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Mohammad Reza Bakhtiarizadeh edited this page Jul 19, 2017
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Welcome to the OOgenesis_Pred wiki!
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| Oogenesis Protein Predictor by: |
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| Mohammad Reza Bakhtiarizadeh |
| Maryam Rahimi |
| Abdollah MohaMmadi Sangcheshmeh |
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| University of Tehran$ |
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| Contact: mrbakhtiari@ut.ac.ir |
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| Usage: /path/to/OOgenesis_Pred /path/to/sequences.fasta |
|______________________________________________________________________|
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Introduction
OOgenesis_pred is a Support Vector Machine-based classifier to predict the oogenesis related proteins based on 1920 meaningful protein sequence features. It takes protein FASTA sequences as input, and generate output about the potential of a protein to be involved in oogenesis. OOgenesis_pred depends on two programs (libsvm and protr package in R) and can be run on Linux.
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Pre-requisite
It just need R software to be installed in your system.
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Install dependencies
Drag install.sh file to terminal for automatic installing all of the dependencies. This will build and install the libsvm software and protr package. $ gzip -dc OOgenesis_Pred.tar.gz | tar x $ cd OOgenesis_Pred $ chmod 777 /full/path/to/install.sh $ /full/path/to/install.sh
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Run OOgenesis_Pred
$ /full/path/to/OOgenesis_Pred /full/path/to/Candidates.fa
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Output
The output files will be stored in OOgenesis_Pred_resultss.txt. An example of OOgenesis_Pred output is presented here. The score represents a protein's probability of belonging to the oogenesis. OOgenesis_Pred applied a probability score >0.5 to designate putative oogenesis related protein.
############################ # OOgenesis_Pred Results # # # # 21/04/2016 17:30:03 # ############################ Total number of processed sequences: 3 Total number of predicted sequences as Ogenesis candidate: 3 __________________________________________________________________________________ Protein_ID Prediction Score __________________________________________________________________________________ sp|Q920Q6|MSI2H_MOUSE Protein is Ogenesis candidate 0.595419 __________________________________________________________________________________ sp|Q9GSX9|RIC8_CAEEL Protein is Ogenesis candidate 0.904385 __________________________________________________________________________________ sp|P42345|MTOR_HUMAN Protein is Ogenesis candidate 0.682906 __________________________________________________________________________________