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Changed MPN-RFU for fluorMPN to match manuscript.
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karlapaolafranco authored Sep 13, 2023
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Expand Up @@ -75,11 +75,11 @@ MPN values were calculated with the Python package ["MicroMPN"](https://github.c

**4. Folder**: multiple_comparisons

- To assess if a community’s suppressive activity was independent of the method (MPN-RFU microplate assay vs spotting on agar) being used, we compared the mean of every community to the mean of the control (rfp-Rs5).
- To assess if a community’s suppressive activity was independent of the method (fluorMPN microplate assay vs spotting on agar) being used, we compared the mean of every community to the mean of the control (rfp-Rs5).

- 4.1. *Subfolder*: R_analysis

- This folder contains two files, an R script ("backup_oneway_dataMPN_micrompn_trim_norarity_community_R.txt") and an input ("data_micrompn_trim_norarity_community_R.csv") file. Differences between group means for the MPN-RFU microplate assay were compared by a one-way ANOVA, followed by the post-hoc test for multiple comparisons Tukey’s Honest Significant Differences. However, for spotting on agar, since the assumption of normality was violated, we ran the non-parametric test Kruskal-Wallis, followed by Dunn’s test for multiple comparisons. The input file contains both MPN and MPN-adjusted data. Users can run the analysis with MPN-adjusted values by simply subsetting for the correct columns in the input file.
- This folder contains two files, an R script ("backup_oneway_dataMPN_micrompn_trim_norarity_community_R.txt") and an input ("data_micrompn_trim_norarity_community_R.csv") file. Differences between group means for the fluorMPN microplate assay were compared by a one-way ANOVA, followed by the post-hoc test for multiple comparisons Tukey’s Honest Significant Differences. However, for spotting on agar, since the assumption of normality was violated, we ran the non-parametric test Kruskal-Wallis, followed by Dunn’s test for multiple comparisons. The input file contains both MPN and MPN-adjusted data. Users can run the analysis with MPN-adjusted values by simply subsetting for the correct columns in the input file.

- 4.2. *Subfolder*: Excel_spreadsheet

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