != null) 
-      removeLineGroup((StreamSequence) old_sequence);
+      removeSequence((StreamSequence) old_sequence);
 
     addLineGroup(sequence);
   }
diff --git a/src/test/java/uk/ac/sanger/artemis/io/LineGroupCacheTest.java b/src/test/java/uk/ac/sanger/artemis/io/LineGroupCacheTest.java
index 26f6b580c..831362733 100644
--- a/src/test/java/uk/ac/sanger/artemis/io/LineGroupCacheTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/io/LineGroupCacheTest.java
@@ -24,7 +24,6 @@
 
 import java.io.StringReader;
 import java.io.StringWriter;
-import java.util.LinkedHashMap;
 import java.util.List;
 
 import org.junit.After;
@@ -142,18 +141,12 @@ public void testFindRelevantList() throws Exception
 	{
 		// When
 		
-		LinkedHashMap<Long, LineGroup> result = groups.findRelevantList(hdr1);
+		List<LineGroup> result = groups.findRelevantList(hdr1);
 		
 		// Then
 		
-		assertNotNull( hdr1.getUniqueId() );
-		assertTrue( hdr1.getUniqueId() >= 0 );
 		assertNotNull( result );
 		assertEquals( NUM_EMBL_HDRS_FOR_TESTS, result.size() );
-		assertEquals( hdr1, result.get(hdr1.getUniqueId()) );
-		assertNotNull( hdr2.getUniqueId() );
-		assertTrue( hdr2.getUniqueId() >= 0 );
-		assertEquals( hdr2, result.get(hdr2.getUniqueId()) );
 		
 		// When
 		
@@ -161,8 +154,6 @@ public void testFindRelevantList() throws Exception
 		
 		// Then
 		
-		assertNotNull( misc1.getUniqueId() );
-		assertTrue( misc1.getUniqueId() >= 0 );
 		assertNotNull( result );
 		assertEquals( NUM_EMBL_MISC_FEATURES_FOR_TESTS, result.size() );
 		
@@ -227,55 +218,33 @@ public void testAdd() throws Exception
 	}
 	
 	/**
-	 * Test the remove() method.
+	 * Test the testRemoveFeatureTable() method.
 	 * @throws Exception
 	 */
 	@Test
-	public void testRemove() throws Exception
-	{	
+	public void testRemoveFeatureTable() throws Exception
+	{					
 		// When
 		
-		LineGroup removedHdr1 = groups.remove(hdr1);
-		
-		// Then
-		
-		assertNotNull( removedHdr1 );
-		assertEquals( hdr1, removedHdr1 );
-		assertEquals( NUM_EMBL_HDRS_FOR_TESTS-1, groups.findRelevantList(hdr2).size() );
-		
-		// When
-		
-		LineGroup removedHdr2 = groups.remove(hdr2);
-		
-		// Then
-		
-		assertNotNull( removedHdr2 );
-		assertEquals( hdr2, removedHdr2 );
-		assertEquals( NUM_EMBL_HDRS_FOR_TESTS-2, groups.findRelevantList(hdr2).size() );
-				
-		// When
-		
-		LineGroup removedMisc1 = groups.remove(misc1);
-		
-		// Then
-		
-		assertNotNull( removedMisc1 );
-		assertEquals( misc1, removedMisc1 );
-		assertEquals( NUM_EMBL_MISC_FEATURES_FOR_TESTS-1, groups.findRelevantList(misc1).size() );
-				
-		// When
-		
-		LineGroup removedFT = groups.remove(ft);
+		LineGroup removedFT = groups.removeFeatureTable(ft);
 		
 		// Then
 		
 		assertNotNull( removedFT );
 		assertEquals( ft, removedFT );
 		assertNull( groups.getFeatureTable() );
-		
+	}
+	
+	/**
+	 * Test the testRemoveSequence() method.
+	 * @throws Exception
+	 */
+	@Test
+	public void testRemoveSequence() throws Exception
+	{
 		// When
 		
-		LineGroup removedSeq = groups.remove(sequence);
+		LineGroup removedSeq = groups.removeSequence(sequence);
 		
 		// Then
 		
@@ -352,6 +321,9 @@ public void testClear()
 		// Check multiple calls
 		groups.clear();
 		groups.clear();
+		
+		assertNull(groups.getFeatureTable());
+		assertNull(groups.getSequence());
 	}
 	
 	/**
diff --git a/src/test/java/uk/ac/sanger/artemis/io/LineGroupIdGeneratorTest.java b/src/test/java/uk/ac/sanger/artemis/io/LineGroupIdGeneratorTest.java
deleted file mode 100644
index ab33d8351..000000000
--- a/src/test/java/uk/ac/sanger/artemis/io/LineGroupIdGeneratorTest.java
+++ /dev/null
@@ -1,48 +0,0 @@
-/* 
- * This file is part of Artemis
- *
- * Copyright (C) 2019  Genome Research Limited
- *
- * This program is free software; you can redistribute it and/or
- * modify it under the terms of the GNU General Public License
- * as published by the Free Software Foundation; either version 2
- * of the License, or (at your option) any later version.
- *
- * This program is distributed in the hope that it will be useful,
- * but WITHOUT ANY WARRANTY; without even the implied warranty of
- * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
- * GNU General Public License for more details.
- *
- * You should have received a copy of the GNU General Public License
- * along with this program; if not, write to the Free Software
- * Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA  02111-1307, USA.
- *
- */
-package uk.ac.sanger.artemis.io;
-
-import static org.junit.Assert.assertEquals;
-
-import org.junit.Test;
-
-/**
- * Unit test for the LineGroupIdGenerator class.
- * 
- * @author kp11
- *
- */
-public class LineGroupIdGeneratorTest
-{
-
-	/**
-	 * Test the createID() method.
-	 */
-	@Test
-	public void testCreateID()
-	{
-		for (long i = 0L; i < 1000L; i++)
-		{
-			assertEquals(i, LineGroupIdGenerator.createID().longValue());
-		}
-	}
-	
-}
diff --git a/src/test/java/uk/ac/sanger/artemis/io/SimpleDocumentEntryTest.java b/src/test/java/uk/ac/sanger/artemis/io/SimpleDocumentEntryTest.java
index 4d4e9b519..054e7d9cb 100644
--- a/src/test/java/uk/ac/sanger/artemis/io/SimpleDocumentEntryTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/io/SimpleDocumentEntryTest.java
@@ -189,6 +189,12 @@ public class SimpleDocumentEntryTest
 	
 	@Mock
 	private FileDocument saveToDoc;
+	@Mock
+	private EntryInformation entryInfo;
+	@Mock
+	private StreamSequence seq;
+	@Mock
+	private FeatureTable ft;
 	
 	@Before
 	public void setUp() throws Exception {
@@ -1205,4 +1211,50 @@ public void testBlast() throws Exception
 		assertEquals( 174, nextFeature.getFirstBase() );
 		assertEquals( 181, nextFeature.getLastBase() );
 	}
+	
+	/**
+	 * Test the removeFeatureTable method.
+	 */
+	@Test
+	public void testRemoveFeatureTable() 
+	{
+		// Given
+		
+		SimpleDocumentEntry entry = new GFFDocumentEntry(entryInfo);
+		entry.addLineGroup(ft);
+		
+		assertNotNull(entry.getFeatureTable());
+		assertEquals(ft, entry.getFeatureTable());
+		
+		// When
+		
+		entry.removeFeatureTable(ft);
+		
+		// Then
+		
+		assertNull(entry.line_groups.getFeatureTable());
+	}
+	
+	/**
+	 * Test the removeSequence method.
+	 */
+	@Test
+	public void testRemoveSequence() 
+	{
+		// Given
+		
+		SimpleDocumentEntry entry = new GFFDocumentEntry(entryInfo);
+		entry.addLineGroup(seq);
+		
+		assertNotNull(entry.getSequence());
+		assertEquals(seq, entry.getSequence());
+		
+		// When
+		
+		entry.removeSequence(seq);
+		
+		// Then
+		
+		assertNull(entry.line_groups.getSequence());
+	}
 }

From 47a3f0c1cbfe513f12cf8c53fab2d8fa2310b3aa Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Thu, 17 Oct 2019 17:22:08 +0100
Subject: [PATCH 16/25] Code tidy ups and unit tests

---
 etc/versions                                  |   8 +-
 pom.xml                                       |   2 +-
 .../uk/ac/sanger/artemis/FeatureSegment.java  |  24 +-
 .../uk/ac/sanger/artemis/io/Location.java     |   7 +-
 .../artemis/io/SimpleDocumentFeature.java     |  12 +-
 .../uk/ac/sanger/artemis/sequence/Strand.java |  46 ++-
 .../artemis/util/LinePushBackReader.java      |   3 +-
 .../ac/sanger/artemis/FeatureSegmentTest.java | 222 +++++++++++++++
 .../uk/ac/sanger/artemis/io/LocationTest.java | 156 +++++++++++
 .../sanger/artemis/sequence/StrandTest.java   | 265 ++++++++++++++++++
 10 files changed, 693 insertions(+), 52 deletions(-)
 create mode 100644 src/test/java/uk/ac/sanger/artemis/FeatureSegmentTest.java
 create mode 100644 src/test/java/uk/ac/sanger/artemis/io/LocationTest.java
 create mode 100644 src/test/java/uk/ac/sanger/artemis/sequence/StrandTest.java

diff --git a/etc/versions b/etc/versions
index 66c183497..0c91a7e9f 100644
--- a/etc/versions
+++ b/etc/versions
@@ -1,4 +1,4 @@
-Artemis Release 18.0.3
-ACT Release 18.0.3
-DNAPlotter Release 18.0.3
-BamView 18.0.3
\ No newline at end of file
+Artemis Release 18.1.0
+ACT Release 18.1.0
+DNAPlotter Release 18.1.0
+BamView 18.1.0
\ No newline at end of file
diff --git a/pom.xml b/pom.xml
index 5b349ddb2..d37543126 100644
--- a/pom.xml
+++ b/pom.xml
@@ -5,7 +5,7 @@
 	<modelVersion>4.0.0</modelVersion>
 	<groupId>uk.ac.sanger</groupId>
 	<artifactId>artemis</artifactId>
-	<version>18.0.3</version>
+	<version>18.1.0</version>
 	
 	<name>Artemis</name>
 	<description>Artemis is a free genome browser and annotation tool that allows visualisation of sequence features, next generation data and the results of analyses within the context of the sequence, and also its six-frame translation.
diff --git a/src/main/java/uk/ac/sanger/artemis/FeatureSegment.java b/src/main/java/uk/ac/sanger/artemis/FeatureSegment.java
index cdfad1425..e46a55896 100644
--- a/src/main/java/uk/ac/sanger/artemis/FeatureSegment.java
+++ b/src/main/java/uk/ac/sanger/artemis/FeatureSegment.java
@@ -189,7 +189,7 @@ public Marker getEnd () {
    **/
   void setStartPosition (final int position)
       throws OutOfRangeException {
-    getStart ().setPosition (position);
+    start.setPosition (position);
     updateRange ();
   }
 
@@ -198,7 +198,7 @@ void setStartPosition (final int position)
    **/
   void setEndPosition (final int position)
       throws OutOfRangeException {
-    getEnd ().setPosition (position);
+    end.setPosition (position);
     updateRange ();
   }
 
@@ -216,8 +216,8 @@ public Range getRawRange () {
   public MarkerRange getMarkerRange () {
     try {
       return new MarkerRange (getFeature ().getStrand (),
-                              getStart ().getPosition (),
-                              getEnd ().getPosition ());
+                              start.getPosition (),
+                              end.getPosition ());
     } catch (OutOfRangeException e) {
       throw new Error ("internal error - " + e);
     }
@@ -236,7 +236,7 @@ public void removeFromFeature ()
    *  Return the number of bases in this feature (total of all segments).
    **/
   public int getBaseCount () {
-    return getEnd ().getPosition () - getStart ().getPosition () + 1;
+    return end.getPosition () - start.getPosition () + 1;
   }
 
   /**
@@ -245,8 +245,8 @@ public int getBaseCount () {
   public String getBases () {
     final Strand strand = getFeature ().getStrand ();
     try {
-      return strand.getSubSequence (new Range (getStart ().getPosition (),
-                                               getEnd ().getPosition ()));
+      return strand.getSubSequence (new Range (start.getPosition (),
+                                               end.getPosition ()));
     } catch (OutOfRangeException e) {
       throw new Error ("internal error - unexpected exception: " + e);
     }
@@ -306,11 +306,11 @@ private void updateRange () {
 
     try {
       if (getFeature ().isForwardFeature ()) {
-        new_range = range.change (getStart ().getRawPosition (),
-                                  getEnd ().getRawPosition ());
+        new_range = range.change (start.getRawPosition (),
+                                  end.getRawPosition ());
       } else {
-        new_range = range.change (getEnd ().getRawPosition (),
-                                  getStart ().getRawPosition ());
+        new_range = range.change (end.getRawPosition (),
+                                  start.getRawPosition ());
       }
 
       range = new_range;
@@ -440,7 +440,7 @@ else if(this_direction != direction)
   public int getFrameID () {
     // this will be 0, 1 or 2 depending on which frame the segment is in
     final int start_base_modulo =
-      (getStart ().getPosition () - 1 + getFrameShift ()) % 3;
+      (start.getPosition () - 1 + getFrameShift ()) % 3;
 
 //  if (getFeature ().getStrand ().isForwardStrand ()) 
     if(isForwardSegment())
diff --git a/src/main/java/uk/ac/sanger/artemis/io/Location.java b/src/main/java/uk/ac/sanger/artemis/io/Location.java
index b479348b8..80d043f49 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/Location.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/Location.java
@@ -322,15 +322,16 @@ public String toStringShort()
    **/
   public boolean isComplement() 
   {
-    if(getParsedLocation ().getType () == LocationParseNode.COMPLEMENT) 
+	int type = getParsedLocation ().getType ();
+	
+    if(type == LocationParseNode.COMPLEMENT) 
     {
       // COMPLEMENT(RANGE)
       return true;
     }
     else 
     {
-      if(getParsedLocation().getType() == LocationParseNode.JOIN ||
-         getParsedLocation().getType() == LocationParseNode.ORDER) 
+      if(type == LocationParseNode.JOIN || type == LocationParseNode.ORDER) 
       {
         // a join/order must have at least one child node
         if(getParsedLocation().getChildren().elementAt(0).getType() ==
diff --git a/src/main/java/uk/ac/sanger/artemis/io/SimpleDocumentFeature.java b/src/main/java/uk/ac/sanger/artemis/io/SimpleDocumentFeature.java
index 3c8f51454..e5e03a504 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/SimpleDocumentFeature.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/SimpleDocumentFeature.java
@@ -362,7 +362,7 @@ public void setQualifier (final Qualifier qualifier)
       throw new ReadOnlyException ();
     }
 
-    getQualifiers ().setQualifier (qualifier);
+    this.qualifiers.setQualifier (qualifier);
 
     setDirtyFlag ();
   }
@@ -380,7 +380,7 @@ public void removeQualifierByName (final String name)
       throw new ReadOnlyException ();
     }
 
-    getQualifiers ().removeQualifierByName (name);
+    this.qualifiers.removeQualifierByName (name);
 
     setDirtyFlag ();
   }
@@ -401,7 +401,7 @@ public Qualifier addQualifierValues (final Qualifier qualifier)
 
     setDirtyFlag ();
 
-    return getQualifiers ().addQualifierValues (qualifier);
+    return this.qualifiers.addQualifierValues (qualifier);
   }
 
   /**
@@ -430,7 +430,7 @@ public Location getLocation () {
    **/
   public int getFirstBase () {
     if (first_base == -1) {
-      first_base = getLocation ().getFirstBase ();
+      first_base = location.getFirstBase ();
     }
     return first_base;
   }
@@ -440,7 +440,7 @@ public int getFirstBase () {
    **/
   public int getLastBase () {
     if (last_base == -1) {
-      last_base = getLocation ().getLastBase ();
+      last_base = location.getLastBase ();
     }
     return last_base;
   }
@@ -459,7 +459,7 @@ public QualifierVector getQualifiers () {
    *  there no such Qualifier.
    **/
   public Qualifier getQualifierByName (String name) {
-    return getQualifiers ().getQualifierByName (name);
+    return this.qualifiers.getQualifierByName (name);
   }
 
 
diff --git a/src/main/java/uk/ac/sanger/artemis/sequence/Strand.java b/src/main/java/uk/ac/sanger/artemis/sequence/Strand.java
index a6e213296..ba1df8b0e 100644
--- a/src/main/java/uk/ac/sanger/artemis/sequence/Strand.java
+++ b/src/main/java/uk/ac/sanger/artemis/sequence/Strand.java
@@ -80,9 +80,9 @@ public Bases getBases () {
    **/
   public String getStrandBases () {
     if (getDirection () == FORWARD) {
-      return getBases ().toString ();
+      return bases.toString ();
     } else {
-      return Bases.reverseComplement (getBases ().toString ());
+      return Bases.reverseComplement (bases.toString ());
     }
   }
 
@@ -90,7 +90,7 @@ public String getStrandBases () {
    *  Return the direction of this strand.
    **/
   public int getDirection () {
-    if (getBases ().getForwardStrand () == this) {
+    if (bases.getForwardStrand () == this) {
       return FORWARD;
     } else {
       return REVERSE;
@@ -112,7 +112,7 @@ public boolean isForwardStrand () {
    *  Return the length of this Strand in bases.
    **/
   public int getSequenceLength () {
-    return getBases ().getLength ();
+    return bases.getLength ();
   }
 
   /**
@@ -499,9 +499,7 @@ private static Marker getEndOfORF (final Marker search_marker,
    **/
   public Marker makeMarker (int position)
       throws OutOfRangeException {
-    final Marker new_marker = new Marker (this, position);
-
-    return new_marker;
+    return new Marker (this, position);
   }
 
   /**
@@ -514,7 +512,7 @@ public Marker makeMarkerFromRawPosition (int position)
     if (getDirection () == FORWARD) {
       return makeMarker (position);
     } else {
-      return makeMarker (getBases ().getComplementPosition (position));
+      return makeMarker (bases.getComplementPosition (position));
     }
   }
 
@@ -539,9 +537,9 @@ public MarkerRange makeMarkerRangeFromRawPositions (int raw_start_position,
       return new MarkerRange (this, raw_start_position, raw_end_position);
     } else {
       final int real_start_position =
-        getBases ().getComplementPosition (raw_start_position);
+        bases.getComplementPosition (raw_start_position);
       final int real_end_position =
-        getBases ().getComplementPosition (raw_end_position);
+        bases.getComplementPosition (raw_end_position);
       return new MarkerRange (this, real_start_position, real_end_position);
     }
   }
@@ -550,7 +548,7 @@ public MarkerRange makeMarkerRangeFromRawPositions (int raw_start_position,
    *  Return the position on the Bases object of the argument base.
    **/
   public int getRawPosition (int strand_position) {
-    return getBases ().getRawPosition (strand_position, getDirection ());
+    return bases.getRawPosition (strand_position, getDirection ());
   }
 
   /**
@@ -596,12 +594,12 @@ public static void addBases (final Marker position,
    **/
   public AminoAcidSequence getTranslation(final Range range,
                                           final boolean unknown_is_x) {
-    return getBases ().getTranslation (range, getDirection (), unknown_is_x);
+    return bases.getTranslation (range, getDirection (), unknown_is_x);
   }
 
   public AminoAcidSequence getSpacedTranslation(final Range range,
                                           final boolean unknown_is_x) {
-    return getBases ().getSpacedTranslation (range, getDirection (), unknown_is_x);
+    return bases.getSpacedTranslation (range, getDirection (), unknown_is_x);
   }
 
 
@@ -610,7 +608,7 @@ public AminoAcidSequence getSpacedTranslation(final Range range,
    *  @param range The inclusive range of bases to return.
    **/
   public String getSubSequence (Range range) {
-    return getBases ().getSubSequence (range, getDirection ());
+    return bases.getSubSequence (range, getDirection ());
   }
 
   /**
@@ -619,7 +617,7 @@ public String getSubSequence (Range range) {
    *  @param range The inclusive range of bases to return.
    **/
   public char[] getRawSubSequenceC (Range range) {
-    return getBases ().getSubSequenceC (range, FORWARD);
+    return bases.getSubSequenceC (range, FORWARD);
   }
   
   /**
@@ -629,7 +627,7 @@ public char[] getRawSubSequenceC (Range range) {
    *  @param range The inclusive range of bases to return.
    **/
   public String getRawSubSequence (Range range) {
-    return getBases ().getSubSequence (range, FORWARD);
+    return bases.getSubSequence (range, FORWARD);
   }
 
   /**
@@ -644,9 +642,9 @@ public static String markerRangeBases (final MarkerRange marker_range) {
    **/
   public int getACount () {
     if (isForwardStrand ()) {
-      return getBases ().getACount ();
+      return bases.getACount ();
     } else {
-      return getBases ().getTCount ();
+      return bases.getTCount ();
     }
   }
 
@@ -655,9 +653,9 @@ public int getACount () {
    **/
   public int getTCount () {
     if (isForwardStrand ()) {
-      return getBases ().getTCount ();
+      return bases.getTCount ();
     } else {
-      return getBases ().getACount ();
+      return bases.getACount ();
     }
   }
 
@@ -666,9 +664,9 @@ public int getTCount () {
    **/
   public int getGCount () {
     if (isForwardStrand ()) {
-      return getBases ().getGCount ();
+      return bases.getGCount ();
     } else {
-      return getBases ().getCCount ();
+      return bases.getCCount ();
     }
   }
 
@@ -677,9 +675,9 @@ public int getGCount () {
    **/
   public int getCCount () {
     if (isForwardStrand ()) {
-      return getBases ().getCCount ();
+      return bases.getCCount ();
     } else {
-      return getBases ().getGCount ();
+      return bases.getGCount ();
     }
   }
 
diff --git a/src/main/java/uk/ac/sanger/artemis/util/LinePushBackReader.java b/src/main/java/uk/ac/sanger/artemis/util/LinePushBackReader.java
index c2a6e33b7..c686b2eb4 100644
--- a/src/main/java/uk/ac/sanger/artemis/util/LinePushBackReader.java
+++ b/src/main/java/uk/ac/sanger/artemis/util/LinePushBackReader.java
@@ -61,8 +61,7 @@ public String readLine () throws IOException {
       line_buffer = null;
       return tmp_string;
     } else {
-      String line = buffered_reader.readLine ();
-      return line;
+      return buffered_reader.readLine ();
     }
   }
 
diff --git a/src/test/java/uk/ac/sanger/artemis/FeatureSegmentTest.java b/src/test/java/uk/ac/sanger/artemis/FeatureSegmentTest.java
new file mode 100644
index 000000000..0f93ecdbf
--- /dev/null
+++ b/src/test/java/uk/ac/sanger/artemis/FeatureSegmentTest.java
@@ -0,0 +1,222 @@
+package uk.ac.sanger.artemis;
+
+import static org.junit.Assert.*;
+
+import org.junit.After;
+import org.junit.Before;
+import org.junit.Test;
+import org.mockito.Mock;
+import org.mockito.MockitoAnnotations;
+
+import static org.mockito.Mockito.*;
+
+import uk.ac.sanger.artemis.io.EmblStreamSequence;
+import uk.ac.sanger.artemis.io.Location;
+import uk.ac.sanger.artemis.io.LocationParseException;
+import uk.ac.sanger.artemis.io.Range;
+import uk.ac.sanger.artemis.sequence.Bases;
+import uk.ac.sanger.artemis.sequence.Strand;
+import uk.ac.sanger.artemis.util.OutOfRangeException;
+
+/**
+ * Unit tests for the FeatureSegment class.
+ * 
+ * @author kp11
+ *
+ */
+public class FeatureSegmentTest
+{
+
+	private static final int START_POS = 70;
+	private static final int END_POS   = 80;
+	
+	private static final int FEATURE_START_POS = 10;
+	private static final int FEATURE_END_POS   = 150;
+	
+	// NUM_SEQ_BASES bases...
+	private static final String SEQUENCE = "ggcgaggcggggaaagcactgcgcgctgacggtggtgctgattgtattttttcagcgtctcagcgcgtcgtgacggcacttagtctgcccgttgaggcgttgtgtgtctgcggggtgttttgtgcggtggtgagcgtgtgaggggggatgacggggtgtaaaaaagccgcccgcaggcggcgatgttcagtcgttgtcagtgtccagtgagtagtttttaaagcggatgacctcctgaccgagccagccgtttatctcgcggatcctgtcctgtaacgggataagctcattgcggacaaagacctttgccactttctcaatatcacccagcgacccgacgttctccggcttgccacccatcaactgaaaggggatgcggtgcgcgtccagcaggtcagcggcgctggcttttttgatattaaaaaaatcgtccttcgtcgccacttcactgagggggataattttaatgccgtcggctttcccctgtggggcatagagaaacaggtttttaaagttgttgcggcctttcgacttgaccatgttttcgcgaagcatttcgatatcgttgcgatcctgcacggcatcggtgacatacatgatgtatccggcatgtgcgccattttcgtaatacttgcggcggaacaacgtggccgactcattcagccaggcagagttaagggcgctgagatattccggcaggccgtacagctcctgattaatatccggctccagcaggtgaaacacggagccgggcgcgaaggctgtcggctcgttgaaggacggcacccaccagtaaacatcctcttccacgccacggcgggtatattttgccggtgaggtttccagtctgatgaccttaccggtggtgctgtaacgcttttccagaaacgcattaccgaacaccagaaaatccagcacaaagcggctgaaatcctgctgggaaagccatggatgcgggataaatgtcgaggccagaatattgcgtttgacgtaaatcggcgagctgtgatgcacggcagcccgcaggctttttgccagaccggtaaagctgaccggtggctcataccatctgccgttactgatgcactcgacgtaatccagaatgtcacggcggtcgagtaccggcaccggctcaccaaaggtgaatgcctccattttcgggccgctggcggtcattgtttttgccgcaggttgcggtgttttcccttttttcttgctcatcagtaaaactccagaatggtggatgtcagcggggtgctgataccggcggtgagtggctcatttaacagggcgtgcatggtcgcccaggcgaggtcggcgtggctggcttcctcgctgcggctggcctcataggtggcgctgcgtccgctgctggtcatggtcttgcggatagccataaacgagctggtgatgtcggtggcgctgacgtcgtattccagacagccacggcggataacgtcttttgccttgagcaccattgcggttttcatttccggcgtgtagcggatatcacgcgcggcgggatagaacgagcgcacgagctggaacacgccgacaccgaggccggtggcatcaataccgatgtattcgacgttgtatttttcggtgagtttgcggatggattccgcctgggtggcaaagtccatgcctttccactggtgacgctcaagtattctgaatttgccaccggccaccaccggcggtgccagtaccacgcatccggcgctgtcgccacggtgtgacgggtcgtaaccaatccataccgggcgggagccgaacggattggcggcaaacggcgcatagtcttcccattcttccagcgtgtcgaccatgcagcgttgcagctcctcgaacgggaacaccgatgccttgtcgtcaacaaattcacacatgaacaggtttttaaaatcgtcggcgctgttttcgcgt";
+	
+	@Mock
+	private Feature feature;
+	
+	private Range range;
+	private Bases bases;
+	private Strand fwdStrand;
+	
+	
+	@Before
+	public void setupForTest() throws Exception
+	{
+		MockitoAnnotations.initMocks(this);
+		
+		range = new Range (START_POS, END_POS);
+		bases = new Bases( new EmblStreamSequence(SEQUENCE) );
+		fwdStrand = bases.getForwardStrand();
+	}
+	
+	@After
+	public void cleanupAfterTest() throws Exception
+	{
+		range = null;
+		bases = null;
+		fwdStrand = null;
+	}
+	
+	@Test
+	public void testSetStartPosition() throws OutOfRangeException, LocationParseException
+	{
+		// When
+
+		when(feature.getStrand()).thenReturn(fwdStrand);
+		when(feature.getLocation()).thenReturn(new Location(new Range(FEATURE_START_POS, FEATURE_END_POS)));
+		when(feature.isForwardFeature()).thenReturn(true);
+		
+		FeatureSegment segment = new FeatureSegment(
+				feature,
+                range);
+		
+		// Sanity check
+		assertEquals(START_POS, segment.getStart().getPosition());
+		assertEquals(END_POS, segment.getEnd().getPosition());
+		
+		
+		try
+		{
+			segment.setStartPosition(50);
+		}
+		catch (OutOfRangeException e)
+		{
+			fail("setStartPosition threw unexpected OutOfRangeException exeption: " + e.getMessage());
+		}
+		
+		// Then (also intrinsically test updateRange)
+		
+		assertEquals(50, segment.getStart().getPosition());
+		assertEquals(50, segment.getRawRange().getStart());
+		assertEquals(END_POS, segment.getRawRange().getEnd());
+	}
+	
+	@Test
+	public void testSetEndPosition() throws OutOfRangeException, LocationParseException
+	{
+		// When
+
+		when(feature.getStrand()).thenReturn(fwdStrand);
+		when(feature.getLocation()).thenReturn(new Location(new Range(FEATURE_START_POS, FEATURE_END_POS)));
+		when(feature.isForwardFeature()).thenReturn(true);
+		
+		FeatureSegment segment = new FeatureSegment(
+				feature,
+                range);
+		
+		// Sanity check
+		assertEquals(START_POS, segment.getStart().getPosition());
+		assertEquals(END_POS, segment.getEnd().getPosition());
+		
+		try
+		{
+			segment.setEndPosition(100);
+		}
+		catch (OutOfRangeException e)
+		{
+			fail("setEndPosition threw unexpected OutOfRangeException exeption: " + e.getMessage());
+		}
+		
+		// Then (also intrinsically test updateRange)
+		
+		assertEquals(100, segment.getEnd().getPosition());
+		assertEquals(START_POS, segment.getRawRange().getStart());
+		assertEquals(100, segment.getRawRange().getEnd());
+	}
+	
+	@Test
+	public void testGetMarkerRange() throws OutOfRangeException
+	{
+		// When
+
+		when(feature.getStrand()).thenReturn(fwdStrand);
+		when(feature.getLocation()).thenReturn(new Location(new Range(FEATURE_START_POS, FEATURE_END_POS)));
+		when(feature.isForwardFeature()).thenReturn(true);
+		
+		FeatureSegment segment = new FeatureSegment(
+				feature,
+                range);
+
+		
+		// Then 
+		
+		assertNotNull(segment.getMarkerRange());
+		assertEquals(START_POS, segment.getMarkerRange().getStart().getRawPosition());
+		assertEquals(END_POS, segment.getMarkerRange().getEnd().getRawPosition());
+		assertEquals(START_POS, segment.getMarkerRange().getStart().getPosition());
+		assertEquals(END_POS, segment.getMarkerRange().getEnd().getPosition());
+	}
+	
+	@Test
+	public void testGetBaseCount() throws OutOfRangeException 
+	{
+		// When
+
+		when(feature.getStrand()).thenReturn(fwdStrand);
+		when(feature.getLocation()).thenReturn(new Location(new Range(FEATURE_START_POS, FEATURE_END_POS)));
+		when(feature.isForwardFeature()).thenReturn(true);
+		
+		FeatureSegment segment = new FeatureSegment(
+				feature,
+                range);
+
+		
+		// Then
+		
+		assertEquals(END_POS-START_POS+1, segment.getBaseCount());
+	}
+	
+	@Test
+	public void testGetBases() throws OutOfRangeException
+	{
+		// When
+
+		when(feature.getStrand()).thenReturn(fwdStrand);
+		when(feature.getLocation()).thenReturn(new Location(new Range(FEATURE_START_POS, FEATURE_END_POS)));
+		when(feature.isForwardFeature()).thenReturn(true);
+		
+		FeatureSegment segment = new FeatureSegment(
+				feature,
+                range);
+
+		
+		// Then
+
+		assertEquals("gtgacggcact", segment.getBases());
+		
+	}
+	
+	@Test
+	public void testGetFrameID() throws OutOfRangeException
+	{
+		// Given
+		
+		FeatureSegmentVector segs = new FeatureSegmentVector();
+		
+		// When
+
+		when(feature.getStrand()).thenReturn(fwdStrand);
+		when(feature.getLocation()).thenReturn(new Location(new Range(FEATURE_START_POS, FEATURE_END_POS)));
+		when(feature.isForwardFeature()).thenReturn(true);
+		
+		FeatureSegment segment = new FeatureSegment(
+				feature,
+                range);
+
+		segs.add(segment);
+		when(feature.getSegments()).thenReturn(segs);
+		
+		int frameID = segment.getFrameID();
+		
+		// Then
+
+		assertEquals(2, frameID);
+		
+	}
+
+}
diff --git a/src/test/java/uk/ac/sanger/artemis/io/LocationTest.java b/src/test/java/uk/ac/sanger/artemis/io/LocationTest.java
new file mode 100644
index 000000000..657a29f7b
--- /dev/null
+++ b/src/test/java/uk/ac/sanger/artemis/io/LocationTest.java
@@ -0,0 +1,156 @@
+/* 
+ * This file is part of Artemis
+ *
+ * Copyright (C) 2019  Genome Research Limited
+ *
+ * This program is free software; you can redistribute it and/or
+ * modify it under the terms of the GNU General Public License
+ * as published by the Free Software Foundation; either version 2
+ * of the License, or (at your option) any later version.
+ *
+ * This program is distributed in the hope that it will be useful,
+ * but WITHOUT ANY WARRANTY; without even the implied warranty of
+ * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+ * GNU General Public License for more details.
+ *
+ * You should have received a copy of the GNU General Public License
+ * along with this program; if not, write to the Free Software
+ * Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA  02111-1307, USA.
+ *
+ */
+package uk.ac.sanger.artemis.io;
+
+import static org.junit.Assert.*;
+
+import org.junit.Test;
+
+
+/**
+ * JUnit test for the Location class.
+ * 
+ * @author kp11
+ *
+ */
+public class LocationTest
+{
+	
+	/**
+	 * Test the isComplement() method.
+	 * @throws Exception
+	 */
+	@Test
+	public void testIsComplement1() throws Exception
+	{
+		// Given
+		Location loc = new Location("complement(3..10)");
+		
+		// When
+		boolean result = loc.isComplement();
+		
+		// Then
+		assertTrue(result);
+	}
+	
+	/**
+	 * Test the isComplement() method.
+	 * @throws Exception
+	 */
+	@Test
+	public void testIsComplement2() throws Exception
+	{
+		// Given
+		Location loc = new Location("join(184264..184375,187655..188211,196610..196906,199841..199927)");
+		
+		// When
+		boolean result = loc.isComplement();
+		
+		// Then
+		assertFalse(result);
+	}
+	
+	/**
+	 * Test the isComplement() method.
+	 * @throws Exception
+	 */
+	@Test
+	public void testIsComplement3() throws Exception
+	{
+		// Given
+		Location loc = new Location("order(3..10)");
+		
+		// When
+		boolean result = loc.isComplement();
+		
+		// Then
+		assertFalse(result);
+	}
+	
+	/**
+	 * Test the isComplement() method.
+	 * @throws Exception
+	 */
+	@Test
+	public void testIsComplement4() throws Exception
+	{
+		// Given
+		Location loc = new Location("join(complement(701966..702762))");
+		
+		// When
+		boolean result = loc.isComplement();
+		
+		// Then
+		assertTrue(result);
+	}
+	
+	/**
+	 * Test the isComplement() method.
+	 * @throws Exception
+	 */
+	@Test
+	public void testIsComplement5() throws Exception
+	{
+		// Given
+		Location loc = new Location("join(complement(249124..251322),complement(242210..243004))");
+		
+		// When
+		boolean result = loc.isComplement();
+		
+		// Then
+		assertTrue(result);
+	}
+	
+	/**
+	 * Test the isComplement() method, for range
+	 * @throws Exception
+	 */
+	@Test
+	public void testIsComplement6() throws Exception
+	{
+		// Given
+		Location loc = new Location("10..30");
+		
+		// When
+		boolean result = loc.isComplement();
+		
+		// Then
+		assertFalse(result);
+	}
+	
+	/**
+	 * Test the isComplement() method, for range
+	 * @throws Exception
+	 */
+	@Test
+	public void testIsComplement7() throws Exception
+	{
+		// Given
+		Location loc = new Location("order(complement(701966..702762))");
+		
+		// When
+		boolean result = loc.isComplement();
+		
+		// Then
+		assertTrue(result);
+	}
+	
+}
diff --git a/src/test/java/uk/ac/sanger/artemis/sequence/StrandTest.java b/src/test/java/uk/ac/sanger/artemis/sequence/StrandTest.java
new file mode 100644
index 000000000..23bf2112a
--- /dev/null
+++ b/src/test/java/uk/ac/sanger/artemis/sequence/StrandTest.java
@@ -0,0 +1,265 @@
+package uk.ac.sanger.artemis.sequence;
+
+import static org.junit.Assert.*;
+
+import org.junit.After;
+import org.junit.Before;
+import org.junit.Test;
+import org.mockito.Mock;
+import org.mockito.MockitoAnnotations;
+
+import static org.mockito.Mockito.*;
+
+import uk.ac.sanger.artemis.Feature;
+import uk.ac.sanger.artemis.io.EmblStreamSequence;
+import uk.ac.sanger.artemis.io.Location;
+import uk.ac.sanger.artemis.io.LocationParseException;
+import uk.ac.sanger.artemis.io.Range;
+import uk.ac.sanger.artemis.sequence.Bases;
+import uk.ac.sanger.artemis.sequence.Strand;
+import uk.ac.sanger.artemis.util.OutOfRangeException;
+
+/**
+ * Basic unit tests for the Strand class.
+ * 
+ * @author kp11
+ *
+ */
+public class StrandTest
+{
+	private static final int FEATURE_START_POS = 10;
+	private static final int FEATURE_END_POS   = 150;
+	
+	// NUM_SEQ_BASES bases...
+	private static final String SEQUENCE = "ggcgaggcggggaaagcactgcgcgctgacggtggtgctgattgtattttttcagcgtctcagcgcgtcgtgacggcacttagtctgcccgttgaggcgttgtgtgtctgcggggtgttttgtgcggtggtgagcgtgtgaggggggatgacggggtgtaaaaaagccgcccgcaggcggcgatgttcagtcgttgtcagtgtccagtgagtagtttttaaagcggatgacctcctgaccgagccagccgtttatctcgcggatcctgtcctgtaacgggataagctcattgcggacaaagacctttgccactttctcaatatcacccagcgacccgacgttctccggcttgccacccatcaactgaaaggggatgcggtgcgcgtccagcaggtcagcggcgctggcttttttgatattaaaaaaatcgtccttcgtcgccacttcactgagggggataattttaatgccgtcggctttcccctgtggggcatagagaaacaggtttttaaagttgttgcggcctttcgacttgaccatgttttcgcgaagcatttcgatatcgttgcgatcctgcacggcatcggtgacatacatgatgtatccggcatgtgcgccattttcgtaatacttgcggcggaacaacgtggccgactcattcagccaggcagagttaagggcgctgagatattccggcaggccgtacagctcctgattaatatccggctccagcaggtgaaacacggagccgggcgcgaaggctgtcggctcgttgaaggacggcacccaccagtaaacatcctcttccacgccacggcgggtatattttgccggtgaggtttccagtctgatgaccttaccggtggtgctgtaacgcttttccagaaacgcattaccgaacaccagaaaatccagcacaaagcggctgaaatcctgctgggaaagccatggatgcgggataaatgtcgaggccagaatattgcgtttgacgtaaatcggcgagctgtgatgcacggcagcccgcaggctttttgccagaccggtaaagctgaccggtggctcataccatctgccgttactgatgcactcgacgtaatccagaatgtcacggcggtcgagtaccggcaccggctcaccaaaggtgaatgcctccattttcgggccgctggcggtcattgtttttgccgcaggttgcggtgttttcccttttttcttgctcatcagtaaaactccagaatggtggatgtcagcggggtgctgataccggcggtgagtggctcatttaacagggcgtgcatggtcgcccaggcgaggtcggcgtggctggcttcctcgctgcggctggcctcataggtggcgctgcgtccgctgctggtcatggtcttgcggatagccataaacgagctggtgatgtcggtggcgctgacgtcgtattccagacagccacggcggataacgtcttttgccttgagcaccattgcggttttcatttccggcgtgtagcggatatcacgcgcggcgggatagaacgagcgcacgagctggaacacgccgacaccgaggccggtggcatcaataccgatgtattcgacgttgtatttttcggtgagtttgcggatggattccgcctgggtggcaaagtccatgcctttccactggtgacgctcaagtattctgaatttgccaccggccaccaccggcggtgccagtaccacgcatccggcgctgtcgccacggtgtgacgggtcgtaaccaatccataccgggcgggagccgaacggattggcggcaaacggcgcatagtcttcccattcttccagcgtgtcgaccatgcagcgttgcagctcctcgaacgggaacaccgatgccttgtcgtcaacaaattcacacatgaacaggtttttaaaatcgtcggcgctgttttcgcgt";
+	
+	@Mock
+	private Feature feature;
+	
+	private Bases bases;
+	private Strand fwdStrand;
+	private Strand revStrand;
+	
+	
+	
+	@Before
+	public void setupForTest() throws Exception
+	{
+		MockitoAnnotations.initMocks(this);
+		
+		bases = new Bases( new EmblStreamSequence(SEQUENCE) );
+		fwdStrand = bases.getForwardStrand();
+		revStrand = bases.getReverseStrand();
+	}
+	
+	@After
+	public void cleanupAfterTest() throws Exception
+	{
+		bases = null;
+		fwdStrand = null;
+		revStrand = null;
+	}
+	
+	@Test
+	public void testGetStrandBases() throws OutOfRangeException, LocationParseException
+	{
+		// When
+
+		when(feature.getStrand()).thenReturn(fwdStrand);
+		when(feature.getLocation()).thenReturn(new Location(new Range(FEATURE_START_POS, FEATURE_END_POS)));
+		when(feature.isForwardFeature()).thenReturn(true);
+		
+		String bases = fwdStrand.getStrandBases();
+		String revBases = revStrand.getStrandBases();
+		
+		// Then
+		
+		assertEquals(SEQUENCE, bases);
+		assertEquals(Bases.reverseComplement(SEQUENCE), revBases);
+	}
+	
+	@Test
+	public void testGetDirection()
+	{
+		
+		assertEquals(Strand.FORWARD, fwdStrand.getDirection());
+		assertEquals(Strand.REVERSE, revStrand.getDirection());
+		
+	}
+	
+	@Test
+	public void testGetSequenceLength()
+	{
+		
+		assertEquals(SEQUENCE.length(), fwdStrand.getSequenceLength());
+		assertEquals(SEQUENCE.length(), revStrand.getSequenceLength());
+		
+	}
+	
+	@Test
+	public void testMakeMarker() throws OutOfRangeException
+	{
+		// When
+		
+		Marker marker1 = fwdStrand.makeMarker(60);
+		Marker marker2 = revStrand.makeMarker(90);
+		
+		// Then
+		
+		assertNotNull(marker1);
+		assertEquals(60, marker1.getRawPosition());
+		assertNotNull(marker2);
+		assertEquals(SEQUENCE.length()-90+1, marker2.getRawPosition());
+		
+	}
+	
+	@Test
+	public void testMakeMarkerFromRawPosition() throws OutOfRangeException
+	{
+		// When
+		
+		Marker marker1 = fwdStrand.makeMarkerFromRawPosition(60);
+		Marker marker2 = revStrand.makeMarkerFromRawPosition(90);
+		
+		// Then
+		
+		assertNotNull(marker1);
+		assertEquals(60, marker1.getRawPosition());
+		assertNotNull(marker2);
+		assertEquals(90, marker2.getRawPosition());
+		
+	}
+	
+	@Test
+	public void testMakeMarkerRangeFromRawPositions() throws OutOfRangeException
+	{
+		// When
+		
+		MarkerRange marker1 = fwdStrand.makeMarkerRangeFromRawPositions(10,30);
+		MarkerRange marker2 = revStrand.makeMarkerRangeFromRawPositions(200,300);
+		
+		// Then
+		
+		assertNotNull(marker1);
+		assertEquals(10, marker1.getRawStart().getPosition());
+		assertEquals(30, marker1.getRawEnd().getPosition());
+		assertNotNull(marker2);
+		assertEquals(SEQUENCE.length()-200+1, marker2.getRawStart().getPosition());
+		assertEquals(SEQUENCE.length()-300+1, marker2.getRawEnd().getPosition());
+		
+	}
+	
+	@Test
+	public void testGetRawPosition()
+	{
+		// When
+
+		int posFwd = fwdStrand.getRawPosition(50);
+		int posRev = revStrand.getRawPosition(60);
+		
+		// Then
+		
+		assertEquals(50, posFwd);
+		assertEquals(SEQUENCE.length()-60+1, posRev);
+	}
+
+	@Test
+	public void testGetTranslation() throws OutOfRangeException
+	{
+		// When
+
+		AminoAcidSequence seqFwd = fwdStrand.getTranslation(
+				new Range(10,50),
+	            true);
+		AminoAcidSequence seqRev = fwdStrand.getTranslation(
+				new Range(100,200),
+	            true);
+	
+	
+		// Then
+
+		assertEquals("gkalradggadci", seqFwd.toString());
+		assertEquals("lcvcgvfcavvsv*ggmtgckkaarrrrcsvvv", seqRev.toString());
+
+	}
+	
+	@Test
+	public void testGetSpacedTranslation() throws OutOfRangeException
+	{
+		// When
+
+		AminoAcidSequence seqFwd = fwdStrand.getSpacedTranslation(
+				new Range(10,50),
+	            true);
+		AminoAcidSequence seqRev = fwdStrand.getSpacedTranslation(
+				new Range(100,200),
+	            true);
+	
+	
+		// Then
+
+		assertEquals("g  k  a  l  r  a  d  g  g  a  d  c  i  ", seqFwd.toString());
+		assertEquals("l  c  v  c  g  v  f  c  a  v  v  s  v  *  g  g  m  t  g  c  k  k  a  a  r  r  r  r  c  s  v  v  v  ", seqRev.toString());
+
+	}
+	
+	@Test
+	public void testGetACount() throws OutOfRangeException
+	{
+		// When
+
+		int countFwd = fwdStrand.getACount();
+		int countRev = revStrand.getACount();
+	
+		// Then
+
+		assertEquals(383, countFwd);
+		assertEquals(465, countRev);
+	}
+	
+	@Test
+	public void testGetCCount() throws OutOfRangeException
+	{
+		// When
+
+		int countFwd = fwdStrand.getCCount();
+		int countRev = revStrand.getCCount();
+	
+	
+		// Then
+
+		assertEquals(506, countFwd);
+		assertEquals(575, countRev);
+	}
+	
+	@Test
+	public void testGetGCount() throws OutOfRangeException
+	{
+		// When
+
+		int countFwd = fwdStrand.getGCount();
+		int countRev = revStrand.getGCount();
+	
+	
+		// Then
+
+		assertEquals(575, countFwd);
+		assertEquals(506, countRev);
+	}
+	
+	@Test
+	public void testGetTCount() throws OutOfRangeException
+	{
+		// When
+
+		int countFwd = fwdStrand.getTCount();
+		int countRev = revStrand.getTCount();
+	
+	
+		// Then
+
+		assertEquals(465, countFwd);
+		assertEquals(383, countRev);
+	}
+
+}

From 465e43b92564acb94301d44a3e9341faa79f0148 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Fri, 25 Oct 2019 11:47:06 +0100
Subject: [PATCH 17/25] Updated release download badge version number

---
 README.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/README.md b/README.md
index d45204bad..fb2ab2ba6 100644
--- a/README.md
+++ b/README.md
@@ -14,7 +14,7 @@ The Artemis Software is a set of software tools for genome browsing and annotati
 [![status](https://img.shields.io/badge/Bioinformatics-10.1093%2Fbioinformatics%2Fbtq010-brightgreen.svg)](https://doi.org/10.1093/bioinformatics/btq010)   
 [![status](https://img.shields.io/badge/Bioinformatics-10.1093%2Fbioinformatics%2Fbtn578-brightgreen.svg)](https://doi.org/10.1093/bioinformatics/btn578)  
 
-![GitHub Releases (by Release)](https://img.shields.io/github/downloads/sanger-pathogens/artemis/v18.0.2/total)
+![GitHub Releases (by Release)](https://img.shields.io/github/downloads/sanger-pathogens/artemis/v18.1.0/total)
 
 ## Contents
   * [Introduction](#introduction)

From 716e5f2686f7f6575e385f80943cd0f9efbe62b3 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Thu, 21 Nov 2019 10:30:21 +0000
Subject: [PATCH 18/25] Added low_complexity_region and tandem_repeat feature
 keys

---
 etc/feature_keys_gff | 3 ++-
 etc/key_mapping      | 2 ++
 2 files changed, 4 insertions(+), 1 deletion(-)

diff --git a/etc/feature_keys_gff b/etc/feature_keys_gff
index afb9f5642..88c4978b8 100644
--- a/etc/feature_keys_gff
+++ b/etc/feature_keys_gff
@@ -78,4 +78,5 @@ repeat_unit     	ID Name Alias Parent Note Target Gap Derives_from Dbxref Ontolo
 #stem_loop       	ID Name Alias Parent Note Target Gap Derives_from Dbxref Ontology_term feature_id isObsolete timelastmodified allele citation evidence function gene label locus_tag map note comment operon partial standard_name 
 #terminator      	ID Name Alias Parent Note Target Gap Derives_from Dbxref Ontology_term feature_id isObsolete timelastmodified allele citation evidence gene label locus_tag map note comment operon partial standard_name 
 #transit_peptide 	ID Name Alias Parent Note Target Gap Derives_from Dbxref Ontology_term feature_id isObsolete timelastmodified allele citation codon codon_start evidence function gene label locus_tag map note comment partial product pseudo standard_name
-
+low_complexity_region	ID Name Alias Parent Note Target Gap Derives_from Dbxref Ontology_term Start_range End_range feature_id isObsolete timelastmodified note comment private score
+tandem_repeat 			ID Name Alias Parent Note Target Gap Derives_from Dbxref Ontology_term feature_id isObsolete timelastmodified score
diff --git a/etc/key_mapping b/etc/key_mapping
index 3a7f179df..75a8a9a12 100644
--- a/etc/key_mapping
+++ b/etc/key_mapping
@@ -27,3 +27,5 @@ nucleotide_match                           misc_feature    note=nucleotide_match
 uORF                                       misc_feature    note=uORF
 sORF                                       misc_feature    note=sORF
 tnaORF                                     misc_feature    note=tnaORF
+low_complexity_region                      misc_feature    note=low_complexity_region
+tandem_repeat                              repeat_region   rpt_type=tandem

From 68981aa7dad18b36e60687b32bb9e7ddb5b3f4bb Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Thu, 21 Nov 2019 10:31:26 +0000
Subject: [PATCH 19/25] Added GeneUtils entry

---
 etc/log4j.properties | 1 +
 1 file changed, 1 insertion(+)

diff --git a/etc/log4j.properties b/etc/log4j.properties
index 83ce06506..14197f0e7 100644
--- a/etc/log4j.properties
+++ b/etc/log4j.properties
@@ -46,6 +46,7 @@ log4j.logger.uk.ac.sanger.artemis.io.IndexedGFFDocumentEntry=INFO, R
 log4j.logger.uk.ac.sanger.artemis.util.FTPSeekableStream=DEBUG, R
 log4j.logger.uk.ac.sanger.artemis.plot.UserDataAlgorithm=DEBUG, R
 log4j.logger.uk.ac.sanger.artemis.ComparisonDataFactory=INFO, R
+log4j.logger.uk.ac.sanger.artemis.components.genebuilder.GeneUtils=ERROR, R
 
 #log4j.logger.com.ibatis.common.jdbc.SimpleDataSource=DEBUG, stdout, R
 #log4j.logger.com.ibatis.common.jdbc.ScriptRunner=DEBUG, stdout, R

From d782dbf864d77f44f9700d8cf2e7aad918fd3daf Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Thu, 21 Nov 2019 10:47:30 +0000
Subject: [PATCH 20/25] Test data rejig

---
 .../indexed-gff.fa => fasta/indexed-fasta.fa} |     2 +
 .../resources/data/fasta/indexed-fasta.fa.fai |     3 +
 .../resources/data/fasta/non-indexed-fasta.fa | 26471 ++++++++++++++++
 .../resources/data/gff/indexed-gff.gff.gz     |   Bin 30661 -> 31401 bytes
 .../resources/data/gff/indexed-gff.gff.gz.tbi |   Bin 539 -> 1125 bytes
 5 files changed, 26476 insertions(+)
 rename src/test/resources/data/{gff/indexed-gff.fa => fasta/indexed-fasta.fa} (99%)
 create mode 100644 src/test/resources/data/fasta/indexed-fasta.fa.fai
 create mode 100644 src/test/resources/data/fasta/non-indexed-fasta.fa

diff --git a/src/test/resources/data/gff/indexed-gff.fa b/src/test/resources/data/fasta/indexed-fasta.fa
similarity index 99%
rename from src/test/resources/data/gff/indexed-gff.fa
rename to src/test/resources/data/fasta/indexed-fasta.fa
index 04d6a8f57..4f827a59d 100644
--- a/src/test/resources/data/gff/indexed-gff.fa
+++ b/src/test/resources/data/fasta/indexed-fasta.fa
@@ -26467,3 +26467,5 @@ gggtttagggtttagggttcagggtttagggtttagggttcagggtttagggtttagggt
 ttagggtttagggtttagggttcagggtttagggtttagggtttagggttcagggtttag
 ggttcagggtttagggtttagggttcagggtttagggttcagggtttaggtgtcagggtt
 ca
+>Pf3D7_03_v3
+ttagggtttagggtttagggttcagggtttagggtttagggtttagggttcagggtttag
diff --git a/src/test/resources/data/fasta/indexed-fasta.fa.fai b/src/test/resources/data/fasta/indexed-fasta.fa.fai
new file mode 100644
index 000000000..812f8efbc
--- /dev/null
+++ b/src/test/resources/data/fasta/indexed-fasta.fa.fai
@@ -0,0 +1,3 @@
+Pf3D7_01_v3	640851	13	60	61
+Pf3D7_02_v3	947102	651558	60	61
+Pf3D7_03_v3	60	1614459	60	61
diff --git a/src/test/resources/data/fasta/non-indexed-fasta.fa b/src/test/resources/data/fasta/non-indexed-fasta.fa
new file mode 100644
index 000000000..4f827a59d
--- /dev/null
+++ b/src/test/resources/data/fasta/non-indexed-fasta.fa
@@ -0,0 +1,26471 @@
+>Pf3D7_01_v3
+tgaaccctaaaacctaaaccctaaaccctaaaccctgaaccctaaaccctgaaccctaaa
+ccctaaaccctgaaccctaaaccctaaaccctgaaccctaaaccctgaaacctaaaccct
+gaaccctaaaccctgaaccctgaaccctaaccctaaaccctaaacctaaaaccctgaacc
+ctaaaccctgaaccctgaaccctaaaccctgaaccctaaaccctaaaccctgaaccctaa
+accctgaaccctaaaccctaaaccctgaaccctgaaccctaaaacctaaaccctaaaccc
+taaaccctaaaccctgaacctaaacctaaaacctaaaacctaaaaccctgaacccttact
+tttcatttcttcttcttatcttcttacttttcattctttactcttacttacttagtctta
+cttacttactcttacttacttactcttatcttcttacttttcatttcttagtcttactta
+cttactcttacttacttactcttatcttcttacttttcattccttactcttacttactta
+ctgttatcttcttacttttcattccttactcttacttacttactcttacttacttactct
+tacttacttactcttatcttcttacttttcattccttactcttacttacttactcttact
+tacttactcttatcttcttacttttcattccttacttttcatttcttagtcttacttact
+tactcttacttacttactcttacttacttactcttactttcttcttcttatcttcttact
+gttatcttcttacttttcattccttactcttacttacttactcttatcttcttacttttc
+attccttactcttacttacttactcttatcttcttacttttcattccttactcttactta
+cttactcttatcttcttacttttcattccttactcttacttacttactcttacttactta
+ctcttacttacttactgttatcttcttacttttcattccttactcttacttacttactct
+tacttacttactcttacttacttactcttatcttcttacttttcatttgttagtcttact
+ttcttcttcttaggtccttacttttcatttcttaatcatatattcttactcatatagttc
+ttgacttaacttcttattcttacttacttactcttatattctttttatcatgttcaaggt
+cttacttcttaaatattaggtccttaatctcataattctactcttactttcttacttcta
+gaaccttattcttactttcttcatcttacatcctcagtctcaagtctttaatcttatctt
+cttactcttacttacttactcttatcttcttacttctcatttcttactcttacatacctt
+ctcttcattcttcaatcatcaattctcactcttcattccttggtcttacttctttcttct
+taatgtcatacttttatcccctattatcatctacttagtcttcatttactcttctaactt
+cttcatctatcacttttcatcatcattcatgcttacttaacttactttcatatacttact
+tctactacatcttcaccaaatccggacttagcttaacttttctctctttattcttacctt
+acttagctcttacatacttaggatctacttcttacttactactaccttacttacctctta
+tttcacttacatctaggtacttatcctactttacttctcttatcttacttctttattctc
+agattccggacttagcttaacttttttgtgtttgttcttatcttacttagctcttacata
+cttaggatctacttctttacttactttcttcttacttatgtggtaataagctactcggtt
+gggcactaaggttaggtttagctgttctttagttcttaaagggtaagaatttaaggtttg
+tattgaattattattactatttcattactattgttgctattaaggacttataatgttatg
+ttttctatccattttgttcttttatgttactaaattcattgtagattccggatttagctc
+attaactatgcttcttacttcttcttttactctttttaggtcttatgttgtatttagagt
+agtaatgttacttaattagtacttaggattcatctcatgtaggttatatgttcttataag
+gtgcacctacatgcataatatcatccaatatatctcatttagtactactatgatgactta
+tattatcattatcttacttattacttcatctactatcgtttttataccttactattacta
+attctgtctcttgaggtgcacgtatatgcacaataatgtcaattgtaactcattttttac
+tactatcatgacttatatcatatttattgtacttcttaggtcaattatggtctatatctt
+acccttttattactatttttatgttgtatatagcacttacgtgcacaattatgttcaata
+ttagtcattcattactacgattctaacttatttcatgattttcttactcttatctttatc
+tactatcattatgttaccttactattaccaaatccatgtccaatgttgcactttaatgca
+caataaggtccaacataactcatttattactactatcatcacttatatcatcattattgt
+actactaaggtcaattattgtcttcattttacctaactattactattttcatgtactata
+ttgcacttacatgcacaataacgtccaatataagtgaactacaactactattctcacata
+aggtgtcattcttctacatttaacttcatttactaacataatcttacctcactactacca
+aattcatattcttagttgcacttacatgcataattatgttcatcataactcatctactac
+tactatcatcacttatatcatcattattctacttcataagtgaactataacctgaatcgt
+accttactattactgttttcttgtccaacatagcacttatatgcacaatcatgtccaaca
+tatgtcacttactactactttgttgacttagttcatcattatctcacttataacgtcatc
+tactaacatcatcttacctaacaagtactaattccatacccaattactacttattatcgt
+catatgttgcactttaatgcataatgatgtcgaacataactcatttactactactatcct
+cacttatgtcatcattattgtacttcttagttcaattactatcatcatcttacctaatta
+ctactaaatctatcacttacgttgcacttagatgcacaataaggtccaacataactcatt
+tattactactatcctcacttatgtcatcataattctacttcttgggtcaactattatctt
+catcttacctaattactactaaatctatcacttacgttgcacttagatgcacaaacatgt
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diff --git a/src/test/resources/data/gff/indexed-gff.gff.gz b/src/test/resources/data/gff/indexed-gff.gff.gz
index 5270c10ef852a2fe2b3694cd9b335179695278dc..7eb2cb214c5c6269e21616afe11c0588073abd48 100644
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From 1d5b7c382fc7e50acc997f15311e2ae142b3e4e5 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Thu, 21 Nov 2019 10:48:21 +0000
Subject: [PATCH 21/25] Code tidy-up

---
 .../uk/ac/sanger/artemis/io/FeatureTree.java  | 25 +++++++++++--------
 1 file changed, 15 insertions(+), 10 deletions(-)

diff --git a/src/main/java/uk/ac/sanger/artemis/io/FeatureTree.java b/src/main/java/uk/ac/sanger/artemis/io/FeatureTree.java
index 8960848a5..ce3c3e1be 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/FeatureTree.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/FeatureTree.java
@@ -32,7 +32,6 @@
  *  object.
  *
  *  @author Kim Rutherford
- *  @version $Id: FeatureTree.java,v 1.3 2005-11-28 16:46:38 tjc Exp $
  **/
 
 public class FeatureTree extends TreeSet {
@@ -109,19 +108,20 @@ private void getFeaturesInRange (final TreeSet tree,
                                    final FeatureVector features_in_range,
                                    final Range range,
                                    final int max_feature_length) {
+	
+		
     // find the leftmost node in the range
-    final SortedSet tail_set =
+    final SortedSet<Feature> tail_set =
       findByBase (tree, range.getStart () - max_feature_length);
 
-    final Iterator tail_set_iterator = tail_set.iterator ();
+    final int rangeEnd = range.getEnd ();
 
     // now loop over all the features that could possibly be in the range
     // (ie. those between range.getStart () - max_feature_length and
     // range.getEnd ())
-    while (tail_set_iterator.hasNext ()) {
-      final Feature this_feature = (Feature) tail_set_iterator.next ();
+    for (Feature this_feature : tail_set) {
 
-      if (this_feature.getFirstBase () > range.getEnd ()) {
+      if (this_feature.getFirstBase () > rangeEnd) {
         return;
       }
       
@@ -145,12 +145,17 @@ public synchronized FeatureVector getFeaturesInRange (final Range range) {
     // this default size will cover many common cases
     final FeatureVector return_features = new FeatureVector();
 
-    for (int i = 0 ;
-         i < rbtree_buckets.size () && rbtree_buckets.elementAt (i) != null ;
-         ++i) {
-      getFeaturesInRange ((TreeSet) rbtree_buckets.elementAt (i),
+    final int numBuckets = rbtree_buckets.size();
+    
+    for (int i = 0; i < numBuckets; ++i) {
+    
+      TreeSet bucket = (TreeSet)rbtree_buckets.elementAt (i);
+    	
+      if (bucket != null && bucket.size() > 0) {
+    	  getFeaturesInRange (bucket,
                           return_features, range,
                           (int) Math.pow (BUCKET_MULTIPLIER, i + 1));
+      }
     }
     
     return return_features;

From 58786ab0552b9816392695cf55d24b3150587349 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Thu, 21 Nov 2019 11:06:48 +0000
Subject: [PATCH 22/25] Added NullPointer catch for missing ID

---
 .../components/genebuilder/GeneUtils.java        | 16 +++++++++++++++-
 1 file changed, 15 insertions(+), 1 deletion(-)

diff --git a/src/main/java/uk/ac/sanger/artemis/components/genebuilder/GeneUtils.java b/src/main/java/uk/ac/sanger/artemis/components/genebuilder/GeneUtils.java
index 7d8f1607d..eef44f677 100644
--- a/src/main/java/uk/ac/sanger/artemis/components/genebuilder/GeneUtils.java
+++ b/src/main/java/uk/ac/sanger/artemis/components/genebuilder/GeneUtils.java
@@ -1,7 +1,7 @@
 /* GeneUtils.java
  *
  * This file is part of Artemis
- * Copyright (C) 2007  Genome Research Limited
+ * Copyright (C) 2019  Genome Research Limited
  *
  * This program is free software; you can redistribute it and/or
  * modify it under the terms of the GNU General Public License
@@ -43,6 +43,7 @@
 import javax.swing.JPanel;
 import javax.swing.JScrollPane;
 
+import org.apache.log4j.Logger;
 import org.gmod.schema.general.DbXRef;
 import org.gmod.schema.sequence.FeatureCvTerm;
 import org.gmod.schema.sequence.FeatureDbXRef;
@@ -99,6 +100,9 @@ public class GeneUtils
   private static StringVector featuresToUpdateResidues = 
     Options.getOptions().getOptionValues("sequence_update_features");
   
+  /** Logging instance. */
+  private static Logger logger = Logger.getLogger(GeneUtils.class);
+  
   static
   {
     hideFeatures.add("polypeptide");
@@ -1453,6 +1457,16 @@ public static String getUniqueName(final Feature feature)
     {
       e.printStackTrace();
     }
+    catch(NullPointerException e)
+    {
+      StringBuilder buf = new StringBuilder(80);
+      buf.append("Feature at position ")
+         .append(feature.getFirstBase())
+         .append("-")
+         .append(feature.getLastBase())
+         .append(" has no ID");
+      logger.warn(buf.toString());
+    }
     return null;
   }
   

From 642ff907163078e08971e25628b4b5b1a2880135 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Thu, 21 Nov 2019 11:09:49 +0000
Subject: [PATCH 23/25] Changed to load database mappings up front

---
 .../sanger/artemis/io/PublicDBDocumentEntry.java  | 15 ++++++---------
 1 file changed, 6 insertions(+), 9 deletions(-)

diff --git a/src/main/java/uk/ac/sanger/artemis/io/PublicDBDocumentEntry.java b/src/main/java/uk/ac/sanger/artemis/io/PublicDBDocumentEntry.java
index 1c1756fc6..52a44da37 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/PublicDBDocumentEntry.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/PublicDBDocumentEntry.java
@@ -66,6 +66,11 @@ public class PublicDBDocumentEntry extends SimpleDocumentEntry
   
   public static boolean IGNORE_OBSOLETE_FEATURES = true;
   
+  //Load all the mappings from file
+  static {
+    initDatabaseMappings();
+  }
+  
   /**
    *  Create a new PublicDBDocumentEntry object associated with the given
    *  Document.
@@ -165,10 +170,7 @@ else if (this instanceof EmblDocumentEntry)
    * @return
    */
   private Object mapGffToNativeFeature(final Feature feature)
-  {
-    if(DATABASE_MAP_KEYS == null)
-      initDatabaseMappings();
-    
+  { 
     Key key = feature.getKey();
     QualifierVector qualifiers = feature.getQualifiers().copy();
     
@@ -571,8 +573,6 @@ private int handleTranscripts(QualifierVector qualifiers,
    */
   protected static Key mapKeys(final Key key)
   {
-    if(DATABASE_MAP_KEYS == null)
-      initDatabaseMappings();
     for(int i=0; i<DATABASE_MAP_KEYS.length; i++)
     {
       if(key.getKeyString().equals(DATABASE_MAP_KEYS[i][0]))
@@ -590,8 +590,6 @@ protected static Key mapKeys(final Key key)
    */
   private Key map(final Key key, final QualifierVector qualifiers)
   {
-    if(DATABASE_MAP_KEYS == null)
-      initDatabaseMappings();
     for(int i=0; i<DATABASE_MAP_KEYS.length; i++)
     {
       if(key.getKeyString().equals(DATABASE_MAP_KEYS[i][0]))
@@ -677,7 +675,6 @@ protected StreamSequence makeNativeSequence (final Sequence sequence)
   
   public static Object[] getDatabaseQualifiersToRemove()  
   {
-    initDatabaseMappings();
     return DATABASE_QUALIFIERS_TO_REMOVE;
   }
   

From c947be59f7723269bc57e0f3b05d6e088b228ec7 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Thu, 21 Nov 2019 11:23:32 +0000
Subject: [PATCH 24/25] Bug fixes for indexed gff handling

---
 src/main/java/uk/ac/sanger/artemis/Entry.java |   8 +-
 .../java/uk/ac/sanger/artemis/Selection.java  |   3 +-
 .../ac/sanger/artemis/SimpleEntryGroup.java   |  10 +-
 .../artemis/components/EntryFileDialog.java   |   4 +-
 .../artemis/components/FeatureList.java       |   5 +-
 .../variant/NoFeaturesException.java          |  23 --
 .../components/variant/TabixReader.java       |   5 +-
 .../artemis/io/IndexedGFFDocumentEntry.java   |  75 ++++---
 .../io/IndexedGFFDocumentEntryTest.java       | 200 +++++++++++++++++-
 9 files changed, 267 insertions(+), 66 deletions(-)
 delete mode 100644 src/main/java/uk/ac/sanger/artemis/components/variant/NoFeaturesException.java

diff --git a/src/main/java/uk/ac/sanger/artemis/Entry.java b/src/main/java/uk/ac/sanger/artemis/Entry.java
index d030da6ca..2155fa723 100644
--- a/src/main/java/uk/ac/sanger/artemis/Entry.java
+++ b/src/main/java/uk/ac/sanger/artemis/Entry.java
@@ -228,7 +228,8 @@ public void save(final File file, final int destination_type,
     final EntryInformation artemis_entry_information;
     
     if((getEMBLEntry() instanceof DatabaseDocumentEntry ||
-        getEMBLEntry() instanceof GFFDocumentEntry) &&
+        getEMBLEntry() instanceof GFFDocumentEntry || 
+        getEMBLEntry() instanceof IndexedGFFDocumentEntry) &&
        destination_type == DocumentEntryFactory.EMBL_FORMAT)
       artemis_entry_information = Options.getArtemisEntryInformation();
     else
@@ -1164,6 +1165,11 @@ private void fireAction(Vector<ChangeListener> listeners, ChangeEvent event)
   private Feature
     getFeatureOf(uk.ac.sanger.artemis.io.Feature embl_feature) 
   {
+	if (embl_feature == null)
+	{
+	  return null;
+	}
+	
     final Feature test_feature =(Feature) embl_feature.getUserData();
  
     if(test_feature == null) 
diff --git a/src/main/java/uk/ac/sanger/artemis/Selection.java b/src/main/java/uk/ac/sanger/artemis/Selection.java
index e3cc422a1..de42b98ca 100644
--- a/src/main/java/uk/ac/sanger/artemis/Selection.java
+++ b/src/main/java/uk/ac/sanger/artemis/Selection.java
@@ -239,7 +239,8 @@ public void add (final FeatureVector features) {
    *    selection.
    **/
   private boolean addWithoutEvent (final Feature feature) {
-    if (features.contains (feature)) {
+
+    if (feature != null && features.contains (feature)) {
       return false;
       // do nothing
     } else {
diff --git a/src/main/java/uk/ac/sanger/artemis/SimpleEntryGroup.java b/src/main/java/uk/ac/sanger/artemis/SimpleEntryGroup.java
index 9d1d8ee70..69f81c979 100644
--- a/src/main/java/uk/ac/sanger/artemis/SimpleEntryGroup.java
+++ b/src/main/java/uk/ac/sanger/artemis/SimpleEntryGroup.java
@@ -490,8 +490,16 @@ public FeatureVector getFeaturesInIndexRange(final int start_index,
   {
     final FeatureVector return_vector = new FeatureVector();
 
+    Feature f = null;
+    		
     for(int i = start_index; i <= end_index; ++i) 
-      return_vector.add(featureAt(i));
+    {
+      f = featureAt(i);
+      if (f != null) 
+      {
+    	  return_vector.add(featureAt(i));
+      }
+    }
 
     return return_vector;
   }
diff --git a/src/main/java/uk/ac/sanger/artemis/components/EntryFileDialog.java b/src/main/java/uk/ac/sanger/artemis/components/EntryFileDialog.java
index 47cccc69b..474e1e830 100644
--- a/src/main/java/uk/ac/sanger/artemis/components/EntryFileDialog.java
+++ b/src/main/java/uk/ac/sanger/artemis/components/EntryFileDialog.java
@@ -31,6 +31,7 @@
 import uk.ac.sanger.artemis.io.Entry;
 import uk.ac.sanger.artemis.io.DocumentEntryFactory;
 import uk.ac.sanger.artemis.io.GFFDocumentEntry;
+import uk.ac.sanger.artemis.io.IndexedGFFDocumentEntry;
 import uk.ac.sanger.artemis.io.ReadFormatException;
 import uk.ac.sanger.artemis.io.EntryInformation;
 import uk.ac.sanger.artemis.io.EntryInformationException;
@@ -470,7 +471,8 @@ else if(entry.getEMBLEntry() instanceof DatabaseDocumentEntry ||
         {
           DocumentEntryFactory.REMOVE_PRODUCT_FROM_PSEUDOGENE = removeProductForPseudo.isSelected();
           if(entry.getEMBLEntry() instanceof DatabaseDocumentEntry ||
-             entry.getEMBLEntry() instanceof GFFDocumentEntry)
+             entry.getEMBLEntry() instanceof GFFDocumentEntry ||
+             entry.getEMBLEntry() instanceof IndexedGFFDocumentEntry)
             ReadAndWriteEntry.writeDatabaseEntryToFile(entry, file, 
                 flattenGeneModel.isSelected(), 
                 ignoreObsoleteFeatures.isSelected(), false, 
diff --git a/src/main/java/uk/ac/sanger/artemis/components/FeatureList.java b/src/main/java/uk/ac/sanger/artemis/components/FeatureList.java
index c9a27abd6..0d40e32a4 100644
--- a/src/main/java/uk/ac/sanger/artemis/components/FeatureList.java
+++ b/src/main/java/uk/ac/sanger/artemis/components/FeatureList.java
@@ -450,7 +450,7 @@ private void handleCanvasMousePress(final MouseEvent event)
       final Feature clicked_feature =
         getEntryGroup().featureAt(clicked_feature_index);
 
-      if(selected_features.contains(clicked_feature)) 
+      if(clicked_feature != null && selected_features.contains(clicked_feature)) 
       {
         getSelection().remove(clicked_feature);
         getSelection().removeSegmentsOf(clicked_feature);
@@ -527,7 +527,7 @@ private void onSelectionChange()
           ++i)
       {
         final Feature this_feature = entry_group.featureAt(i);
-        if(selected_features.contains(this_feature))
+        if(this_feature != null && selected_features.contains(this_feature))
         {
           a_selected_feature_is_visible = true;
           break;
@@ -603,6 +603,7 @@ protected void paintComponent(Graphics g)
       {
         final Feature this_feature  = features_in_view.elementAt(i);
         final String feature_string = makeFeatureString(this_feature, false);
+        
         drawFeatureLine(g, this_feature, feature_string);
       }
     }
diff --git a/src/main/java/uk/ac/sanger/artemis/components/variant/NoFeaturesException.java b/src/main/java/uk/ac/sanger/artemis/components/variant/NoFeaturesException.java
deleted file mode 100644
index 3ce8c6f71..000000000
--- a/src/main/java/uk/ac/sanger/artemis/components/variant/NoFeaturesException.java
+++ /dev/null
@@ -1,23 +0,0 @@
-package uk.ac.sanger.artemis.components.variant;
-
-/**
- * An exception to be thrown when
- * a contig that has no features
- * is requested to be displayed.
- * 
- * @author kp11
- *
- */
-public class NoFeaturesException extends RuntimeException
-{
-	private static final long serialVersionUID = 1L;
-
-	/**
-	 * Constructor.
-	 * @param message - reason for exception
-	 */
-	public NoFeaturesException(String message)
-	{
-		super(message);
-	}	
-}
diff --git a/src/main/java/uk/ac/sanger/artemis/components/variant/TabixReader.java b/src/main/java/uk/ac/sanger/artemis/components/variant/TabixReader.java
index 52cc3d8d6..c770da254 100644
--- a/src/main/java/uk/ac/sanger/artemis/components/variant/TabixReader.java
+++ b/src/main/java/uk/ac/sanger/artemis/components/variant/TabixReader.java
@@ -246,7 +246,7 @@ private TIntv getIntv(final String s) {
 			if (col == mSc) {
 				intv.tid = chr2tid(s.substring(beg, end));
 			} else if (col == mBc) {
-				intv.beg = intv.end = Integer.parseInt(s.substring(beg, end));
+				intv.beg = intv.end = Integer.parseInt(s.substring(beg, end==-1?s.length():end));
 				if ((mPreset&0x10000) != 0) ++intv.end;
 				else --intv.beg;
 				if (intv.beg < 0) intv.beg = 0;
@@ -338,7 +338,7 @@ public Iterator query(final int tid, final int beg, final int end) {
 		
         if (tid == -1)
         {
-        	throw new NoFeaturesException("The current contig has no features [missing index entry].");
+        	return null;
         }
         
 		TPair64[] off, chunks;
@@ -360,6 +360,7 @@ public Iterator query(final int tid, final int beg, final int end) {
 				for (int j = 0; j < chunks.length; ++j)
 					if (less64(min_off, chunks[j].v))
 						off[n_off++] = new TPair64(chunks[j]);
+		if (n_off == 0) return null;
 		Arrays.sort(off, 0, n_off);
 		// resolve completely contained adjacent blocks
 		for (i = 1, l = 0; i < n_off; ++i) {
diff --git a/src/main/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntry.java b/src/main/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntry.java
index 3fd07673b..810d503d1 100644
--- a/src/main/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntry.java
+++ b/src/main/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntry.java
@@ -44,7 +44,6 @@
 import uk.ac.sanger.artemis.components.FeatureDisplay;
 import uk.ac.sanger.artemis.components.genebuilder.GeneUtils;
 import uk.ac.sanger.artemis.components.variant.FeatureContigPredicate;
-import uk.ac.sanger.artemis.components.variant.NoFeaturesException;
 import uk.ac.sanger.artemis.components.variant.TabixReader;
 import uk.ac.sanger.artemis.util.CacheHashMap;
 import uk.ac.sanger.artemis.util.DatabaseDocument;
@@ -63,7 +62,7 @@ public class IndexedGFFDocumentEntry implements DocumentEntry
    private String name;
    
    private String contig;
-   private boolean combinedReference = false;
+   private boolean combinedReference = true;
    
    private Document document;
    private EntryInformation entryInfo;
@@ -256,10 +255,6 @@ private void getFeaturesInRange(IndexContig c, Range range, FeatureVector featur
       tabixIterator = reader.query(r);
     }
     catch(NullPointerException npe){}
-    catch (NoFeaturesException e) 
-    {
-  	  // tabixIterator will be null
-    }
      
     if(tabixIterator == null)
       return;
@@ -357,7 +352,7 @@ private List<IndexContig> getContigsInRange(Range range)
    * Get the list of contigs in the feature display.
    * @return
    */
-  private List<IndexContig> getListOfContigs()
+  protected List<IndexContig> getListOfContigs()
   {
     List<IndexContig> contigs = new Vector<IndexContig>();
     for (String key : contigHash.keySet())
@@ -901,14 +896,7 @@ public int getFeatureCount()
       int nfeatures = 0;
       final String r = c.chr+":"+1+"-"+Integer.MAX_VALUE;
 
-      try 
-      {
-    	  tabixIterator = reader.query(r);
-      } 
-      catch (NoFeaturesException e) 
-      {
-    	  // tabixIterator will be null
-      }
+      tabixIterator = reader.query(r);
       
       if(tabixIterator == null)
         continue;
@@ -959,7 +947,7 @@ public Feature getFeatureAtIndex(int idx)
     for(IndexContig c: contigs)
     {
       int nfeatures = c.nfeatures;
-      if(idx > cnt+nfeatures)
+      if(nfeatures == 0 || idx > cnt+nfeatures)
       {
         cnt+=nfeatures;
         continue;
@@ -967,8 +955,10 @@ public Feature getFeatureAtIndex(int idx)
       String r = c.chr+":"+start+"-"+Integer.MAX_VALUE;
 
       TabixReader.Iterator tabixIterator = reader.query(r);
+      
       if(tabixIterator == null)
         return null;
+      
       try
       {
         String ln;
@@ -1035,9 +1025,12 @@ public int indexOf(Feature feature)
       }
       
       String r = c.chr+":"+1+"-"+Integer.MAX_VALUE;
+      
       TabixReader.Iterator tabixIterator = reader.query(r);
+      
       if(tabixIterator == null)
         continue;
+      
       try
       {
         String ln;
@@ -1329,6 +1322,16 @@ private int getOffsetEnd()
     {
       return end+offset;
     }
+    
+    private int getStart()
+    {
+      return start;
+    }
+    
+    private int getEnd()
+    {
+      return end;
+    }
   }
 
   class ContigCompare implements Comparator<IndexContig>
@@ -1343,6 +1346,9 @@ else if(c2.offset > c1.offset)
     }
   }
   
+  /**
+   * Iterates through all features of an Indexed GFF file.
+   */
   class IndexGFFFeatureEnumeration implements FeatureEnumeration
   { 
     private FeatureVector features;
@@ -1366,19 +1372,21 @@ public boolean hasMoreFeatures()
           }
           catch (OutOfRangeException e){}
         }
-        getFeaturesInContig();
+        getFeaturesInFirstContig();
       }
 
       if(idx < features.size())
         return true;
       else
       {
-        idx = 0;
-        contigIdx++;
-        if(contigIdx < contigs.size())
-        {
+    	idx = 0;
+    	  
+    	// we have to be careful about sequences with no features here...
+    	while (++contigIdx < contigs.size())
+        {   
           getFeaturesInContig();
-          if(idx < features.size())
+            
+          if(features.size() > 0)
             return true;
         }
       }
@@ -1391,13 +1399,32 @@ public Feature nextFeature() throws NoSuchElementException
       return features.elementAt(idx-1);
     }
     
+    /**
+     * Populate the features collection with all features in the 
+     * contig given by the start and end offsets.
+     */
     private void getFeaturesInContig()
     {
       try
       {
-        features = getFeaturesInRange(
+          features = getFeaturesInRange(
             new Range(contigs.get(contigIdx).getOffsetStart(), 
-                      contigs.get(contigIdx).getOffsetEnd()));
+            		  contigs.get(contigIdx).getOffsetEnd()));
+      }
+      catch (OutOfRangeException e){}
+    }
+    
+    /**
+     * Populate the features collection with all features in the 
+     * first contig.
+     */
+    private void getFeaturesInFirstContig()
+    {
+      try
+      {
+    	features = getFeaturesInRange(
+    	    new Range(contigs.get(0).getStart(), 
+    	              contigs.get(0).getEnd()));
       }
       catch (OutOfRangeException e){}
     }
diff --git a/src/test/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntryTest.java b/src/test/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntryTest.java
index 57be1defa..1c2aa2f8b 100644
--- a/src/test/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntryTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/io/IndexedGFFDocumentEntryTest.java
@@ -20,17 +20,30 @@
  */
 package uk.ac.sanger.artemis.io;
 
-import static org.junit.Assert.fail;
+import static org.junit.Assert.assertEquals;
+import static org.junit.Assert.assertNull;
+import static org.mockito.Mockito.when;
 
+import java.io.File;
 import java.io.IOException;
+import java.net.URL;
+import java.util.HashMap;
+
+import org.junit.Before;
 import org.junit.Test;
+import org.mockito.Mock;
+import org.mockito.MockitoAnnotations;
 
-import uk.ac.sanger.artemis.components.variant.NoFeaturesException;
+import htsjdk.samtools.util.BlockCompressedInputStream;
+import uk.ac.sanger.artemis.EntryGroup;
+import uk.ac.sanger.artemis.SimpleEntryGroup;
 import uk.ac.sanger.artemis.components.variant.TabixReader;
+import uk.ac.sanger.artemis.sequence.Bases;
+import uk.ac.sanger.artemis.util.FileDocument;
+import uk.ac.sanger.artemis.util.OutOfRangeException;
 
 /**
  * Unit test for the IndexedGFFDocumentEntry class.
- * It currently only covers new functionality/changes.
  * 
  * @author kp11
  *
@@ -38,7 +51,83 @@
 public class IndexedGFFDocumentEntryTest
 {
 
-	public static final String TEST_INDEXED_GFF_FILE = "/data/gff/indexed-gff.gff.gz";
+	private final String INDEXED_GFF_FILE = "/data/gff/indexed-gff.gff.gz";
+	private final String INDEXED_GFF_FASTA_FILE = "/data/fasta/non-indexed-fasta.fa";
+	
+	private static final HashMap<Integer, String[]> ENUMERATION_FEATURES = new HashMap<Integer, String[]>();
+	static {
+		// Check both contigs
+		ENUMERATION_FEATURES.put(1, new String[]{ "Pfalciparum_REP_20", "1", "360" });
+		ENUMERATION_FEATURES.put(2, new String[]{ "Pfalciparum_REP_15", "361", "1418" });
+		ENUMERATION_FEATURES.put(3, new String[]{ "Pfalciparum_REP_35", "2160", "3858" });
+		ENUMERATION_FEATURES.put(4, new String[]{ "Pfalciparum_REP_5", "8856", "9021" });
+		ENUMERATION_FEATURES.put(5, new String[]{ "Pfalciparum_REP_25", "9313", "9529" });
+		ENUMERATION_FEATURES.put(6, new String[]{ "Pfalciparum_REP_55", "11315", "27336" });
+		ENUMERATION_FEATURES.put(7, new String[]{ "PF3D7_0100100", "29510", "37126" });
+		ENUMERATION_FEATURES.put(8, new String[]{ "PF3D7_0100100.1", "29510", "37126" });
+		ENUMERATION_FEATURES.put(9, new String[]{ "PF3D7_0100100.1:pep", "29510", "37126" });
+		ENUMERATION_FEATURES.put(10, new String[]{ "PF3D7_0100200", "38982", "40207" });
+		ENUMERATION_FEATURES.put(11, new String[]{ "PF3D7_0100200.1", "38982", "40207" });
+		ENUMERATION_FEATURES.put(12, new String[]{ "PF3D7_0100200.1:pep", "38982", "40207" });
+		ENUMERATION_FEATURES.put(13, new String[]{ "PF3D7_0100300", "42367", "46507" });
+		
+		ENUMERATION_FEATURES.put(629, new String[]{ "PF3D7_0109600.1:exon{3,2,1}", "377874", "378599" });
+		ENUMERATION_FEATURES.put(630, new String[]{ "Pf3D7_02__new:1..1142", "640852", "641993" });
+		
+		ENUMERATION_FEATURES.put(1579, new String[]{ "PF3D7_0200600.1:exon{2,1}", "686137", "687409" });
+		ENUMERATION_FEATURES.put(1580, new String[]{ "PF3D7_0214200.1:exon{2,1}", "1216006", "1216905" });
+		ENUMERATION_FEATURES.put(1581, new String[]{ "PF3D7_0216500.1:exon{1,2}", "1319338", "1324554" });
+		ENUMERATION_FEATURES.put(1582, new String[]{ "PF3D7_0218000.1:exon{1}", "1379711", "1380703" });
+	};
+	
+	private final static String [][] EXPECTED_CONTIG1_FEATURES = 
+		{
+				{ "Pfalciparum_REP_15", "361", "1418" },
+				{ "Pfalciparum_REP_35", "2160", "3858" },
+				{ "Pfalciparum_REP_5", "8856", "9021" },
+				{ "Pfalciparum_REP_25", "9313", "9529" },
+				{ "Pfalciparum_REP_55", "11315", "27336" },
+				{ "PF3D7_0100100", "29510", "37126" },
+				{ "PF3D7_0100100.1", "29510", "37126" },
+				{ "PF3D7_0100100.1:pep", "29510", "37126" },
+				{ "PF3D7_0100100.1:exon{1,2}", "29510", "37126" }
+		};
+	
+	private final static String [][] EXPECTED_CONTIG2_FEATURES = 
+		{
+				{ "Pf3D7_02__new:1..1142", "640852", "641993" },
+				{ "PF3D7_0200100", "666083", "672019" },
+				{ "PF3D7_0200100.1", "666083", "672019" },
+				{ "PF3D7_0200100.1:pep", "666083", "672019" },
+				{ "PF3D7_0200100.1:exon{1,2}", "666083", "672019" }
+		};
+	
+	@Mock
+	private FileDocument doc;
+	
+	@Before
+	public void setUp() throws Exception {
+
+		MockitoAnnotations.initMocks(this);
+	}
+	
+	/**
+	 * Load a data file.
+	 * @param file String
+	 * @return Entry
+	 */
+	protected Entry loadFile(String file)
+	{
+		return Utils.getEntry(file);
+	}
+	
+	protected File getFile(String filePath)
+	{
+		URL filePathUrl = IndexedGFFDocumentEntryTest.class.getResource(filePath);
+		File file = new File(filePathUrl.getFile());
+		
+		return file;
+	}
 	
 	/**
 	 * Test the query method.
@@ -48,16 +137,105 @@ public class IndexedGFFDocumentEntryTest
 	public void testQuery() throws IOException 
 	{	
 		TabixReader reader = new TabixReader(
-				IndexedGFFDocumentEntryTest.class.getResource(TEST_INDEXED_GFF_FILE).getFile());
+				IndexedGFFDocumentEntryTest.class.getResource(INDEXED_GFF_FILE).getFile());
+
+		TabixReader.Iterator tabixIterator = reader.query(-1, 1, 19);
+		assertNull(tabixIterator);
+	}
+	
+	/**
+	 * General testing of feature enumeration code.
+	 * @throws IOException
+	 * @throws OutOfRangeException
+	 * @throws InvalidRelationException
+	 */
+	@Test
+	public void testFeatureEnumeration() throws IOException, OutOfRangeException, InvalidRelationException
+	{
+		int numFeatures = 0;
+		
+		// Given 
+		
+		// We don't want GUI pop-ups displayed during unit tests...
+		DocumentEntryFactory.setDisplayIndexingQuestionForGffs(false);
+		
+		final File file = getFile(INDEXED_GFF_FILE);
+		final BlockCompressedInputStream stream = new BlockCompressedInputStream(file);
+
+		// When
+		
+		when( doc.getFile() ).thenReturn (file);
+		when( doc.getInputStream() ).thenReturn (stream);
+		
+		final IndexedGFFDocumentEntry indexedGffEntry = new IndexedGFFDocumentEntry(doc);
+		final Entry fastaEntry = loadFile(INDEXED_GFF_FASTA_FILE);
+		
+		final Bases bases = new Bases(fastaEntry.getSequence());
+		final EntryGroup group = new SimpleEntryGroup(bases);
+		final uk.ac.sanger.artemis.Entry artFastaEntry = new uk.ac.sanger.artemis.Entry(bases, fastaEntry);
+		final uk.ac.sanger.artemis.Entry artGffEntry = new uk.ac.sanger.artemis.Entry(bases, indexedGffEntry);
+		group.add(artFastaEntry);
+		group.add(artGffEntry);
+		
+		// Then
 		
-		try 
+		final uk.ac.sanger.artemis.io.FeatureEnumeration feature_enumerator = indexedGffEntry.features();
+		while (feature_enumerator.hasMoreFeatures())
 		{
-			reader.query(-1, 1, 19);
-			fail("Expected a NoFeaturesException to be thrown");
-		} 
-		catch (NoFeaturesException e)
+			Feature feature = feature_enumerator.nextFeature();
+			
+			++numFeatures;
+			
+			/*System.out.println(
+					numFeatures + ": " + 
+					feature.getQualifierByName("ID").getValues().elementAt(0) + " " + 
+					feature.getFirstBase() + " " + 
+					feature.getLastBase());*/
+			
+			// Check a sample of values...
+			
+			if (ENUMERATION_FEATURES.containsKey(numFeatures)) 
+			{
+				assertEquals("Feature index " + numFeatures + " ID", ENUMERATION_FEATURES.get(numFeatures)[0], feature.getQualifierByName("ID").getValues().elementAt(0));
+				assertEquals("Feature index " + numFeatures + " start", ENUMERATION_FEATURES.get(numFeatures)[1], Integer.toString(feature.getFirstBase()));
+				assertEquals("Feature index " + numFeatures + " end", ENUMERATION_FEATURES.get(numFeatures)[2], Integer.toString(feature.getLastBase()));
+			}
+		}
+
+		assertEquals(1582, numFeatures);
+		
+		// Test getListOfContigs while we're at it - 3 contigs (one with no features)
+		assertEquals(3, indexedGffEntry.getListOfContigs().size());
+		
+		//Contig 1
+		FeatureVector list = indexedGffEntry.getFeaturesInRange(new Range(361, 34762));
+		assertEquals(9, list.size());
+		
+		int idx = 0;
+		for (Feature feature : list)
 		{
-			// expected
+			//System.out.println("Contig1: " + feature.getQualifierByName("ID").getValues().elementAt(0) + " " + feature.getFirstBase() + " " + feature.getLastBase());
+			assertEquals(EXPECTED_CONTIG1_FEATURES[idx][0], feature.getQualifierByName("ID").getValues().elementAt(0));
+			assertEquals(EXPECTED_CONTIG1_FEATURES[idx][1], Integer.toString(feature.getFirstBase()));
+			assertEquals(EXPECTED_CONTIG1_FEATURES[idx][2], Integer.toString(feature.getLastBase()));
+			
+			++idx;
 		}
+		
+		// Contig 2 - start features
+		list = indexedGffEntry.getFeaturesInRange(new Range(640852, 672020));
+		assertEquals(5, list.size());
+		
+		idx = 0;
+		for (Feature feature : list)
+		{
+			//System.out.println("Contig2: " + feature.getQualifierByName("ID").getValues().elementAt(0) + " " + feature.getFirstBase() + " " + feature.getLastBase());
+			assertEquals(EXPECTED_CONTIG2_FEATURES[idx][0], feature.getQualifierByName("ID").getValues().elementAt(0));
+			assertEquals(EXPECTED_CONTIG2_FEATURES[idx][1], Integer.toString(feature.getFirstBase()));
+			assertEquals(EXPECTED_CONTIG2_FEATURES[idx][2], Integer.toString(feature.getLastBase()));
+			
+			++idx;
+		}
+		
 	}
 }

From 993d8c9480afa889fb7c1a1a7b327ee65f61a2ad Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=E2=80=9Ckpepper=E2=80=9D?= <kp11@sanger.ac.uk>
Date: Thu, 21 Nov 2019 11:25:24 +0000
Subject: [PATCH 25/25] Updated in line with test data changes

---
 .../artemis/io/SimpleDocumentEntryTest.java   | 39 ++++++++-----------
 1 file changed, 16 insertions(+), 23 deletions(-)

diff --git a/src/test/java/uk/ac/sanger/artemis/io/SimpleDocumentEntryTest.java b/src/test/java/uk/ac/sanger/artemis/io/SimpleDocumentEntryTest.java
index 054e7d9cb..8fc2ceb54 100644
--- a/src/test/java/uk/ac/sanger/artemis/io/SimpleDocumentEntryTest.java
+++ b/src/test/java/uk/ac/sanger/artemis/io/SimpleDocumentEntryTest.java
@@ -50,10 +50,6 @@
  * JUnit test suite for the SimpleDocumentEntry class
  * and underlying functionality (more of an integration test really!).
  * 
- * This test module carries out multiple tests in each test method
- * - this is done for efficiency reasons to avoid having to 
- *   repeatedly load test files.
- * 
  * @author kp11
  *
  */
@@ -64,7 +60,7 @@ public class SimpleDocumentEntryTest
 	
 	// Indexed
 	private final String INDEXED_GFF_FILE = "/data/gff/indexed-gff.gff.gz";
-	private final String INDEXED_GFF_FASTA_FILE = "/data/gff/indexed-gff.fa";
+	private final String NON_INDEXED_FASTA_FILE = "/data/fasta/non-indexed-fasta.fa";
 	private final int NUM_INDEXED_GFF_FEATURES = 2238;
 	
 	// Non-indexed
@@ -72,11 +68,12 @@ public class SimpleDocumentEntryTest
 	
 	private final String GFF_OUTPUT_FILE = "out.gff3";
 	private final int NUM_GFF_FEATURES = 1584;
-	private final int GFF_SEQ_LEN = 1587953;
+	private final int GFF_SEQ_LEN = 1588013;
 	private final String GFF_FEATURE_ID_KEY = "ID";
 	
 	private final String GFF_SEQ_START = "tgaaccctaaaacctaaaccctaaaccctaaaccctgaaccctaaaccctgaaccctaaaccctaaaccctgaac";
-	private final String GFF_SEQ_END = "tagggttcagggtttaggtgtcagggttca";
+	private final String GFF_SEQ_END = "ttagggtttagggtttagggttcagggtttagggtttagggtttagggttcagggtttag";
+	private final String NONINDEXED_GFF_SEQ_END = "tagggttcagggtttaggtgtcagggttca";
 			
 	private final String GFF_HDRS = 
 			"##gff-version 3\n" + 
@@ -631,14 +628,14 @@ public void testNonIndexedGFF() throws Exception
 		String seqString = String.valueOf( sequence.getCharSubSequence(1, sequence.length()) );
 		
 		assertTrue(sequence instanceof FastaStreamSequence);
-		assertEquals( GFF_SEQ_LEN, seqString.length() );
+		assertEquals( 1587953, seqString.length() );
 		assertEquals( 639816, sequence.getACount() );
 		assertEquals( 159003, sequence.getCCount() );
 		assertEquals( 159425, sequence.getGCount() );
 		assertEquals( 629709, sequence.getTCount() );
 		
 		assertTrue(seqString.indexOf(GFF_SEQ_START) == 0);
-		assertTrue(seqString.lastIndexOf(GFF_SEQ_END) == (seqString.length()-GFF_SEQ_END.length()));
+		assertTrue(seqString.lastIndexOf(NONINDEXED_GFF_SEQ_END) == (seqString.length()-NONINDEXED_GFF_SEQ_END.length()));
 
 		//printFeatures("testNonIndexedGFF", entry);
 		
@@ -781,10 +778,10 @@ public void testIndexedGFF() throws Exception
 		when( saveToDoc.getOutputStream() ).thenReturn (outStream);
 		
 		final Entry entry = loadFile(INDEXED_GFF_FILE);
-		final Entry fastaEntry = loadFile(INDEXED_GFF_FASTA_FILE);
+		final Entry fastaEntry = loadFile(NON_INDEXED_FASTA_FILE);
 		
 		final Bases bases = new Bases(fastaEntry.getSequence());
-		final EntryGroup group = new SimpleEntryGroup(bases);
+		final EntryGroup group = new SimpleEntryGroup();
 		final uk.ac.sanger.artemis.Entry artFastaEntry = new uk.ac.sanger.artemis.Entry(bases, fastaEntry);
 		final uk.ac.sanger.artemis.Entry artGffEntry = new uk.ac.sanger.artemis.Entry(bases, entry);
 		group.add(artFastaEntry);
@@ -824,26 +821,26 @@ public void testIndexedGFF() throws Exception
 		nextFeature = artGffEntry.getFeature(idx);
 		assertEquals( "gene", nextFeature.getKey().getKeyString() );
 		assertEquals( "PF3D7_0100200", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
-		assertEquals( 1547747, nextFeature.getFirstBase() );
-		assertEquals( 1548972, nextFeature.getLastBase() );
+		assertEquals( 1547807, nextFeature.getFirstBase() );
+		assertEquals( 1549032, nextFeature.getLastBase() );
 		
 		nextFeature = artGffEntry.getFeature(++idx);
 		assertEquals( "mRNA", nextFeature.getKey().getKeyString() );
 		assertEquals( "PF3D7_0100200.1", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
-		assertEquals( 1547747, nextFeature.getFirstBase() );
-		assertEquals( 1548972, nextFeature.getLastBase() );
+		assertEquals( 1547807, nextFeature.getFirstBase() );
+		assertEquals( 1549032, nextFeature.getLastBase() );
 		
 		nextFeature = artGffEntry.getFeature(++idx);
 		assertEquals( "polypeptide", nextFeature.getKey().getKeyString() );
 		assertEquals( "PF3D7_0100200.1:pep", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
-		assertEquals( 1547747, nextFeature.getFirstBase() );
-		assertEquals( 1548972, nextFeature.getLastBase() );
+		assertEquals( 1547807, nextFeature.getFirstBase() );
+		assertEquals( 1549032, nextFeature.getLastBase() );
 		
 		nextFeature = artGffEntry.getFeature(++idx);
 		assertEquals( "CDS", nextFeature.getKey().getKeyString() );
 		assertEquals( "PF3D7_0100200.1:exon:2", nextFeature.getQualifierByName(GFF_FEATURE_ID_KEY).getValues().elementAt(0) );
-		assertEquals( 1547747, nextFeature.getFirstBase() );
-		assertEquals( 1547800, nextFeature.getLastBase() );
+		assertEquals( 1547807, nextFeature.getFirstBase() );
+		assertEquals( 1547860, nextFeature.getLastBase() );
 		
 		idx = 22;
 		nextFeature = artGffEntry.getFeature(idx);
@@ -938,10 +935,6 @@ public void testNonIndexedFastaFile() throws Exception
 		
 		log("testNonIndexedFastaFile", "Writing to file: " + savefile.getAbsolutePath());
 		
-		// We don't want GUI pop-ups displayed during unit tests...
-		DocumentEntryFactory.setDisplayIndexingQuestionForGffs(false);
-		
-		
 		// When
 		
 		when( saveToDoc.getFile() ).thenReturn (savefile);