gnuplot: error while loading shared libraries

[dppss90008]

Hello, there

I tried my-brain-seq for analyzing my small RNA sequencing dat. I ran your tool using docker through the command from your introduction.

Step 1_fastqc; 2_cutadapt; 3_bowtie are all processed successfully.
However, step 4_bam_stats raised an error stating that there is no libQt5Core.so.5 in your Docker images.

Here is the error logs from bam-stats

-- Starting log on Tue Aug 13 06:08:58 GMT 2024 --

-- Iteration: trimmed_SAMPLE_N4_S999_R1_001.fastq.bam--
gnuplot: error while loading shared libraries: libQt5Core.so.5: cannot open shared object file: No such file or directory
The command exited with non-zero status 32512:
gnuplot /data/hsieh/project/test//output/4_bam_stats/trimmed_SAMPLE_N4_S999_R1_001/trimmed_ACO_N4_S999_R1_001.fastq.bam_PLOT-acgt-cycles.gp

at /usr/bin/plot-bamstats line 51.
main::error("The command exited with non-zero status 32512:\x{a}\x{9}gnuplot /data"...) called at /usr/bin/plot-bamstats line 317
main::plot("/data/hsieh/project/test//output/4_bam_stats/trimmed_SAMPLE_N4_S"...) called at /usr/bin/plot-bamstats line 855
main::plot_acgt_cycles(HASH(0x55e7b8d83ee8)) called at /usr/bin/plot-bamstats line 34

By the way, I have provided the Docker images that my-brain-seq pulled. This might help you debug the issue.

[hlfernandez]

Hi @dppss90008, thank you for your interest in myBrain-Seq. Could you please tell which versions of operating system and Docker are you currently using? We need to reproduce the problem by ourselves to figure out a solution.

[dppss90008]

Hi @hlfernandez

This is the OS and Docker we used.
CentOS Linux release 7.9.2009 (Core)
Docker version 26.1.4, build 5650f9b

Thanks