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LilyAnderssonLee authored Nov 27, 2023
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# ![nf-core/metaval](docs/images/nf-core-metaval_logo_light.png#gh-light-mode-only) ![nf-core/metaval](docs/images/nf-core-metaval_logo_dark.png#gh-dark-mode-only)
# metaval

[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/)
[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
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## Introduction

**nf-core/metaval** is a bioinformatics pipeline that verifies the organisms predicted by the nf-core/taxprofiler pipeline using metagenomic data, including both Illumina short-gun sequencing and Nanopore sequencing data.
**metaval** is a bioinformatics pipeline that verifies the organisms predicted by the nf-core/taxprofiler pipeline using metagenomic data, including both Illumina short-gun sequencing and Nanopore sequencing data.

At moment, meta-val only checks the classification results from three classifiers `Kraken2`, `Centrifuge` and `diamond`
At moment, metaval only checks the classification results from three classifiers `Kraken2`, `Centrifuge` and `diamond`

## Pipeline summary

1. Extract classified reads for organisms of interest, such as all identified viruses or a predefined list of organisms.

2. Use `BLAST` to identify the closet reference genome for the extracted reads.
2. Use `BLAST` to identify the closet reference genome for the extracted reads (Downsample if there are more than 200 reads)

3. Map the extracted reads to reference genomes using `Bowtie2` for Illumina reads and `minimap2` for Nanopore reads.

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