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Update README
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jaleezyy authored Feb 16, 2023
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Expand Up @@ -87,17 +87,22 @@ To display the help screen:
python signal.py -h
Output:
usage: signal.py [-h] [-c CONFIGFILE] [-d DIRECTORY] [--cores CORES] [--config-only] [--remove-freebayes] [--add-breseq] [-neg NEG_PREFIX] [--dependencies]
usage: signal.py [-h] [-c CONFIGFILE] [-d DIRECTORY] [--cores CORES] [--config-only] [--remove-freebayes] [--add-breseq]
[-neg NEG_PREFIX] [--dependencies] [-ri] [--unlock] [-F] [-n] [--verbose] [-v]
[all ...] [postprocess ...] [ncov_tools ...]
SARS-CoV-2 Illumina GeNome Assembly Line (SIGNAL) aims to take Illumina short-read sequences and perform consensus assembly + variant calling for ongoing surveillance and research efforts towards
the emergent coronavirus: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2).
SARS-CoV-2 Illumina GeNome Assembly Line (SIGNAL) aims to take Illumina short-read sequences and perform consensus assembly +
variant calling for ongoing surveillance and research efforts towards the emergent coronavirus: Severe Acute Respiratory Syndrome
Coronavirus 2 (SARS-CoV-2).
positional arguments:
all Run SIGNAL with all associated assembly rules. Does not include postprocessing '--configfile' or '--directory' required. The latter will automatically generate a
configuration file and sample table. If both provided, then '--configfile' will take priority
postprocess Run SIGNAL postprocessing on completed SIGNAL run. '--configfile' is required but will be generated if '--directory' is provided
ncov_tools Generate configuration file and filesystem setup required and then execute ncov-tools quality control assessment. '--configfile' is required but will be generated if '--
all Run SIGNAL with all associated assembly rules. Does not include postprocessing '--configfile' or '--
directory' required. The latter will automatically generate a configuration file and sample table. If
both provided, then '--configfile' will take priority
postprocess Run SIGNAL postprocessing on completed SIGNAL run. '--configfile' is required but will be generated if '
--directory' is provided
ncov_tools Generate configuration file and filesystem setup required and then execute ncov-tools quality control
assessment. Requires 'ncov-tools' submodule! '--configfile' is required but will be generated if '--
directory' is provided
optional arguments:
Expand All @@ -108,12 +113,23 @@ optional arguments:
Path to directory containing reads. Will be used to generate sample table and configuration file
--cores CORES Number of cores. Default = 1
--config-only Generate sample table and configuration file (i.e., config.yaml) and exit. '--directory' required
--remove-freebayes Configuration file generator parameter. Set flag to DISABLE freebayes variant calling (improves overall speed)
--add-breseq Configuration file generator parameter. Set flag to ENABLE optional breseq step (will take more time for analysis to complete)
--remove-freebayes Configuration file generator parameter. Set flag to DISABLE freebayes variant calling (improves overall
speed)
--add-breseq Configuration file generator parameter. Set flag to ENABLE optional breseq step (will take more time for
analysis to complete)
-neg NEG_PREFIX, --neg-prefix NEG_PREFIX
Configuration file generator parameter. Comma-separated list of negative sontrol sample name(s) or prefix(es). For example, 'Blank' will cover Blank1, Blank2, etc. Recommend
if running ncov-tools. Will be left empty, if not provided
--dependencies Download data dependencies (under a created 'data' directory) required for SIGNAL analysis and exit. Note: Will override other flags! (~10 GB storage required)
Configuration file generator parameter. Comma-separated list of negative sontrol sample name(s) or
prefix(es). For example, 'Blank' will cover Blank1, Blank2, etc. Recommended if running ncov-tools. Will
be left empty, if not provided
--dependencies Download data dependencies (under a created 'data' directory) required for SIGNAL analysis and exit.
Note: Will override other flags! (~10 GB storage required)
-ri, --rerun-incomplete
Snakemake parameter. Re-run any incomplete samples from a previously failed run
--unlock Snakemake parameter. Remove a lock on the working directory after a failed run
-F, --forceall Snakemake parameter. Force the re-run of all rules regardless of prior output
-n, --dry-run Snakemake parameter. Do not execute anything and only display what would be done
--verbose Snakemake parameter. Display snakemake debugging output
-v, --version Display version number
```

## Summary:
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