1. Prerequisites

Input data & preprocessing tools

Ninetails requires the following input data to operate:

• multifast5 files basecalled by Guppy - for the signal data extraction
• sequencing_summary.txt file - for file ID extraction
• an output of Nanopolish polya function (tsv file) - to obtain the tail segmentation data*

Therefore, please make sure that the third-party software necessary for the steps preceding the use of Ninetails is installed (Nanopolish, Guppy) and/or that you have all the required input files.

For neural network component of Ninetails (data classification), Python is also required.

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*since version 1.0.2, Ninetails is also compatible with tailfindR. However, we still strongly recommend to use it with nanopolish.