git clone https://github.com/jameslz/denoise-kit
wget https://drive5.com/downloads/usearch11.0.667_i86linux32.gz
gunzip usearch11.0.667_i86linux32.gz
mv usearch11.0.667_i86linux32 usearch
chmod -R 775 usearch
mv usearch denoise-kit/binaries
export PATH=$PWD/denoise-kit:$PATH
cp $PWD/denoise-kit/example/* ./
: modifiy template files: metdata.txt and mapping_file.txt
For raw reads file format, support:
ZB1_1.fastq.gz ZB1_2.fastq.gz
ZB1_1.fastq ZB1_2.fastq
run pipeline:
denoise-kit metdata.txt pipeline