4. Test installation

Begin with test dataset

a. Open test data file

gunzip dbOTUcaller_testdata.tar.gz

tar -xvomf dbOTUcaller_testdata.tar

Begin by executing the following command in example_data1:

python /home/spacocha/lib/dbOTUcaller/dbOTUcaller.py unique.f0.good.mat unique.good.align unique.dbOTU -k 0.0 -p 0.0001 -d 0.1

This should result in the following files (these files are provided in example_data1_res for your reference):

unique.dbOTU.fasta unique.dbOTU.list unique.dbOTU.log unique.dbOTU.mat

Execute the following command in example_data2 to test pre-clustered data option:

python /home/spacocha/lib/dbOTUcaller/dbOTUcaller.py unique.f0.good.mat unique.good.align unique.dbOTU -k 10.0 -p 0.0001 -d 0.1 -s unique.PC.final.list

This should result in the following files (these files are provided in example_data2_res for your reference):

unique.dbOTU.fasta unique.dbOTU.list unique.dbOTU.log unique.dbOTU.mat